PCR-RFLP analysis of large subunit rDNA of symbiotic dinoflagellates in scleractinian corals from Luhuitou fringing reef of Sanya, Hainan

doi:10.3724/SP.J.1003.2008.08074

Abstract

Abstract:

The taxonomy of symbiotic dinoflagellates in scleractinian corals is important in understanding how coral reefs will respond to global climate change. We investigated the composition of the dinoflagellate genus Symbiodinium in 22 species of scleractinian corals from 14 genera and eight families in Luhuitou fringing reef of Sanya, using polymerase chain reaction (PCR) of the large subunit rDNA and restriction fragment length polymorphism (RFLP). The results showed that the dinoflagellate genus Symbiodinium clade C was dominant in the study area. Pocillopora damicornis and Favia favusharbor Symbiodinium Clade D, while Galaxea fascicularis and Acropora horrida can harbor more than one type of symbiont (Symbiodinium Clade C and D). Polymorphic symbiosis suggested that the flexibility of the symbiosis between corals and Symbiodinium. The large subunit rDNA polymorphisms of symbiotic dinoflagellates was lower. Further study is needed to investigate zooxanthellae diversity using other molecular markers.

Key words: South China Sea, scleractinian corals, symbiotic dinoflagellates, DNA polymorphisms

Zhijun Dong, Hui Huang, Liangmin Huang, Yuanchao Li, Xiubao Li. PCR-RFLP analysis of large subunit rDNA of symbiotic dinoflagellates in scleractinian corals from Luhuitou fringing reef of Sanya, Hainan[J]. Biodiv Sci, 2008, 16(5): 498-502.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2008.08074

https://www.biodiversity-science.net/EN/Y2008/V16/I5/498

Figures/Tables 2

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珊瑚种类 Coral species	采样数量 Sample size	共生藻系群 Symbiodinium clade
杯形珊瑚科 Pocilloporidae
杯形珊瑚属 Pocillopora
鹿角杯形珊瑚 P. damicornis	1	D
鹿角珊瑚科 Acroporidae
鹿角珊瑚属 Acropora
丑鹿角珊瑚 A. horrida	1	C & D
浪花鹿角珊瑚 A. cytherea	1	C
多孔鹿角珊瑚 A. millepora	9	C
A. cerealis*	1	C
A. sarmentosa*	1	C
菌珊瑚科 Agariciidae
牡丹珊瑚属 Pavona
十字牡丹珊瑚 P. decussata	2	C
厚丝珊瑚属 Pachyseris
皱纹厚丝珊瑚 P. rugosa	1	C
标准厚丝珊瑚 P. speciosa	1	C
滨珊瑚科 Poritidae
滨珊瑚属 Porites
澄黄滨珊瑚 P. lutea	6	C
扁缩滨珊瑚 P. compressa	1	C
P. murrayensis*	1	C
角孔珊瑚属 Goniopora
细角孔珊瑚 G. gracilis	1	C
二异角孔珊瑚 G. duofasciata	1	C
枇杷珊瑚科 Oculinidae
盔形珊瑚属 Galaxea
丛生盔形珊瑚 G. fascicularis	3	C & D
裸肋珊瑚科 Merulinidae
刺柄珊瑚属 Hydnophora
腐蚀刺柄珊瑚 H. exesa	1	C
蜂巢珊瑚科 Faviidae
蜂巢珊瑚属 Favia
黄癣蜂巢珊瑚 F. favus	1	D
菊花珊瑚属 Goniastrea
梳状菊花珊瑚 G. pectinata	1	C
刺星珊瑚属 Cyphastrea
锯齿刺星珊瑚 C. serailia	1	C
双星珊瑚属 Diploastrea
同双星珊瑚 D. heliopora	1	C
扁脑珊瑚属 Platygyra
中华扁脑珊瑚 P. sinensis	1	C
丁香珊瑚科 Caryophylliidae
泡囊珊瑚属 Plerogyra
泡囊珊瑚 P. sinuosa	1	C

珊瑚种类 Coral species	采样数量 Sample size	共生藻系群 Symbiodinium clade
杯形珊瑚科 Pocilloporidae
杯形珊瑚属 Pocillopora
鹿角杯形珊瑚 P. damicornis	1	D
鹿角珊瑚科 Acroporidae
鹿角珊瑚属 Acropora
丑鹿角珊瑚 A. horrida	1	C & D
浪花鹿角珊瑚 A. cytherea	1	C
多孔鹿角珊瑚 A. millepora	9	C
A. cerealis*	1	C
A. sarmentosa*	1	C
菌珊瑚科 Agariciidae
牡丹珊瑚属 Pavona
十字牡丹珊瑚 P. decussata	2	C
厚丝珊瑚属 Pachyseris
皱纹厚丝珊瑚 P. rugosa	1	C
标准厚丝珊瑚 P. speciosa	1	C
滨珊瑚科 Poritidae
滨珊瑚属 Porites
澄黄滨珊瑚 P. lutea	6	C
扁缩滨珊瑚 P. compressa	1	C
P. murrayensis*	1	C
角孔珊瑚属 Goniopora
细角孔珊瑚 G. gracilis	1	C
二异角孔珊瑚 G. duofasciata	1	C
枇杷珊瑚科 Oculinidae
盔形珊瑚属 Galaxea
丛生盔形珊瑚 G. fascicularis	3	C & D
裸肋珊瑚科 Merulinidae
刺柄珊瑚属 Hydnophora
腐蚀刺柄珊瑚 H. exesa	1	C
蜂巢珊瑚科 Faviidae
蜂巢珊瑚属 Favia
黄癣蜂巢珊瑚 F. favus	1	D
菊花珊瑚属 Goniastrea
梳状菊花珊瑚 G. pectinata	1	C
刺星珊瑚属 Cyphastrea
锯齿刺星珊瑚 C. serailia	1	C
双星珊瑚属 Diploastrea
同双星珊瑚 D. heliopora	1	C
扁脑珊瑚属 Platygyra
中华扁脑珊瑚 P. sinensis	1	C
丁香珊瑚科 Caryophylliidae
泡囊珊瑚属 Plerogyra
泡囊珊瑚 P. sinuosa	1	C