Genetic diversity based on microsatellite markers in five Nile tilapia strains

doi:10.3724/SP.J.1003.2009.08266

Abstract

Abstract:

To provide background on the genetics of cultured Nile tilapia (Oreochromis niloticus) strains and basic information for breeding, we surveyed five Nile tilapia strains (Sudan, Taiwan, GIFT, SupermaleⅠand Supermale Ⅱ) using 19 microsatellite loci to estimate the genetic variation. A total of 113 alleles were detected. Mean expected heterozygosity (H_e) ranged between 0.578 and 0.692, and mean polymorphism information content (PIC) ranged between 0.473 and 0.628, indicating that these five Nile tilapia strains were genetically diverse. Of the five strains, the supermaleⅠhad less genetic variation compared with other strains.

Key words: Oreochromis niloticus, genetic breeding, genetic variation, supermale strains

Hongwei Liang, Zhong Li, Xiangzhong Luo, Changzhong Wang, Guangfu Hu, Guiwei Zou, Yongquan Yang. Genetic diversity based on microsatellite markers in five Nile tilapia strains[J]. Biodiv Sci, 2009, 17(1): 82-87.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2009.08266

https://www.biodiversity-science.net/EN/Y2009/V17/I1/82

Figures/Tables 5

References 12

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品系 Strains	样本量 Sample size	样本来源 Origin
苏丹品系 Sudan (SLF)	48	中国水产科学研究院长江水产研究所 Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences
台湾品系 Taiwan (TLF)	30	厦门鹭业水产有限公司 Xiamen Luye Fisheries Co., Ltd.
超雄品系ⅠSupermale Ⅰ (YYⅠ)	30	厦门鹭业水产有限公司 Xiamen Luye Fisheries Co., Ltd.
超雄品系Ⅱ Supermale Ⅱ (YYⅡ)	30	厦门鹭业水产有限公司 Xiamen Luye Fisheries Co., Ltd.
吉富品系 GIFT	48	上海海洋大学 Shanghai Ocean University

品系 Strains	样本量 Sample size	样本来源 Origin
苏丹品系 Sudan (SLF)	48	中国水产科学研究院长江水产研究所 Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences
台湾品系 Taiwan (TLF)	30	厦门鹭业水产有限公司 Xiamen Luye Fisheries Co., Ltd.
超雄品系ⅠSupermale Ⅰ (YYⅠ)	30	厦门鹭业水产有限公司 Xiamen Luye Fisheries Co., Ltd.
超雄品系Ⅱ Supermale Ⅱ (YYⅡ)	30	厦门鹭业水产有限公司 Xiamen Luye Fisheries Co., Ltd.
吉富品系 GIFT	48	上海海洋大学 Shanghai Ocean University

座位 Locus	引物序列(5'→3′) Primer sequence (5'→3′)	退火温度 Annealing temperature (℃)	片段大小 Allele size (bp)	等位基因数 Number of alleles	登录号 GenBank accession no.
UNH719	F: AAACCATTCATCCTTCACTCG R: GAATGCTTAGTGCCCATCAAT	50	124, 126	8	G63968
UNH735	F: GTGCACACAGCAGAGGCTAA R: CTTTGCTGCTGTCGGAGTTT	60	168, 178, 182	7	G63984
UNH738	F: TGCTGTGCAAATAAAGTGCTG R: TCTTTGCCAGGTTGTCCATT	52	157, 171	11	G63987
UNH773	F: TGGCGGTAAAATGAAAATGG R: CGATCCCAGACTTTGACACA	50	207, 211, 253	6	G64061
UNH817	F: CCTCTTTCTGTGGGTTTCTCC R: CCCAGCCTGCAGTGATACTT	52	99, 119	8	G64035
UNH840	F: TTTCCTGTTCACCCAGTTTT R: GGGCTGAGCAGTCTGGTATT	50	131, 138, 140	3	G68180
UNH841	F: GCGACGCCTACTTCTGAGAG R: TTGTTATTGTCCCTGCTGTCC	58	114, 156	10	G68181
UNH843	F: CGTTCTACTCTGAAGAAAGACATGA R: CCACTCGACGGACGTTTTAG	55	109, 120, 124	6	G68182
UNH844	F: GCCACAATGTCAAGGTTTCA R: GCAGCTGCTCACACACTCTT	58	98, 104, 116	8	G68183
UNH845	F: GCCGACTCCAACTTGCTACT R: ATCCCCTACACGGACAAGTG	55	178, 181	8	G68184
UNH846	F: TGGAGCAGCTTCTTCTACATCA R: CACATGATGGAAGCCGTGTA	55	173, 203	6	G68185
UNH848	F: TCCCCCGTAATAAATTAAACCA R: GCCTGTGAATAACAATGTATTTCCT	55	180, 192, 205	4	G68186
UNH849	F: CCCAGGTGCATATTTCCCTA R: CTTGCTCTGCTTTGCTGAAA	55	155, 191	7	G68187
UNH851	F: TGGCTGTCACATAATCTGGTG R: CCTATCGTCGGTGATTGGTC	55	111, 117, 122	2	G68188
UNH853	F: TAAAGCTCGTCCCCGTAACA R: TGCGTCTCATCACTGTCTGC	55	172, 174, 186	7	G68189
UNH854	F: GGCAAAGAAAGCGATACTGC R: TTCTTAGCTTGTGGTGCACTG	55	225, 229	8	G68190
UNH855	F: ACTCCCGCTGTTGCTGTTAG R: GAGGGGAGCCTACAACGTAA	55	152, 162	3	G68191
UNH856	F: GCAGCCTCACAACATCTCAG R: GATGCATCATCACGGTAACCT	55	189, 197	4	G68192
UNH858	F: TTCAAACAGCTTCACGGTCA R: CTATGCCATGGCTAAAGTCAC	60	257, 280	8	G68194

座位 Locus	引物序列(5'→3′) Primer sequence (5'→3′)	退火温度 Annealing temperature (℃)	片段大小 Allele size (bp)	等位基因数 Number of alleles	登录号 GenBank accession no.
UNH719	F: AAACCATTCATCCTTCACTCG R: GAATGCTTAGTGCCCATCAAT	50	124, 126	8	G63968
UNH735	F: GTGCACACAGCAGAGGCTAA R: CTTTGCTGCTGTCGGAGTTT	60	168, 178, 182	7	G63984
UNH738	F: TGCTGTGCAAATAAAGTGCTG R: TCTTTGCCAGGTTGTCCATT	52	157, 171	11	G63987
UNH773	F: TGGCGGTAAAATGAAAATGG R: CGATCCCAGACTTTGACACA	50	207, 211, 253	6	G64061
UNH817	F: CCTCTTTCTGTGGGTTTCTCC R: CCCAGCCTGCAGTGATACTT	52	99, 119	8	G64035
UNH840	F: TTTCCTGTTCACCCAGTTTT R: GGGCTGAGCAGTCTGGTATT	50	131, 138, 140	3	G68180
UNH841	F: GCGACGCCTACTTCTGAGAG R: TTGTTATTGTCCCTGCTGTCC	58	114, 156	10	G68181
UNH843	F: CGTTCTACTCTGAAGAAAGACATGA R: CCACTCGACGGACGTTTTAG	55	109, 120, 124	6	G68182
UNH844	F: GCCACAATGTCAAGGTTTCA R: GCAGCTGCTCACACACTCTT	58	98, 104, 116	8	G68183
UNH845	F: GCCGACTCCAACTTGCTACT R: ATCCCCTACACGGACAAGTG	55	178, 181	8	G68184
UNH846	F: TGGAGCAGCTTCTTCTACATCA R: CACATGATGGAAGCCGTGTA	55	173, 203	6	G68185
UNH848	F: TCCCCCGTAATAAATTAAACCA R: GCCTGTGAATAACAATGTATTTCCT	55	180, 192, 205	4	G68186
UNH849	F: CCCAGGTGCATATTTCCCTA R: CTTGCTCTGCTTTGCTGAAA	55	155, 191	7	G68187
UNH851	F: TGGCTGTCACATAATCTGGTG R: CCTATCGTCGGTGATTGGTC	55	111, 117, 122	2	G68188
UNH853	F: TAAAGCTCGTCCCCGTAACA R: TGCGTCTCATCACTGTCTGC	55	172, 174, 186	7	G68189
UNH854	F: GGCAAAGAAAGCGATACTGC R: TTCTTAGCTTGTGGTGCACTG	55	225, 229	8	G68190
UNH855	F: ACTCCCGCTGTTGCTGTTAG R: GAGGGGAGCCTACAACGTAA	55	152, 162	3	G68191
UNH856	F: GCAGCCTCACAACATCTCAG R: GATGCATCATCACGGTAACCT	55	189, 197	4	G68192
UNH858	F: TTCAAACAGCTTCACGGTCA R: CTATGCCATGGCTAAAGTCAC	60	257, 280	8	G68194

	吉富品系 GIFT	苏丹品系 Sudan	台湾品系 Taiwan	超雄品系ⅠSupermale Ⅰ	超雄品系Ⅱ Supermale Ⅱ
吉富品系 GIFT	***	0.8336	0.7645	0.7747	0.7429
苏丹品系 Sudan	0.1820	***	0.7565	0.7577	0.7487
台湾品系 Taiwan	0.2685	0.2790	***	0.7829	0.8355
超雄品系Ⅰ Supermale Ⅰ	0.2553	0.2775	0.2448	***	0.8974
超雄品系Ⅱ Supermale Ⅱ	0.2972	0.2895	0.1797	0.1082	***