Biodiv Sci ›› 2017, Vol. 25 ›› Issue (6): 638-646. DOI: 10.17520/biods.2017060
• Original Papers • Previous Articles Next Articles
Qiujie Zhou1, Yacheng Cai1, Wei Lun Ng1, Wei Wu1, Seping Dai2, Feng Wang3, Renchao Zhou1,*()
Received:
2017-02-27
Accepted:
2017-04-12
Online:
2017-06-20
Published:
2017-07-10
Contact:
Zhou Renchao
Qiujie Zhou, Yacheng Cai, Wei Lun Ng, Wei Wu, Seping Dai, Feng Wang, Renchao Zhou. Molecular evidence for natural hybridization between two Melastoma species endemic to Hainan and their widespread congeners[J]. Biodiv Sci, 2017, 25(6): 638-646.
Fig. 1 Morphological comparison between Melastoma penicillatum, M. candidum and their putative hybrid. (A) M. penicillatum, (B) putative hybrid, (C) M. candidum. Fruits of M. penicillatum (D), putative hybrid (E) and M. candidum (F).
Fig. 2 Morphological comparison between Melastoma dendrisetosum, M. sanguineum and their putative hybrid. Fruits of M. dendrisetosum (A), putative hybrid (B) and M. sanguineum (C). D, Leaves of M. dendrisetosum (left), putative hybrid (middle) and M. sanguineum (right). E, Young twigs of M. dendrisetosum (left), putative hybrid (middle) and M. sanguineum (right).
类群 Taxon | 采样地点 Location | 个体数量 Sample size |
---|---|---|
野牡丹 M. candidum (Cf) | 福建龙海 Longhai, Fujian | 19 |
野牡丹 M. candidum (Ch) | 海南吊罗山 Diaoluoshan, Hainan | 20 |
毛菍 M. sanguineum (S) | 海南吊罗山 Diaoluoshan, Hainan | 20 |
紫毛野牡丹 M. penicillatum (P) | 海南吊罗山 Diaoluoshan, Hainan | 20 |
枝毛野牡丹 M. dendrisetosum (D) | 海南吊罗山 Diaoluoshan, Hainan | 20 |
紫毛野牡丹与野牡丹的嫌疑杂种 Putative hybrid between P and C | 海南吊罗山 Diaoluoshan, Hainan | 1 |
枝毛野牡丹与毛菍的嫌疑杂种 Putative hybrid between D and S | 海南吊罗山 Diaoluoshan, Hainan | 4 |
Table 1 Sampling details of four species of Melastoma and two putative hybrids used in this study
类群 Taxon | 采样地点 Location | 个体数量 Sample size |
---|---|---|
野牡丹 M. candidum (Cf) | 福建龙海 Longhai, Fujian | 19 |
野牡丹 M. candidum (Ch) | 海南吊罗山 Diaoluoshan, Hainan | 20 |
毛菍 M. sanguineum (S) | 海南吊罗山 Diaoluoshan, Hainan | 20 |
紫毛野牡丹 M. penicillatum (P) | 海南吊罗山 Diaoluoshan, Hainan | 20 |
枝毛野牡丹 M. dendrisetosum (D) | 海南吊罗山 Diaoluoshan, Hainan | 20 |
紫毛野牡丹与野牡丹的嫌疑杂种 Putative hybrid between P and C | 海南吊罗山 Diaoluoshan, Hainan | 1 |
枝毛野牡丹与毛菍的嫌疑杂种 Putative hybrid between D and S | 海南吊罗山 Diaoluoshan, Hainan | 4 |
类群 Taxon | cam | tpi | |||||||
---|---|---|---|---|---|---|---|---|---|
292 | 244 | 204 | 225 | 258 | 272 | 315 | 340 | 568 | |
野牡丹 Melastoma candidum (C) | A | G | T | T | T | G | A | T | C |
紫毛野牡丹 M. penicillatum (P) | G | A | A | C | C | A | G | C | T |
紫毛野牡丹和野牡丹的嫌疑杂种 Putative hybrid between P and C | R | R | W | Y | Y | R | R | Y | Y |
Table 2 The base composition of the differentially fixed sites at the cam and tpi genes between Melastoma penicillatum and M. candidum in their putative hybrid (R = A + G; Y = C + T; W = T + A)
类群 Taxon | cam | tpi | |||||||
---|---|---|---|---|---|---|---|---|---|
292 | 244 | 204 | 225 | 258 | 272 | 315 | 340 | 568 | |
野牡丹 Melastoma candidum (C) | A | G | T | T | T | G | A | T | C |
紫毛野牡丹 M. penicillatum (P) | G | A | A | C | C | A | G | C | T |
紫毛野牡丹和野牡丹的嫌疑杂种 Putative hybrid between P and C | R | R | W | Y | Y | R | R | Y | Y |
类群 Taxon | cam | chi | gbss | tpi | ||||||
---|---|---|---|---|---|---|---|---|---|---|
190 | 329 | 661 | 79 | 258 | 315 | 414 | 80 | 240 | 286 | |
毛菍 M. sanguineum (S) | T | C | G | C | A | G | G | G | T | A |
枝毛野牡丹 M. dendrisetosum (D) | C | T | A | T | C | A | C | A | C | T |
枝毛野牡丹和毛菍的嫌疑杂种 Putative hybrid between D and S | Y | Y | R | Y | M | R | S | R | Y | W |
Table 3 The base composition of the differentially fixed sites at the four nuclear genes between Melastoma dendrisetosum and M. sanguineum in their putative hybrid (R = A + G; Y = C + T; W = T + A; M = A + C; S = C + G)
类群 Taxon | cam | chi | gbss | tpi | ||||||
---|---|---|---|---|---|---|---|---|---|---|
190 | 329 | 661 | 79 | 258 | 315 | 414 | 80 | 240 | 286 | |
毛菍 M. sanguineum (S) | T | C | G | C | A | G | G | G | T | A |
枝毛野牡丹 M. dendrisetosum (D) | C | T | A | T | C | A | C | A | C | T |
枝毛野牡丹和毛菍的嫌疑杂种 Putative hybrid between D and S | Y | Y | R | Y | M | R | S | R | Y | W |
Fig. 3 Median-joining networks of tpi (A), gbss (B), chi (C) and cam (D) genes of the four Melastoma species and the two putative hybrids. The numbers around the connecting lines between haplotypes represent the number of mutational steps between them, while those without numbers represent only one mutational step. Small black circles represent hypothetical or unsampled haplotypes.
类群 Taxon | 个体编号 Sample ID | tpi | cam | chi | gbss |
---|---|---|---|---|---|
紫毛野牡丹和野牡丹的嫌疑杂种 Putative hybrid (PC) | PC | PC1PC2 | PC1PC2 | PC1PC2 | PC1 PC2 |
枝毛野牡丹和毛菍的嫌疑杂种 Putative hybrid (DS) | DS_1 | DS1DS2 | DS1DS4 | DS1 DS2 | DS1DS2 |
DS_2 | DS1DS3 | DS2DS4 | DS1DS3 | DS3DS4 | |
DS_3 | DS1DS4 | DS3DS5 | DS3DS4 | DS1DS2 | |
DS_4 | DS1DS5 | DS2DS4 | DS1DS3 | DS1DS5 |
Table 4 Genotypes of two putative hybrids of Melastoma at four nuclear genes (tpi, cam, chi and gbss). Haplotypes with single, double, wavy, or dotted underlines have identical sequences with those of M. candidum, M. penicillatum, M. sanguineum, and M. dendrisetosum, respectively. Haplotypes without underline are unique to the putative hybrids.
类群 Taxon | 个体编号 Sample ID | tpi | cam | chi | gbss |
---|---|---|---|---|---|
紫毛野牡丹和野牡丹的嫌疑杂种 Putative hybrid (PC) | PC | PC1PC2 | PC1PC2 | PC1PC2 | PC1 PC2 |
枝毛野牡丹和毛菍的嫌疑杂种 Putative hybrid (DS) | DS_1 | DS1DS2 | DS1DS4 | DS1 DS2 | DS1DS2 |
DS_2 | DS1DS3 | DS2DS4 | DS1DS3 | DS3DS4 | |
DS_3 | DS1DS4 | DS3DS5 | DS3DS4 | DS1DS2 | |
DS_4 | DS1DS5 | DS2DS4 | DS1DS3 | DS1DS5 |
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