生物多样性 ›› 2002, Vol. 10 ›› Issue (2): 202-207.DOI: 10.17520/biods.2002026

• 论文 • 上一篇    下一篇

石油降解细菌的表型特性和系统发育分析

韩如旸, 闵航, 程志强,柳良好   

  1. 1 浙江大学生命科学学院,杭州 310029
    2 浙江大学生物技术研究所,杭州 310029
    3 杭州娃哈哈集团有限公司,杭州 310020
  • 收稿日期:2001-07-16 修回日期:2001-11-22 出版日期:2002-05-20 发布日期:2002-05-20

Phenotypic characteristics and phylogenetic analysis of crude oil degrading bacteria

HAN Ru-Yang, MIN Hang, CHENG Zhi-Qiang, LIU Liang-Hao   

  1. 1 College of Life Science,Zhejiang University,Hangzhou 310029
    2 Institute of Biotechnology Research,Zhejiang University,Hangzhou 310029
    3 Hangzhou Wahaha Group Ltd.,Hangzhou 310020
  • Received:2001-07-16 Revised:2001-11-22 Online:2002-05-20 Published:2002-05-20

摘要: 从3种不同土壤中分离和纯化得到10个石油降解细菌菌株。分离菌株均为好氧菌、革兰氏阴性菌、不形成芽孢的杆菌。10个菌株均能利用中等链长的烷烃、柴油和原油作为碳源,而不能以单环芳烃和多环芳烃为碳源。根据其生理生化性状和16S rDNA序列分析结果表明,分离菌株EVA5,EVA6,EVA7,EVA8和EVA9为假单胞菌(Pseudomonas spp. ), EVA10、EVA11、EVA12、EVA13和EVA14为不动杆菌 (Acinetobacter spp.), 均属于Proteobacteria 的γ 亚群。

关键词: 入侵种, 入侵生物学, 入侵生态学

Abstract: Ten strains of crude oil degrading bacteria were isolated from three soil samples. The isolates were Gram-negative, aerobic, non-spore-forming rods. All of the ten isolates were able to grow on nalkanes with medium length, diesel fuel and crude oil, but not polycyclic aromatic hydrocarbon. Based on phenotypic characteristics and 16S rDNA sequence analysis, ten isolates were identified as Pseudomona spp. (strain EVA5,EVA6,EVA7,EVA8,EVA9) and Acinetobacter spp. (strain EVA10, EVA11,EVA12,EVA13, EVA14), respectively.

Key words: invasive species, invasive biology, invasive ecology