DNA barcode reference dataset for flower-visiting insects in Daiyun Mountain National Nature Reserve

doi:10.17520/biods.2023236

Abstract

Abstract:

Flower-visiting and pollinating insects play vital roles in maintaining ecosystem functions, but the biodiversity data of these insect groups has been grossly inadequate in China. As a species identification method based on specific gene sequences, DNA barcoding has important application value in specimen identification, discovery of new species, biodiversity conservation, population genetics and evolutionary research. In this study, a total of 815 mitochondrial COI barcodes of flower-visiting insects from Diptera, Lepidoptera and Coleoptera in Daiyun Mountain National Nature Reserve were sequenced and analyzed. The distributions of the insect samples at different elevation were also provided in detail. This dataset can help in constructing regional insect DNA barcode databases, discovering cryptic species, studying altitudinal genetic diversity and biodiversity conservation.

Database/Dataset Profile

Title	DNA barcode reference dataset for flower-visiting insects in Daiyun Mountain National Nature Reserve
Author(s)	Xiaoyan Luo, Qiang Li, Xiaolei Huang
Corresponding author	Xiaolei Huang (huangxl@fafu.edu.cn)
Time range	2021
Geographical scope	Dehua County (Fujian), 25°38'-25°43' N, 118°05′-118°20′E
File size	107.63 KB
Data format	.fasta, .xlsx
Data link	http://www.dataopen.info/article/298 http://doi.org/10.24899/do.202307003 https://www.biodiversity-science.net/fileup/1005-0094/DATA/2023236.zip
Database/Dataset composition	The dataset includes a total of 1 sequence data file and 1 sample information file.

Key words: Daiyun Mountain, elevational gradient, Malaise trap, DNA barcode

Xiaoyan Luo, Qiang Li, Xiaolei Huang. DNA barcode reference dataset for flower-visiting insects in Daiyun Mountain National Nature Reserve[J]. Biodiv Sci, 2023, 31(8): 23236.

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URL: https://www.biodiversity-science.net/EN/10.17520/biods.2023236

https://www.biodiversity-science.net/EN/Y2023/V31/I8/23236

Figures/Tables 4

Fig. 1 The location of eight insect monitoring plots in Daiyun Mountain of Fujian

Fig. 2 Proportions of numbers of molecular operational taxonomic units (a) and barcode sequences (b) across different elevational bands

Fig. 3 Species distribution at different elevational bands. The UpSet plot can be divided into the upper (histogram) and the bottom (the bar chart on the left, the elevation in the middle, and the dot plot on the right) parts. In the dot plot, a single point represents the endemic species of a particular group, while connected points represent shared species with other groups. The upper bar chart is used to show the number of shared or unique species among different groups. The bottom left bar chat shows the number of species in different elevations.

Fig. 4 The statistics of BLAST alignment results for DNA barcodes obtained in this study

References 20

[1]	Basset Y, Cizek L, Cuénoud P, Didham RK, Guilhaumon F, Missa O, Novotny V, Ødegaard F, Roslin T, Schmidl J, Tishechkin AK, Winchester NN, Roubik DW, Aberlenc HP, Bail J, Barrios H, Bridle JR, Castaño-Meneses G, Corbara B, Curletti G, DaRocha W, De Bakker D, Delabie JHC, Dejean A, Fagan LL, Floren A, Kitching RL, Medianero E, Miller SE, Oliveira E, Orivel J, Pollet M, Rapp M, Ribeiro SP, Roisin Y, Schmidt JB, Sørensen L, Leponce M (2012) Arthropod diversity in a tropical forest. Science, 338, 1481-1484. DOI PMID
[2]	Bernhardt P (2000) Convergent evolution and adaptive radiation of beetle-pollinated angiosperms. Plant Systematics and Evolution, 222, 293-320. DOI URL
[3]	Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PDN (2006) DNA barcodes distinguish species of tropical Lepidoptera. Proceedings of the National Academy of Sciences, USA, 103, 968-971.
[4]	Hall DM, Martins DJ (2020) Human dimensions of insect pollinator conservation. Current Opinion in Insect Science, 38, 107-114. DOI PMID
[5]	Hall TA (1999) BioEdit:A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
[6]	Hebert PDN, Cywinska A, Ball SL, de Waard JR, (2003a) Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270, 313-321.
[7]	Hebert PDN, Penton EH, Burns JM, Janzen DH, Hallwachs W (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterflyAstraptes fulgerator. Proceedings of the National Academy of Sciences, USA, 101, 14812-14817.
[8]	Hebert PDN, Ratnasingham S, de Waard JR, (2003b) Barcoding animal life: Cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B: Biological Sciences, 270, S96-S99.
[9]	Li Q, Deng J, Chen C, Zeng LD, Lin XL, Cheng ZT, Qiao GX, Huang XL (2019) DNA barcoding subtropical aphids and implications for population differentiation. Insects, 11, 11. DOI URL
[10]	Lin P (2003) Comprehensive Scientific Investigation Report on Daiyun Mountain Nature Reserve in Fujian. Xiamen University Press, Xiamen. (in Chinese)
	[ 林鹏 (2003) 福建戴云山自然保护区综合科学考察报告. 厦门大学出版社, 厦门.]
[11]	Liu YM, Lu XL, Ding SY, Zhou LY, Zhang CC (2020) Distribution patterns of pollination insect community under different agricultural landscape context. Acta Ecologica Sinica, 40, 2376-2385. (in Chinese with English abstract)
	[ 刘娅萌, 卢训令, 丁圣彦, 周立垚, 张晨晨 (2020) 不同农业景观背景下传粉昆虫群落的分布差异. 生态学报, 40, 2376-2385.]
[12]	Lopez-Vaamonde C, Kirichenko N, Cama A, Doorenweerd C, Godfray HCJ, Guiguet A, Gomboc S, Huemer P, Landry JF, Laštůvka A, Laštůvka Z, Lee KM, Lees DC, Mutanen M, van Nieukerken EJ, Segerer AH, Triberti P, Wieser C, Rougerie R (2021) Evaluating DNA barcoding for species identification and discovery in European gracillariid moths. Frontiers in Ecology and Evolution, 9, 626752. DOI URL
[13]	Ollerton J, Winfree R, Tarrant S (2011) How many flowering plants are pollinated by animals? Oikos, 120, 321-326. DOI URL
[14]	Potts SG, Biesmeijer JC, Kremen C, Neumann P, Schweiger O, Kunin WE (2010) Global pollinator declines: Trends, impacts and drivers. Trends in Ecology & Evolution, 25, 345-353. DOI URL
[15]	Rubinoff D (2006) Utility of mitochondrial DNA barcodes in species conservation. Conservation Biology, 20, 1026-1033. PMID
[16]	Saunders ME, Kendall LK, Lanuza JB, Hall MA, Rader R, Stavert JR (2023) Climate mediates roles of pollinator species in plant-pollinator networks. Global Ecology and Biogeography, 32, 511-518. DOI URL
[17]	Ssymank A, Kearns CA, Pape T, Thompson FC (2008) Pollinating flies (Diptera): A major contribution to plant diversity and agricultural production. Biodiversity, 9, 86-89. DOI URL
[18]	Thien LB (1980) Patterns of pollination in the primitive angiosperms. Biotropica, 12, 1-13. DOI URL
[19]	Valentini A, Pompanon F, Taberlet P (2009) DNA barcoding for ecologists. Trends in Ecology & Evolution, 24, 110-117. DOI URL
[20]	Xu SZ, Li ZY, Jin XH (2018) DNA barcoding of invasive plants in China: A resource for identifying invasive plants. Molecular Ecology Resources, 18, 128-136. DOI URL