Biodiv Sci ›› 2020, Vol. 28 ›› Issue (3): 385-400.  DOI: 10.17520/biods.2020045

• Reviews • Previous Articles    

Breeding characteristics and artificial propagation of 14 species of Wild Plant with Extremely Small Populations (WPESP) in China

Sha Deng1,2,Yanni Wu1,2,Kunlin Wu1,Lin Fang1,Lin Li1,Songjun Zeng1,3,*()   

  1. 1 Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650
    2 University of Chinese Academy of Sciences, Beijing 100049
    3 Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650
  • Received:2020-02-17 Accepted:2020-04-27 Online:2020-03-20 Published:2020-04-30
  • Contact: Songjun Zeng


Reproduction is an important part of plant population renewal and maintenance. Threatened species including the Wild Plant with Extremely Small Populations (WPESP), are the result of a combination of internal factors, such as declines in reproductive ability and evolutionary longevity, and external factors, such as artificial over-harvesting and habitat destruction. Efficient artificial reproduction of WPESP can be used for ex situ conservation, reintroduction, and, in some cases, meet the demands of the commodities market, which underscores the need for protection and sustainable use of their germplasm. Seed propagation is the most effective method for maintaining genetic diversity in these speices. However, cutting, grafting and tissue culture are effective supplements for these species that are especially difficult to propagate from seed. Here, we report on the breeding characteristics and propagation methods of 14 typical speices of WPESP selected by our National Key Research Projects of China, and the progress of seedling propagation. A total of 230,000 seedlings from 12 species were produced via sowing, 33,100 seedlings from 5 species via cutting, 2,415 seedlings from 3 species including Manglietiastrum sinicum, Pyrus hopeiensis and Sinojackia huangmeiensis via grafting, 24,850 plantlets from 9 species via tissue culture. The results of this study provide a basis for the protection and use of these 14 species, providing a reference for other WPESP in China.

Key words: Wild Plant with Extremely Small Populations (WPESP), sowing, cutting, grafting, tissue culture