Biodiversity Science ›› 2018, Vol. 26 ›› Issue (12): 1289-1295.doi: 10.17520/biods.2018121

• Original Papers • Previous Article     Next Article

Genetic diversity and population demographic history of Ochetobius elongatus in the middle and lower reaches of the Xijiang River

Jiping Yang1, Ce Li1, 2, Weitao Chen1, Yuefei Li1, Xinhui Li1, *()   

  1. 1 Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380
    2 College of Marine Sciences, Shanghai Ocean University, Shanghai 201306
  • Received:2018-04-16 Accepted:2018-04-16 Online:2019-02-11
  • Li Xinhui E-mail:lxhui01@aliyun.com
  • About author:# 同等贡献作者 Contributed equally to this work

Ochetobius elongatus used to be an economically important fish species in many rivers of China. However, due to environmental destruction and human disturbance the resources of this fish species have reduced rapidly, and its populations are in a critically endangered condition. At present, it is very difficult to obtain specimens of O. elongatus, which limits research on this species. We obtained specimens of O. elongatus via larva and adult fish collection. Two mitochondrial genes and two nuclear genes were used to explore the genetic diversity and population demography of O. elongatus in middle and lower Xijiang River. Results showed that the genetic diversity of O. elongatus was low and is in recession, suggesting that O. elongatus might have undergone a genetic bottleneck event. Additionally, population demographic analyses revealed that O. elongatus population expanded during the Late Pleistocene (0.06 and 0.13 Million years ago), following the glacial retreat of the Middle Pleistocene glaciations (0.78-0.126 Million years ago). The results also revealed that Pleistocene climatic fluctuations have influenced the population demography of O. elongatus. Due to its potential as an important spawning ground for O. elongatus, sections of middle and lower Xijiang River should be considered as candidates for creating nature reserves to conserve and restore its genetic resources.

Key words: Ochetobius elongatus, larva fish collection, mitochondrial and nuclear genes, population expansion, conservation

Table 1

Sample information of the present study"

采样点 Sample site 采样日期 Sampling time 样本量 Sample size 经度 Longitude 纬度 Latitude
广西桂平 Guiping 2017.09 1 110°05°54.78°° E 23°24°08.25°° N
广西梧州 Wuzhou 2017.09 1 111°17°15.32°° E 23°28°07.94°° N
广东肇庆 Zhaoqing 2011.05 22 112°23°40.13°° E 23°05°06.24°° N
广东肇庆 Zhaoqing 2013.05 28 112°23°40.13°° E 23°05°06.24°° N

Table 2

Primer information used in the present study"

引物名 Primer 引物序列 Primer sequence (5′-3′) 退火温度 Annealed temperature (℃) 参考文献 Reference
Cytb L14724 GACTTGAAA AACCACCGTTG 58-64 Xiao et al, 2001
H15915 CTCCGATCTCCGGATTACAAGAC
ND2 AFND2L AAGCTYTYGGGCCCATACC 58 黎瑞宝, 2013
AFND2R TCCYGCTTAGGGCTTTGAAGG
myh6 myh6_F459 CATMTTYTCCATCTCAGATAATGC 53 Chen et al, 2008
myh6_R1325 ATTCTCACCACCATCCAGTTGAA
RAG2 RAG2-f2a AARCGCTCMTGTCCMACTGG 55 Lovejoy & Collette, 2001
RAG2-R6a TGRTCCARGCAGAAGTACTTG

Fig. 1

Median-joining networks of haplotype and allele based on different genes and gene groups. White circles indicate missing haplotype."

Table 3

Genetic diversity indices, neutrality tests and optimal nucleotide substitution model of Ochetobius elongatus"

单倍型数/个体数
Number of haplotype/ sample size
单倍型多态性
Haplotype
diversity
近期核苷酸多态性
Current nucleotide
diversity (θπ, %)
历史核苷酸多态性
Historical nucleotide
diversity (θw, %)
Tajima’s
D 检验
Tajima’s D test
Fu’s Fs检验
Fu’s Fs test
最优模型
Optimal
model
Cytb 13/52 0.784 0.197 0.470 -1.83** -5.43** HKY+I
ND2 17/52 0.869 0.239 0.272 -0.35 -8.16*** F81+I+G
Cytb + ND2 26/52 0.940 0.219 0.366 -1.33 -14.54*** -
myh6 2/52 0.462 0.060 0.029 - - HKY
RAG2 3/52 0.147 0.022 0.077 - - F81
myh6 + RAG2 5/52 0.546 0.040 0.055 - - -

Fig. 2

Mismatch distribution analysis based on different genes and gene groups. Red and purple lines represent observed value and expected values, respectively."

Fig. 3

Extended Bayesian Skyline plots. Black lines represent mean values of effective population size, gray lines represent median values of effective population size, and dashed lines represent 95% confidence interval of effective population size."

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