Genetic diversity in adzuki bean and its relatives based on SSR markers

doi:10.3724/SP.J.1003.2011.07155

Abstract

Abstract:

In order to enhance the use of genetic resources of Vigna in breeding of adzuki bean, genetic diversity and relationship were analyzed based on 87 SSR markers. The results showed 667 alleles were detected at these SSR loci among 112 accessions of Vigna plants, including 80 cultivated, 22 wild adzuki bean and 10 relative plants. And 75, 71 and 82 were polymorphic in cultivated, wild adzuki bean and relative plants, respectively. Random sampling analysis suggested that the relative plants ranked the first for the average number of alleles per SSR locus, and wild adzuki bean ranked the second, this was agreeable with polymorphism information content (PIC). Cluster analysis divided the cultivated, wild adzuki bean and relative plants into three distinct groups. The genetic background of cultivated adzuki bean basically agreed with their origins. The relative plants could also be divided into three sub-groups, and V. umbellata had different genetic background with other wild species. The present study suggested that wild Vigna had higher genetic variations than cultivated adzuki bean, and could be used to broadening the gene pool of this crop.

Key words: adzuki bean, Vigna, genetic diversity, SSR

Lixia Wang, Xuzhen Cheng, Suhua Wang. Genetic diversity in adzuki bean and its relatives based on SSR markers[J]. Biodiv Sci, 2011, 19(1): 17-23.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2011.07155

https://www.biodiversity-science.net/EN/Y2011/V19/I1/17

Figures/Tables 4

Table 1 Number of accessions detected for different species in Vigna

种属 Species	数量 Amount
Vigna angularis	80
V. angularisvar.nipponensis	22
V. umbellata	3
V. hirtella	1
V. tenuicaulis	1
V. riukivernsis	2
V. minima	1
V. trinervia	1
V. nakashimae	1
合计 Total	112

Fig. 1 Distribution of 667 alleles among cultivated, wild adzuki bean and other relatives. The underlined number is the specific alleles within each population, the bold numbers are the common alleles between populations, and the italic number is the specific alleles among the three populations.

Fig. 2 Dendrogram showing genetic relationship among adzuki bean and its relatives based on coefficient of similarity

Fig. 3 Two-dimentional representaion of genetic relationship among adzuki bean and its relatives

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