Biodiv Sci ›› 2008, Vol. 16 ›› Issue (5): 503-508.  DOI: 10.3724/SP.J.1003.2008.07320

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Genetic diversity of the Xuefeng black bone chicken based on microsatellite markers

Wei Lin1, Liu Shenggui2, Shi Xianwei3,*()   

  1. 1 College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128
    2 The Department of Biological Engineering in Huaihua College, Huaihua 418008
    3 College of Animal Science and Technology, Yunnan Agriculture University; The Center for Agricultural Biodiversity and Biotechnology Research and Training of Yunnan Province, Kunming 650201
  • Received:2007-10-31 Accepted:2008-04-01 Online:2008-09-20 Published:2008-09-20
  • Contact: Shi Xianwei


We studied polymorphisms of 23 microsatellite loci from 23 chromosomes in an indigenous breed of Xuefeng black bone chicken from Hunan in order to elucidate its genetic diversity and structure and to propose reasonable measures for its conservation and utilization. Seventy-nine alleles were identified in 50 individuals and the mean number of alleles was 3.435 per locus. Average heterozygosity and polymorphic information content of 23 microsatellite loci were 0.6285 and 0.5496, respectively. These results indicate that this breed of Xuefeng black bone chicken has a high level of genetic diversity and may provide a scientific basis for future conservation and utilization of the Xuefeng black bone chicken.

Key words: Xuefeng black bone chicken, microsatellite markers, polymorphism information content, heterozygosity