The impact of anthropogenic noise, artificial light at night and road kills on amphibians

doi:10.17520/biods.2022427

Abstract

Abstract:

Background & Aim: Global biodiversity decline is a major ecological problem around the world today. As an important indicator for measuring the environment, amphibians have received more and more attention from researchers in recent years. In this paper, we focus on amphibians which are the most threatened species of vertebrate to analyze the existing problems and suggest the corresponding solutions.

Method: Firstly, we analyze the effects of three most prominent factors in human activities, i.e., anthropogenic noise, artificial light at night and road kills on amphibians reproduction, population growth rate, physiology, and behavior by retrieving existing literature from 2003 to 2021, and extracting and integrating key words. Secondly, the mitigation measures regarding anthropogenic noise, artificial light at night and road kills are summarized and suggestions for improvement are made.

Review Results: Amphibian calling behavior was altered by anthropogenic noise, showing variations in call rate, dominant frequency, and call duration. It is yet unclear how different amphibians calling respond to anthropogenic noise differently and whether call variations are advantageous to the amphibians’ long-term growth. By obscuring the perception of male acoustic signals by females and impacting sperm count and sperm viability in males, anthropogenic noise can also affect the reproductive behavior of amphibians. Amphibian growth rates and behavioral activity time were slowed down by artificial light at night. In addition, artificial light at night can change corticosterone levels in amphibians and hence have an impact on their physiology. Amphibian population size was directly impacted by road kills. This study makes recommendations for improvement in light of the aforementioned detrimental effects, including bolstering the road infrastructure, constructing noise barriers to muffle noise, reducing light intensity in dense amphibian areas, building amphibian corridors, etc.

Perspective: Specific conservation strategies that seek to improve protection measures, planning management and monitoring of amphibian population dynamics should be implemented in order to reduce the impact of human activities on this group of vertebrates.

Key words: amphibians, human activities, anthropogenic noise, artificial light at night, road kills

Yixin Jiang, Yingying Shi, Shuo Gao, Supen Wang. The impact of anthropogenic noise, artificial light at night and road kills on amphibians[J]. Biodiv Sci, 2023, 31(3): 22427.

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URL: https://www.biodiversity-science.net/EN/10.17520/biods.2022427

https://www.biodiversity-science.net/EN/Y2023/V31/I3/22427

Figures/Tables 2

References 92

[1]	Arévalo JE, Honda W, Arce-Arias A, Häger A (2017) Spatiotemporal variation of roadkills show mass mortality events for amphibians in a highly trafficked road adjacent to a national park, Costa Rica. Revista de Biología Tropical, 65, 1261-1276. DOI URL
[2]	Baker BJ, Richardson JML (2006) The effect of artificial light on male breeding-season behaviour in green frogs, Rana clamitans melanota. Canadian Journal of Zoology, 84, 1528-1532. DOI URL
[3]	Bee MA, Swanson EM (2007) Auditory masking of anuran advertisement calls by road traffic noise. Animal Behaviour, 74, 1765-1776. DOI URL
[4]	Beebee TJC (2013) Effects of road mortality and mitigation measures on amphibian populations. Conservation Biology, 27, 657-668. DOI PMID
[5]	Berger L, Speare R, Daszak P, Green DE, Cunningham AA, Goggin CL, Slocombe R, Ragan MA, Hyatt AD, McDonald KR, Hines HB, Lips KR, Marantelli G, Parkes H (1998) Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America. Proceedings of the National Academy of Sciences, USA, 95, 9031-9036.
[6]	Blaustein AR, Belden LK, Hatch AC, Kats LB, Hoffman PD, Hays JB, Marco A, Chivers DP, Kiesecker JM (2001a) Ultraviolet radiation and amphibians. In: Ecosystems, Evolution and Ultraviolet Radiation (eds Cockell CS, Blaustein AR), pp. 63-79. Springer, New York
[7]	Blaustein AR, Belden LK, Olson DH, Green DM, Root TL, Kiesecker JM (2001b) Amphibian breeding and climate change. Conservation Biology, 15, 1804-1809. DOI URL
[8]	Blaustein AR, Urbina J, Snyder PW, Reynolds E, Dang T, Hoverman JT, Han B, Olson DH, Searle C, Hambalek NM (2018) Effects of emerging infectious diseases on amphibians: A review of experimental studies. Diversity, 10, 81. DOI URL
[9]	Buchanan BW (2006) Observed and potential effects of artificial night lighting on anuran amphibians. In: Ecological Consequences of Artificial Night Lighting (eds Rich C, Longcore T), pp. 192-220. Island Press, Washington, DC.
[10]	Casey RE, Shaw AN, Massal LR, Snodgrass JW (2005) Multimedia evaluation of trace metal distribution within stormwater retention ponds in suburban Maryland, USA. Bulletin of Environmental Contamination and Toxicology, 74, 273-280. PMID
[11]	Chinchar VG (2002) Ranaviruses (family Iridoviridae): Emerg- ing cold-blooded killers. Archives of Virology, 147, 447-470. PMID
[12]	Coelho IP, Teixeira FZ, Colombo P, Coelho AVP, Kindel A (2012) Anuran road-kills neighboring a peri-urban reserve in the Atlantic Forest, Brazil. Journal of Environmental Management, 112, 17-26. DOI PMID
[13]	Coffin AW (2007) From roadkill to road ecology: A review of the ecological effects of roads. Journal of Transport Geography, 15, 396-406. DOI URL
[14]	Cosentino BJ, Marsh DM, Jones KS, Apodaca JJ, Bates C, Beach J, Beard KH, Becklin K, Bell JM, Crockett C, Fawson G, Fjelsted J, Forys EA, Genet KS, Grover M, Holmes J, Indeck K, Karraker NE, Kilpatrick ES, Langen TA, Mugel SG, Molina A, Vonesh JR, Weaver RJ, Willey A (2014) Citizen science reveals widespread negative effects of roads on amphibian distributions. Biological Conservation, 180, 31-38. DOI URL
[15]	Costa AS, Ascensão F, Bager A (2015) Mixed sampling protocols improve the cost-effectiveness of roadkill surveys. Biodiversity and Conservation, 24, 2953-2965. DOI URL
[16]	Cunnington GM, Fahrig L (2010) Plasticity in the vocalizations of anurans in response to traffic noise. Acta Oecologica, 36, 463-470. DOI URL
[17]	Cushman SA (2006) Effects of habitat loss and fragmentation on amphibians: A review and prospectus. Biological Conservation, 128, 231-240. DOI URL
[18]	Cyr NE, Romero LM (2009) Identifying hormonal habituation in field studies of stress. General and Comparative Endocrinology, 161, 295-303. DOI PMID
[19]	Dananay KL, Benard MF (2018) Artificial light at night decreases metamorphic duration and juvenile growth in a widespread amphibian. Proceedings of the Royal Society B: Biological Sciences, 285, 20180367.
[20]	Daversa DR, Muths E, Bosch J (2012) Terrestrial movement patterns of the common toad (Bufo bufo) in central Spain reveal habitat of conservation importance. Journal of Herpetology, 46, 658-664. DOI URL
[21]	Deng K, Zhu BC, Zhou Y, Chen QH, Wang TL, Wang JC, Cui JG (2019) Mate choice decisions of female serrate-legged small treefrogs are affected by ambient light under natural, but not enhanced artificial nocturnal light conditions. Behavioural Processes, 169, 103997. DOI URL
[22]	Dutta H (2018) Insights into the impacts of three current environmental problems on amphibians. European Journal of Ecology, 4, 15-27. DOI URL
[23]	Falchi F, Cinzano P, Duriscoe D, Kyba CCM, Elvidge CD, Baugh K, Portnov BA, Rybnikova NA, Furgoni R (2016) The new world atlas of artificial night sky brightness. Science Advances, 2, e1600377.
[24]	Fei L, Hu SQ, Ye CY, Huang YZ (2006) Fauna Sinica•Amphibian (Vol. 1). Science Press, Beijing. (in Chinese)
	[费梁, 胡淑琴, 叶昌媛, 黄永昭 (2006) 中国动物志•两栖纲(上卷). 科学出版社, 北京.]
[25]	Fellers GM, Wang YZ, Liu SY (2003) Status of amphibians at the Zoige wetlands, Sichuan Province, China. Froglog, 58, 1.
[26]	Forsburg ZR, Guzman A, Gabor CR (2021) Artificial light at night (ALAN) affects the stress physiology but not the behavior or growth of Rana berlandieri and Bufo valliceps. Environmental Pollution, 277, 116775. DOI URL
[27]	Gaston KJ, Bennie J, Davies TW, Hopkins J (2013) The ecological impacts of nighttime light pollution: A mechanistic appraisal. Biological Reviews, 88, 912-927. DOI URL
[28]	Gerhardt HC, Huber F (2003) Acoustic communication in insects and anurans: Common problems and diverse solutions. The Journal of the Acoustical Society of America, 114, 559. DOI URL
[29]	Grant EHC, Muths E, Schmidt BR, Petrovan SO (2019) Amphibian conservation in the Anthropocene. Biological Conservation, 236, 543-547. DOI
[30]	Hamer AJ, McDonnell MJ (2008) Amphibian ecology and conservation in the urbanising world: A review. Biological Conservation, 141, 2432-2449. DOI URL
[31]	Hazell D, Cunnningham R, Lindenmayer D, Mackey B, Osborne W (2001) Use of farm dams as frog habitat in an Australian agricultural landscape: Factors affecting species richness and distribution. Biological Conservation, 102, 155-169. DOI URL
[32]	Heigl F, Horvath K, Laaha G, Zaller JG (2017) Amphibian and reptile road-kills on tertiary roads in relation to landscape structure: Using a citizen science approach with open-access land cover data. BMC Ecology, 17, 1-11. DOI URL
[33]	Helldin JO, Petrovan SO (2019) Effectiveness of small road tunnels and fences in reducing amphibian roadkill and barrier effects at retrofitted roads in Sweden. PeerJ, 7, e7518.
[34]	Hölker F, Moss T, Griefahn B, Kloas W, Voigt CC, Henckel D, Hänel A, Kappeler PM, Völker S, Schwope A, Franke S, Uhrlandt D, Fischer J, Klenke R, Wolter C, Tockner K (2010) The dark side of light: A transdisciplinary research agenda for light pollution policy. Ecology and Society, 15, 13.
[35]	Huijser MP, McGowan P, Hardy A, Kociolek AL, Clevenger AP, Smith D, Ament R (2007) Wildlife-vehicle Collision Reduction Study: Report to Congress U.S. Department of Transportation, Federal Highway Administration, Washington, DC.
[36]	Jägerbrand AK, Bouroussis CA (2021) Ecological impact of artificial light at night: Effective strategies and measures to deal with protected species and habitats. Sustainability, 13, 5991. DOI URL
[37]	Jarvis LE, Hartup M, Petrovan SO (2019) Road mitigation using tunnels and fences promotes site connectivity and population expansion for a protected amphibian. European Journal of Wildlife Research, 65, 27-38. DOI
[38]	Jehle R, Arntzen JW (2000) Post-breeding migrations of newts (Triturus cristatus and T. marmoratus) with contrasting ecological requirements. Journal of Zoology, 251, 297-306. DOI URL
[39]	Jiang JP, Xie F, Zang CX, Cai L, Li C, Wang B, Li JT, Wang J, Hu JH, Wang Y, Liu JY (2016) Assessing the threat status of amphibians in China. Biodiversity Science, 24, 588-597. (in Chinese with English abstract) DOI
	[江建平, 谢锋, 臧春鑫, 蔡蕾, 李成, 王斌, 李家堂, 王杰, 胡军华, 王燕, 刘炯宇 (2016) 中国两栖动物受威胁现状评估. 生物多样性, 24, 588-597.] DOI
[40]	Kaiser K, Devito J, Jones CG, Marentes A, Perez R, Umeh L, Weickum RM, McGovern KE, Wilson EH, Saltzman W (2015) Effects of anthropogenic noise on endocrine and reproductive function in White’s treefrog, Litoria caerulea. Conservation Physiology, 3, cou061.
[41]	Kaiser K, Hammers J (2009) The effect of anthropogenic noise on male advertisement call rate in the neotropical treefrog, Dendropsophus triangulum. Behaviour, 146, 1053-1069. DOI URL
[42]	Kaiser K, Scofield DG, Alloush M, Jones RM, Marczak S, Martineau K, Oliva MA, Narins PM (2011) When sounds collide: The effect of anthropogenic noise on a breeding assemblage of frogs in Belize, Central America. Behaviour, 148, 215-232. DOI URL
[43]	Kazemi VD, Jafari H, Yavari A (2016) Spatio-temporal patterns of wildlife road mortality in Golestan National Park, northeast of Iran. Open Journal of Ecology, 6, 312-324. DOI URL
[44]	Kiesecker JM, Blaustein AR, Miller CL (2001) Potential mechanisms underlying the displacement of native red- legged frogs by introduced bullfrogs. Ecology, 82, 1964-1970. DOI URL
[45]	Lan FY, Ma XJ, Lu JY, Li YG, Chai RS, Li X, Luo YO, Zhang YZ, Ye ZL, Fu CJ, Bao WS, Li L, Xing XY (2021) Effects of urbanization on bird nesting: A review. Biodiversity Science, 29, 1539-1553. (in Chinese with English abstract) DOI
	[蓝方源, 马行健, 逯金瑶, 李雨果, 柴汝松, 李翔, 罗亦欧, 张宇泽, 叶子凌, 付昌健, 暴文爽, 李立, 邢晓莹 (2021) 城市化对鸟类筑巢的影响研究综述. 生物多样性, 29, 1539-1553.] DOI
[46]	Laurance WF, Clements GR, Sloan S, O’Connell CS, Mueller ND, Goosem M, Venter O, Edwards DP, Phalan B, Balmford A, van der Ree R, Arrea IB (2014) A global strategy for road building. Nature, 513, 229-232. DOI
[47]	Lengagne T (2008) Traffic noise affects communication behaviour in a breeding anuran, Hyla arborea. Biological Conservation, 141, 2023-2031. DOI URL
[48]	Li C, Xie F, Che J, Jiang JP (2017) Monitoring and research of amphibians and reptiles diversity in key areas of China. Biodiversity Science, 25, 246-254. (in Chinese with English abstract) DOI
	[李成, 谢锋, 车静, 江建平 (2017) 中国关键地区两栖爬行动物多样性监测与研究. 生物多样性, 25, 246-254.] DOI
[49]	Longcore T, Rich C (2004) Ecological light pollution. Frontiers in Ecology and the Environment, 2, 191-198. DOI URL
[50]	Luo YM, Wang ZC, Wang C, Piao ZJ, Li Z, Huang LY, Zhang R, Sui YC (2015) Survey and conservation of amphibian populations losses in roadside of Changbai Mountain. Journal of Beihua University (Natural Science), 16, 108-112. (in Chinese with English abstract)
	[罗玉梅, 王卓聪, 王超, 朴正吉, 李卓, 黄利亚, 张睿, 睢亚橙 (2015) 长白山路域两栖类动物损失调查及保护. 北华大学学报(自然科学版), 16, 108-112.]
[51]	Maierdiyali A, Wang Y, Tao SC, Kong YP, Wang H, Lü Z (2022) Research status and challenges of road impacts on wildlife in China. Biodiversity Science, 30, 22209. (in Chinese with English abstract) DOI
	[阿卜杜赛麦提·买尔迪亚力, 王云, 陶双成, 孔亚平, 王昊, 吕植 (2022) 我国道路对野生动物影响研究的现状与挑战. 生物多样性, 30, 22209.] DOI
[52]	May D, Shidemantle G, Melnick-Kelley Q, Crane K, Hua J (2019) The effect of intensified illuminance and artificial light at night on fitness and susceptibility to abiotic and biotic stressors. Environmental Pollution, 251, 600-608. DOI PMID
[53]	McKinley DC, Miller-Rushing AJ, Ballard HL, Bonney R, Brown H, Cook-Patton SC, Evans DM, French RA, Parrish JK, Phillips TB, Ryan SF, Shanley LA, Shirk JL, Stepenuck KF, Weltzin JF, Wiggins A, Boyle OD, Briggs RD, Chapin SF III, Hewitt DA, Preuss PW, Soukup MA (2017) Citizen science can improve conservation science, natural resource management, and environmental protection. Biological Conservation, 208, 15-28. DOI URL
[54]	Nelson DV, Klinck H, Carbaugh-Rutland A, Mathis CL, Morzillo AT, Garcia TS (2017) Calling at the highway: The spatiotemporal constraint of road noise on Pacific chorus frog communication. Ecology and Evolution, 7, 429-440. DOI PMID
[55]	O’Donnell RP, Durso AM (2014) Harnessing the power of a global network of citizen herpetologists by improving citizen science databases. Herpetological Review, 45, 151-157.
[56]	Olson DH, Aanensen DM, Ronnenberg KL, Powell CI, Walker SF, Bielby J, Garner TWJ, Weaver G, Fisher MC (2013) Mapping the global emergence of Batrachochytrium dendrobatidis, the amphibian chytrid fungus. PLoS ONE, 8, e56802.
[57]	Parris KM (2002) More bang for your buck: The effect of caller position, habitat and chorus noise on the efficiency of calling in the spring peeper. Ecological Modelling, 156, 213-224. DOI URL
[58]	Perry G, Buchanan B, Fisher R, Salmon M, Wise S (2008) Effects of artigicial night lighting on amphibians and reptiles in urban environments. Herpetological Conservation, 3, 211-228.
[59]	Petrovan SO, Schmidt BR (2016) Volunteer conservation action data reveals large-scale and long-term negative population trends of a widespread amphibian, the common toad (Bufo bufo). PLoS ONE, 11, e0161943.
[60]	Petrovan SO, Schmidt BR (2019) Neglected juveniles; a call for integrating all amphibian life stages in assessments of mitigation success (and how to do it). Biological Conservation, 236, 252-260. DOI
[61]	Petrovan SO, Vale CG, Sillero N (2020) Using citizen science in road surveys for large-scale amphibian monitoring: Are biased data representative for species distribution? Biodiversity and Conservation, 29, 1767-1781.
[62]	Puky M (Irwin CL, 2005) Amphibian road kills: A global perspective. In:Proceedings of the 2005 International Conference on Ecology and Transportation (eds Garrett P, McDermott KP), pp. 325-338. Center for Transportation and the Environment, North Carolina State University, Raleigh.
[63]	Rand AS, Bridarolli ME, Dries L, Ryan MJ (1997) Light levels influence female choice in túngara frogs: Predation risk assessment? Copeia, 2, 447-450.
[64]	Reading CJ (1998) The effect of winter temperatures on the timing of breeding activity in the common toad Bufo bufo. Oecologia, 117, 469-475. DOI PMID
[65]	Romero LM (2004) Physiological stress in ecology: Lessons from biomedical research. Trends in Ecology & Evolution, 19, 249-255. DOI URL
[66]	Rytwinski T, Fahrig L (2015) The impacts of roads and traffic on terrestrial animal populations. In: Handbook of Road Ecology (eds van der Ree R, Smith DJ, Grilo C), pp. 237-246. Springer, New York.
[67]	Rytwinski T, Soanes K, Jaeger JAG, Fahrig L, Findlay CS, Houlahan J, van der Ree R, van der Grift EA (2016) How effective is road mitigation at reducing road-kill? A meta-analysis. PLoS ONE, 11, e0166941.
[68]	Sanchez-Perez JV, Rubio C, Martinez-Sala R, Sanchez-Grandia R, Gomez V (2002) Acoustic barriers based on periodic arrays of scatterers. Applied Physics Letters, 81, 5240-5242. DOI URL
[69]	Santos SM, Carvalho F, Mira A (2011) How long do the dead survive on the road? Carcass persistence probability and implications for road-kill monitoring surveys. PLoS ONE, 6, e25383.
[70]	Sapolsky RM, Romero LM, Munck AU (2000) How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions. Endocrine Reviews, 21, 55-89. DOI PMID
[71]	Shilling F, Perkins SE, Collinson W (2015) Wildlife/roadkill observation and reporting systems. In: Handbook of Road Ecology (eds van der Ree R, Smith DJ, Grilo C), pp. 492-501. John Wiley & Sons, New York.
[72]	Sievers M, Hale R, Parris KM, Melvin SD, Lanctôt CM, Swearer SE (2019a) Contaminant-induced behavioural changes in amphibians: A meta-analysis. Science of the Total Environment, 693, 133570. DOI URL
[73]	Sievers M, Hale R, Swearer SE, Parris KM (2019b) Frog occupancy of polluted wetlands in urban landscapes. Conservation Biology, 33, 389-402. DOI URL
[74]	Simmons AM, Narins PM (2018) Effects of anthropogenic noise on amphibians and reptiles. In: Effects of Anthropogenic Noise on Animals (eds Slabbekoorn H, Dooling R, Popper A, Fay R), pp. 179-208. Springer, New York.
[75]	Skidds DE, Golet FC, Paton PWC, Mitchell JC (2007) Habitat correlates of reproductive effort in wood frogs and spotted salamanders in an urbanizing watershed. Journal of Herpetology, 41, 439-450. DOI URL
[76]	Snodgrass J, Casey R, Massal L (2007) Nitrogen pollution of stormwater ponds: Potential for toxic effects on amphibian embryos and larvae. Applied Herpetology, 4, 19-29. DOI URL
[77]	Sodhi NS, Bickford D, Diesmos AC, Lee TM, Koh LP, Brook BW, Sekercioglu CH, Bradshaw CJA (2008) Measuring the meltdown: Drivers of global amphibian extinction and decline. PLoS ONE, 3, e1636.
[78]	Sterrett SC, Katz RA, Fields WR, Grant EHC (2019) The contribution of road-based citizen science to the conservation of pond-breeding amphibians. Journal of Applied Ecology, 56, 988-995. DOI
[79]	Sun JWC, Narins PM (2005) Anthropogenic sounds differentially affect amphibian call rate. Biological Conservation, 121, 419-427. DOI URL
[80]	Sutherland RW, Dunning PR, Baker WM (2010) Amphibian encounter rates on roads with different amounts of traffic and urbanization. Conservation Biology, 24, 1626-1635. DOI PMID
[81]	Taylor SK, Buergelt CD, Roelke-Parker ME, Homer BL, Rotstein DS (2002) Causes of mortality of free-ranging Florida panthers. Journal of Wildlife Diseases, 38, 107-114. PMID
[82]	Tennessen JB, Parks SE, Langkilde T (2014) Traffic noise causes physiological stress and impairs breeding migration behaviour in frogs. Conservation Physiology, 2, cou032.
[83]	Touzot M, Lengagne T, Secondi J, Desouhant E, Théry M, Dumet A, Duchamp C, Mondy N (2020) Artificial light at night alters the sexual behaviour and fertilisation success of the common toad. Environmental Pollution, 259, 113883. DOI URL
[84]	Touzot M, Teulier L, Lengagne T, Secondi J, Théry M, Libourel PA, Guillard L, Mondy N (2019) Artificial light at night disturbs the activity and energy allocation of the common toad during the breeding period. Conservation Physiology, 7, coz002.
[85]	Underhill VA, Höbel G (2018) Mate choice behavior of female Eastern Gray Treefrogs (Hyla versicolor) is robust to anthropogenic light pollution. Ethology, 124, 537-548. DOI URL
[86]	Creemers R, Joosten K, Donners M, Veenendaal EM (2017) Behaviour of migrating toads under artificial lights differs from other phases of their life cycle. Amphibia-Reptilia, 38, 49-55. DOI URL
[87]	van Renterghem T, Botteldooren D, Verheyen K (2012) Road traffic noise shielding by vegetation belts of limited depth. Journal of Sound and Vibration, 331, 2404-2425. DOI URL
[88]	Vargas-Salinas F, Cunnington GM, Amézquita A, Fahrig L (2014) Does traffic noise alter calling time in frogs and toads? A case study of anurans in Eastern Ontario, Canada. Urban Ecosystems, 17, 945-953. DOI URL
[89]	Wake DB (1991) Declining amphibian populations. Science, 253, 860.
[90]	Yang T, Zhang WY, Dai R, Zheng PY, Shu GC, Zou Q, Li C (2022) Reptile road-kills in Simao District of Pu’er City, Yunnan Province, China. Acta Ecologica Sinica, 42, 2648-2656. (in Chinese with English abstract)
	[杨韬, 章文艳, 戴蓉, 郑普阳, 舒国成, 邹琪, 李成 (2022) 云南省普洱市思茅区路杀爬行动物研究. 生态学报, 42, 2648-2656.]
[91]	Yi YZ, Sheridan JA (2019) Effects of traffic noise on vocalisations of the rhacophorid tree frog Kurixalus chaseni (Anura: Rhacophoridae) in Borneo. Raffles Bulletin of Zoology, 67, 77-82.
[92]	Zhang WY, Shu GC, Li YL, Xiong S, Liang CP, Li C (2018) Daytime driving decreases amphibian roadkill. PeerJ, 6, e5385.

物种 Species	鸣叫频次 Call rate	鸣叫主频 Dominant frequency	鸣叫时长 Call duration	参考文献 Reference
粗皮姬蛙 Microphly butleri	减少 Decrease			Sun & Narins, 2005
黑带蛙 Rana nigrovittata	减少 Decrease			Sun & Narins, 2005
花狭口蛙 Kaloula pulchra	减少 Decrease			Sun & Narins, 2005
台北蛙 Rana taipehensis	增加 Increase			Sun & Narins, 2005
雨蛙 Hyla arborea			减少 Decrease	Lengagne, 2008
蜂黄雨蛙 Dendropsophus triangulum	增加 Increase			Kaiser & Hammers, 2009
豹蛙 Rana pipiens	减少 Decrease	增加 Increase		Cunnington & Fahrig, 2010
变色灰雨蛙 Hyla versicolor	减少 Decrease	不变 No change		Cunnington & Fahrig, 2010
美洲蟾蜍 Bufo americanus	不变 No change	不变 No change		Cunnington & Fahrig, 2010
绿池蛙 Rana clamitans	减少 Decrease	增加 Increase		Cunnington & Fahrig, 2010; Vargas-Salinas et al, 2014
牛蛙 Lithobates catesbeianus	减少 Decrease			Vargas-Salinas et al, 2014
赞氏树蛙 Kurixalus chaseni	不变 No change	不变 No change		Yi & Sheridan, 2019
太平洋岸雨蛙 Pseudacris regilla	不变 No change		不变 No change	Nelson et al, 2017
银瞬红眼蛙 Agalychnis moreletii	不变 No change			Kaiser et al, 2011
斑雨蛙 Tlalocohyla picta	不变 No change			Kaiser et al, 2011
浜岸蟾蜍 Incilius valliceps	不变 No change			Kaiser et al, 2011
丽红眼蛙 Agalychnis callidryas	增加 Increase			Kaiser et al, 2011
红后股雨蛙 Tlalocohyla loquax	增加 Increase			Kaiser et al, 2011
小头雨蛙 Dendropsophus microcephalus	增加 Increase			Kaiser et al, 2011

物种 Species	鸣叫频次 Call rate	鸣叫主频 Dominant frequency	鸣叫时长 Call duration	参考文献 Reference
粗皮姬蛙 Microphly butleri	减少 Decrease			Sun & Narins, 2005
黑带蛙 Rana nigrovittata	减少 Decrease			Sun & Narins, 2005
花狭口蛙 Kaloula pulchra	减少 Decrease			Sun & Narins, 2005
台北蛙 Rana taipehensis	增加 Increase			Sun & Narins, 2005
雨蛙 Hyla arborea			减少 Decrease	Lengagne, 2008
蜂黄雨蛙 Dendropsophus triangulum	增加 Increase			Kaiser & Hammers, 2009
豹蛙 Rana pipiens	减少 Decrease	增加 Increase		Cunnington & Fahrig, 2010
变色灰雨蛙 Hyla versicolor	减少 Decrease	不变 No change		Cunnington & Fahrig, 2010
美洲蟾蜍 Bufo americanus	不变 No change	不变 No change		Cunnington & Fahrig, 2010
绿池蛙 Rana clamitans	减少 Decrease	增加 Increase		Cunnington & Fahrig, 2010; Vargas-Salinas et al, 2014
牛蛙 Lithobates catesbeianus	减少 Decrease			Vargas-Salinas et al, 2014
赞氏树蛙 Kurixalus chaseni	不变 No change	不变 No change		Yi & Sheridan, 2019
太平洋岸雨蛙 Pseudacris regilla	不变 No change		不变 No change	Nelson et al, 2017
银瞬红眼蛙 Agalychnis moreletii	不变 No change			Kaiser et al, 2011
斑雨蛙 Tlalocohyla picta	不变 No change			Kaiser et al, 2011
浜岸蟾蜍 Incilius valliceps	不变 No change			Kaiser et al, 2011
丽红眼蛙 Agalychnis callidryas	增加 Increase			Kaiser et al, 2011
红后股雨蛙 Tlalocohyla loquax	增加 Increase			Kaiser et al, 2011
小头雨蛙 Dendropsophus microcephalus	增加 Increase			Kaiser et al, 2011