Genetic diversity in five Macrobrachium hainanense populations using SRAP markers

doi:10.3724/SP.J.1003.2010.150

Abstract

Abstract:

We studied the genetic variation and differentiation of five wild populations of Macrobrachium hainanense using SRAP markers. Samples were collected from Oujiang River (OJ), Minjiang River (MJ), Pearl River (PR), Wanquan River (WQ) and Changhuajiang River (CH). A total of 255 loci were detected, of which 47.05% were polymorphic. Proportion of polymorphic loci of the five populations ranked in the order: PR (43.92%) = WQ (43.92%) < MJ (46.67%) < CH (47.45%) < OJ (53.33%), while heterozygosity ranked as PR (0.1657) < WQ (0. 1763) < CH (0. 179) < OJ (0. 1839) < MJ (0. 1892), with an average of 0.1790. Shannon index ranked as PR (0.2543) < WQ (0.2658) < CH (0.2746) < MJ (0.2846) < OJ (0.2876), with an average of 0.2734. Analysis of molecular variation (AMOVA) revealed that 17.64% of the variance was among populations, which significantly affected the total variance ( P <0.001). Values of G_st, N_m and genetic distance suggested the genetic divergence among populations was relatively high (P < 0.001).

Key words: Macrobrachium hainanense, SRAP, genetic diversity, genetic differentiation, population structure

Hongtuo Fu, Hui Qiao, Jianhua Yao, Yongsheng Gong, Yan Wu, Sufei Jiang, Yiwei Xiong. Genetic diversity in five Macrobrachium hainanense populations using SRAP markers[J]. Biodiv Sci, 2010, 18(2): 145-149.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2010.150

https://www.biodiversity-science.net/EN/Y2010/V18/I2/145

Figures/Tables 8

References 11

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种群代码 Population	时间 Time	采样地点 Location
瓯江 OJ	2008.10	瓯江双潮段 Shuangchao section, Oujiang River (120.65°E, 28.01°N)
闽江 MJ	2008.7	闽江福州段 Fuzhou section, Minjiang River (119.30°E, 26.08°N)
珠江 PR	2007.4	珠江广州段 Guangzhou section, Zhujiang River (113.23°E, 23.16°N)
昌化江 CH	2008.9.	昌化江叉河段 Chahe section, Changhua River (109.00°E, 19.20°N)
万泉河 WQ	2008.9.	万泉河万泉段 Wanquan section, Wan- quan River (110.46°E, 19.25°N)

种群代码 Population	时间 Time	采样地点 Location
瓯江 OJ	2008.10	瓯江双潮段 Shuangchao section, Oujiang River (120.65°E, 28.01°N)
闽江 MJ	2008.7	闽江福州段 Fuzhou section, Minjiang River (119.30°E, 26.08°N)
珠江 PR	2007.4	珠江广州段 Guangzhou section, Zhujiang River (113.23°E, 23.16°N)
昌化江 CH	2008.9.	昌化江叉河段 Chahe section, Changhua River (109.00°E, 19.20°N)
万泉河 WQ	2008.9.	万泉河万泉段 Wanquan section, Wan- quan River (110.46°E, 19.25°N)

上游引物 Forward primers		下游引物 Reverse primers
me1	5′-TGAGTCCAAACCGGATA-3′	em1	5′-GACTGCGTACGAATTAAT-3′
me2	5′-TGAGTCCAAACCGGAGC-3′	em2	5′-GACTGCGTACGAATTTGC-3′
me3	5′-TGAGTCCAAACCGGAAT-3′	em3	5′-GACTGCGTACGAATTGAC-3′
me4	5′-TGAGTCCAAACCGGACC-3′	em4	5′-GACTGCGTACGAATTTGA-3′
me5	5′-TGAGTCCAAACCGGAAG-3′	em5	5′-GACTGCGTACGAATTAAC-3′
		em6	5′-GACTGCGTACGAATTGCA-3′

上游引物 Forward primers		下游引物 Reverse primers
me1	5′-TGAGTCCAAACCGGATA-3′	em1	5′-GACTGCGTACGAATTAAT-3′
me2	5′-TGAGTCCAAACCGGAGC-3′	em2	5′-GACTGCGTACGAATTTGC-3′
me3	5′-TGAGTCCAAACCGGAAT-3′	em3	5′-GACTGCGTACGAATTGAC-3′
me4	5′-TGAGTCCAAACCGGACC-3′	em4	5′-GACTGCGTACGAATTTGA-3′
me5	5′-TGAGTCCAAACCGGAAG-3′	em5	5′-GACTGCGTACGAATTAAC-3′
		em6	5′-GACTGCGTACGAATTGCA-3′

引物组合Primer combination	位点总数Total fragments	引物组合 Primer combination	位点总数 Total fragments
me1-em1	15	me3-em6	13
me1-em3	14	me4-em2	13
me1-em6	24	me4-em3	19
me2-em2	16	me4-em6	22
me2-em3	12	me5-em1	19
me2-em5	14	me5-em2	23
me2-em6	21	me5-em4	20
me3-em2	10