Biodiversity Science ›› 2010, Vol. 18 ›› Issue (2): 182-187.doi: 10.3724/SP.J.1003.2010.195

• Special Issue • Previous Article     Next Article

Diversity of IAA-producing endophytic bacteria isolated from the roots of Cymbidium goeringii

Lin Liu1; Lei Sun1*; Ruiying Zhang1; Na Yao2; Lubin Li2*   

  1. 1 College of Life Sciences, Hebei University, Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, Hebei 071002

    2 Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091
  • Received:2009-09-15 Online:2010-03-20
  • Lei Sun

Endophytic bacteria can directly stimulate plant growth in several ways, including production of plant hormones such as indole-3-acetic acid (IAA). We investigated the diversity of IAA-producing endophytic bacteria in the roots of Cymbidium goeringii using culture-dependent approaches. A total of 57 IAA-producing strains were obtained from 256 strains isolated from the interior roots of C. goeringii. Based on amplified ribosomal DNA restriction analyses (ARDRA) and 16S rDNA sequences, these 57 strains were divided into 25 groups. The IAA-producing endophytic bacteria were grouped into six phyla, i.e., Alphaproteobacteria (35.1%), Firmicutes (33.3%), Gammaproteobacteria (14.0%), Betaproteobacteria (8.8%), Actinobacteria (7.0%) and Bacteroidetes (1.8%). The dominant groups were Alphaproteobacteria and Firmicutes, and the dominant genus, Paenibacillus, produced relatively high levels of IAA. In addition, four potential novel taxonomic units were found. Our results revealed diverse and abundant communities of IAA-producing endophytic bacteria in the roots of C. goeringii and highlight the microbial resources represented by plant growth-promoting bacteria.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] ZHOU Li-Ying YANG Li-Tao ZHENG Jian-Yu. Recent Progresses in the Studies of Genetic Engineering for Cold Resistance in Plants[J]. Chin Bull Bot, 2001, 18(03): 325 -331 .
[2] HAO Liang-Cheng LIU Chang-Jiang LI Cheng-Sen WANG You-Nian. Advances in the Research on Palaeocarpology[J]. Chin Bull Bot, 2000, 17(专辑): 109 -116 .
[3] LI Guo-Jing ZHOU Xie. Salicylic Acid and Abiotic Stress Resistance in Plants[J]. Chin Bull Bot, 2001, 18(03): 295 -302 .
[4] . [J]. Chin Bull Bot, 1994, 11(专辑): 61 .
[5] Li Shufan. Identification of Drought Resistance in Mung Been at Emergence Stage[J]. Chin Bull Bot, 1986, 4(12): 103 -105 .
[6] Jianting Ding;Jiaheng Shen*;Wei Li;Hong Yang. Cytological Observation of Double Fertilization and Its Duration in Oryza sativa[J]. Chin Bull Bot, 2009, 44(04): 473 -483 .
[7] Yu Tetsun. Book Review: The Bibliography of Chinese Botany. 1857-1981[J]. Chin Bull Bot, 1985, 3(02): 64 .
[8] Ke Shan-qiang;Gui Yao-lin and Guo Zhong-chen. Studies on the Artificial Seeds of Plant[J]. Chin Bull Bot, 1989, 6(04): 205 -210 .
[9] ZHU Xin-Guang and ZHANG Qi-De. Advances in the Research of the Effects of NaCl on Photosynthesis[J]. Chin Bull Bot, 1999, 16(04): 332 -338 .
[10] . [J]. Chin Bull Bot, 2000, 17(04): 384 .