Biodiv Sci ›› 2010, Vol. 18 ›› Issue (2): 145-149.DOI: 10.3724/SP.J.1003.2010.150
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Fu Hongtuo1,2,*(), Qiao Hui2, Yao Jianhua2, Gong Yongsheng1, Wu Yan1, Jiang Sufei1, Xiong Yiwei1
Received:
2009-07-07
Accepted:
2010-01-01
Online:
2010-03-20
Published:
2010-03-20
Contact:
Fu Hongtuo
Fu Hongtuo, Qiao Hui, Yao Jianhua, Gong Yongsheng, Wu Yan, Jiang Sufei, Xiong Yiwei. Genetic diversity in five Macrobrachium hainanense populations using SRAP markers[J]. Biodiv Sci, 2010, 18(2): 145-149.
种群代码 Population | 时间 Time | 采样地点 Location |
---|---|---|
瓯江 OJ | 2008.10 | 瓯江双潮段 Shuangchao section, Oujiang River (120.65°E, 28.01°N) |
闽江 MJ | 2008.7 | 闽江福州段 Fuzhou section, Minjiang River (119.30°E, 26.08°N) |
珠江 PR | 2007.4 | 珠江广州段 Guangzhou section, Zhujiang River (113.23°E, 23.16°N) |
昌化江 CH | 2008.9. | 昌化江叉河段 Chahe section, Changhua River (109.00°E, 19.20°N) |
万泉河 WQ | 2008.9. | 万泉河万泉段 Wanquan section, Wan- quan River (110.46°E, 19.25°N) |
Table 1 Samples of Macrobrachium hainanensecollected in this study
种群代码 Population | 时间 Time | 采样地点 Location |
---|---|---|
瓯江 OJ | 2008.10 | 瓯江双潮段 Shuangchao section, Oujiang River (120.65°E, 28.01°N) |
闽江 MJ | 2008.7 | 闽江福州段 Fuzhou section, Minjiang River (119.30°E, 26.08°N) |
珠江 PR | 2007.4 | 珠江广州段 Guangzhou section, Zhujiang River (113.23°E, 23.16°N) |
昌化江 CH | 2008.9. | 昌化江叉河段 Chahe section, Changhua River (109.00°E, 19.20°N) |
万泉河 WQ | 2008.9. | 万泉河万泉段 Wanquan section, Wan- quan River (110.46°E, 19.25°N) |
上游引物 Forward primers | 下游引物 Reverse primers | ||
---|---|---|---|
me1 | 5′-TGAGTCCAAACCGGATA-3′ | em1 | 5′-GACTGCGTACGAATTAAT-3′ |
me2 | 5′-TGAGTCCAAACCGGAGC-3′ | em2 | 5′-GACTGCGTACGAATTTGC-3′ |
me3 | 5′-TGAGTCCAAACCGGAAT-3′ | em3 | 5′-GACTGCGTACGAATTGAC-3′ |
me4 | 5′-TGAGTCCAAACCGGACC-3′ | em4 | 5′-GACTGCGTACGAATTTGA-3′ |
me5 | 5′-TGAGTCCAAACCGGAAG-3′ | em5 | 5′-GACTGCGTACGAATTAAC-3′ |
em6 | 5′-GACTGCGTACGAATTGCA-3′ |
Table 2 SRAP primers used in genetic diversity analysis of Macrobrachium hainanense
上游引物 Forward primers | 下游引物 Reverse primers | ||
---|---|---|---|
me1 | 5′-TGAGTCCAAACCGGATA-3′ | em1 | 5′-GACTGCGTACGAATTAAT-3′ |
me2 | 5′-TGAGTCCAAACCGGAGC-3′ | em2 | 5′-GACTGCGTACGAATTTGC-3′ |
me3 | 5′-TGAGTCCAAACCGGAAT-3′ | em3 | 5′-GACTGCGTACGAATTGAC-3′ |
me4 | 5′-TGAGTCCAAACCGGACC-3′ | em4 | 5′-GACTGCGTACGAATTTGA-3′ |
me5 | 5′-TGAGTCCAAACCGGAAG-3′ | em5 | 5′-GACTGCGTACGAATTAAC-3′ |
em6 | 5′-GACTGCGTACGAATTGCA-3′ |
引物组合Primer combination | 位点总数Total fragments | 引物组合 Primer combination | 位点总数 Total fragments |
---|---|---|---|
me1-em1 | 15 | me3-em6 | 13 |
me1-em3 | 14 | me4-em2 | 13 |
me1-em6 | 24 | me4-em3 | 19 |
me2-em2 | 16 | me4-em6 | 22 |
me2-em3 | 12 | me5-em1 | 19 |
me2-em5 | 14 | me5-em2 | 23 |
me2-em6 | 21 | me5-em4 | 20 |
me3-em2 | 10 |
Table 3 SRAP primer combinations used in genetic variation analysis ofMacrobrachium hainanense
引物组合Primer combination | 位点总数Total fragments | 引物组合 Primer combination | 位点总数 Total fragments |
---|---|---|---|
me1-em1 | 15 | me3-em6 | 13 |
me1-em3 | 14 | me4-em2 | 13 |
me1-em6 | 24 | me4-em3 | 19 |
me2-em2 | 16 | me4-em6 | 22 |
me2-em3 | 12 | me5-em1 | 19 |
me2-em5 | 14 | me5-em2 | 23 |
me2-em6 | 21 | me5-em4 | 20 |
me3-em2 | 10 |
种群 Population | 多态位点数 POL | 多态位点比例 PPB(%) | 遗传杂合度 HE | Shannon信息指数 I |
---|---|---|---|---|
瓯江 OJ | 136 | 53.33% | 0.1839 | 0.2876 |
闽江 MJ | 119 | 46.67% | 0.1892 | 0.2846 |
珠江 PR | 112 | 43.92% | 0.1657 | 0.2543 |
昌化江 CH | 121 | 47.45% | 0.1799 | 0.2746 |
万泉河 WQ | 112 | 43.92% | 0.1763 | 0.2658 |
Table 4 Genetic diversity indices of five geographical populations of Macrobrachium hainanense
种群 Population | 多态位点数 POL | 多态位点比例 PPB(%) | 遗传杂合度 HE | Shannon信息指数 I |
---|---|---|---|---|
瓯江 OJ | 136 | 53.33% | 0.1839 | 0.2876 |
闽江 MJ | 119 | 46.67% | 0.1892 | 0.2846 |
珠江 PR | 112 | 43.92% | 0.1657 | 0.2543 |
昌化江 CH | 121 | 47.45% | 0.1799 | 0.2746 |
万泉河 WQ | 112 | 43.92% | 0.1763 | 0.2658 |
种群名称 Population | 瓯江OJ | 闽江MJ | 珠江PR | 昌化江CH | 万泉河WQ |
---|---|---|---|---|---|
瓯江 OJ | **** | 3.6923 | 5.1366 | 2.7006 | 3.2665 |
闽江 MJ | 0.1193 | **** | 3.9067 | 1.9135 | 3.9127 |
珠江 PR | 0.0887 | 0.1135 | **** | 2.0981 | 3.1088 |
昌化江 CH | 0.1526 | 0.2702 | 0.1924 | **** | 2.0543 |
万泉河 WQ | 0.1327 | 0.1133 | 0.1386 | 0.1958 | **** |
Table 5 Gene flow (Nm, above diagonal) and genetic differentiation (Gst, below diagonal) of five geographical populations of Macrobrachium hainanense
种群名称 Population | 瓯江OJ | 闽江MJ | 珠江PR | 昌化江CH | 万泉河WQ |
---|---|---|---|---|---|
瓯江 OJ | **** | 3.6923 | 5.1366 | 2.7006 | 3.2665 |
闽江 MJ | 0.1193 | **** | 3.9067 | 1.9135 | 3.9127 |
珠江 PR | 0.0887 | 0.1135 | **** | 2.0981 | 3.1088 |
昌化江 CH | 0.1526 | 0.2702 | 0.1924 | **** | 2.0543 |
万泉河 WQ | 0.1327 | 0.1133 | 0.1386 | 0.1958 | **** |
变异来源 Source of variation | 自由度 df | 平方和 Sum of square | 变异组分 Component of variation | 变异贡献率 Contribution rate of variation | P |
---|---|---|---|---|---|
种群间 Among populations | 4 | 257.8857 | 5.5245 | 17.64%** | P<0.001 |
种群内 Within populations | 32 | 774.0000 | 25.8000 | 82.36%** | P<0.001 |
合计 Total | 36 | 1,031.8857 | 31.3245 |
Table 6 AMOVA analysis of genetic variation in five geographical populations of Macrobrachium hainanense based on SRAP markers
变异来源 Source of variation | 自由度 df | 平方和 Sum of square | 变异组分 Component of variation | 变异贡献率 Contribution rate of variation | P |
---|---|---|---|---|---|
种群间 Among populations | 4 | 257.8857 | 5.5245 | 17.64%** | P<0.001 |
种群内 Within populations | 32 | 774.0000 | 25.8000 | 82.36%** | P<0.001 |
合计 Total | 36 | 1,031.8857 | 31.3245 |
种群名称 Populations | 瓯江OJ | 闽江MJ | 珠江PR | 昌化江CH | 万泉河WQ |
---|---|---|---|---|---|
瓯江 OJ | **** | 0.7355 | 0.7528 | 0.6718 | 0.7379 |
闽江 MJ | 0.2645 | **** | 0.7475 | 0.6531 | 0.7406 |
珠江 PR | 0.2472 | 0.2525 | **** | 0.6784 | 0.7446 |
昌化江 CH | 0.3282 | 0.3469 | 0.3216 | **** | 0.6711 |
万泉河 WQ | 0.2621 | 0.2594 | 0.2554 | 0.3289 | **** |
Table 7 Inter-population genetic similarity coefficient (above diagonal) and genetic distance (below diagonal) among five geographical populations of Macrobrachium hainanense
种群名称 Populations | 瓯江OJ | 闽江MJ | 珠江PR | 昌化江CH | 万泉河WQ |
---|---|---|---|---|---|
瓯江 OJ | **** | 0.7355 | 0.7528 | 0.6718 | 0.7379 |
闽江 MJ | 0.2645 | **** | 0.7475 | 0.6531 | 0.7406 |
珠江 PR | 0.2472 | 0.2525 | **** | 0.6784 | 0.7446 |
昌化江 CH | 0.3282 | 0.3469 | 0.3216 | **** | 0.6711 |
万泉河 WQ | 0.2621 | 0.2594 | 0.2554 | 0.3289 | **** |
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