Biodiv Sci ›› 2010, Vol. 18 ›› Issue (2): 145-149.DOI: 10.3724/SP.J.1003.2010.150

Previous Articles     Next Articles

Genetic diversity in five Macrobrachium hainanense populations using SRAP markers

Fu Hongtuo1,2,*(), Qiao Hui2, Yao Jianhua2, Gong Yongsheng1, Wu Yan1, Jiang Sufei1, Xiong Yiwei1   

  1. 1Freshwater Fishery Research Center of Chinese Academy of Fishery Sciences, Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Wuxi, Jiangsu 214081
    2Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu 214081
  • Received:2009-07-07 Accepted:2010-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: Fu Hongtuo

Abstract:

We studied the genetic variation and differentiation of five wild populations of Macrobrachium hainanense using SRAP markers. Samples were collected from Oujiang River (OJ), Minjiang River (MJ), Pearl River (PR), Wanquan River (WQ) and Changhuajiang River (CH). A total of 255 loci were detected, of which 47.05% were polymorphic. Proportion of polymorphic loci of the five populations ranked in the order: PR (43.92%) = WQ (43.92%) < MJ (46.67%) < CH (47.45%) < OJ (53.33%), while heterozygosity ranked as PR (0.1657) < WQ (0. 1763) < CH (0. 179) < OJ (0. 1839) < MJ (0. 1892), with an average of 0.1790. Shannon index ranked as PR (0.2543) < WQ (0.2658) < CH (0.2746) < MJ (0.2846) < OJ (0.2876), with an average of 0.2734. Analysis of molecular variation (AMOVA) revealed that 17.64% of the variance was among populations, which significantly affected the total variance ( P <0.001). Values of Gst, Nm and genetic distance suggested the genetic divergence among populations was relatively high (P < 0.001).

Key words: Macrobrachium hainanense, SRAP, genetic diversity, genetic differentiation, population structure