Biodiv Sci ›› 2012, Vol. 20 ›› Issue (4): 451-459. DOI: 10.3724/SP.J.1003.2012.09221
• Original Papers • Previous Articles Next Articles
Rong Zhou1, Jiaqi Li2, You Li3, Naifa Liu2,*(), Fengjie Fang2, Limin Shi2, Ying Wang2
Received:
2011-12-20
Accepted:
2012-04-11
Online:
2012-07-20
Published:
2012-09-12
Contact:
Naifa Liu
Rong Zhou, Jiaqi Li, You Li, Naifa Liu, Fengjie Fang, Limin Shi, Ying Wang. Genetic variation in rusty-necklaced partridge (Alectoris magna) detected by mitochondrial DNA[J]. Biodiv Sci, 2012, 20(4): 451-459.
种群 Populations | 经纬度 Locality | 所属亚种 Subspecies | 样本量 Sample size | ||
---|---|---|---|---|---|
控制区 D-loop | 细胞色素b CYTB | ||||
青海 Qinghai | |||||
德令哈 Delingha (DLH) | 37.22°N, 97.37°E | A. m. magna | 12 | 12 | |
乌兰 Wulan (WL) | 36.93°N, 98.48°E | A. m. magna | 18 | 23 | |
都兰 Dulan (DL) | 36.30°N, 98.10°E | A. m. magna | 25 | 34 | |
共和 Gonghe (GH) | 36.28°N, 100.62°E | A. m. lanzhouensis | 21 | 20 | |
贵德 Guide (GD) | 35.93°N, 101.48°E | A. m. lanzhouensis | 11 | 12 | |
互助 Huzhu (HZ) | 36.85°N, 102.15°E | A. m. lanzhouensis | 16 | 15 | |
民和 Minhe (MH) | 36.33°N, 102.80°E | A. m. lanzhouensis | 12 | 12 | |
甘肃 Gansu 靖远 Jingyuan (JY) | 36.34°N, 104.81°E | A. m. lanzhouensis | 12 | 40 | |
会宁 Huining (HN) | 35.91°N, 104.80°E | A. m. lanzhouensis | 8 | 16 | |
礼县 Lixian (LX) | 34.20°N, 105.13°E | A. m. lanzhouensis | 14 | 18 | |
张家川 Zhangjiachuan (ZJC) | 34.99°N, 106.21°E | A. m. lanzhouensis | 9 | 10 | |
宁夏 Ningxia 海原 Haiyuan (HY) | 36.57°N, 105.64°E | A. m. lanzhouensis | 15 | 18 | |
总计 Total | 173 | 230 |
Table 1 Sampling information for 12 populations of Alectoris magna
种群 Populations | 经纬度 Locality | 所属亚种 Subspecies | 样本量 Sample size | ||
---|---|---|---|---|---|
控制区 D-loop | 细胞色素b CYTB | ||||
青海 Qinghai | |||||
德令哈 Delingha (DLH) | 37.22°N, 97.37°E | A. m. magna | 12 | 12 | |
乌兰 Wulan (WL) | 36.93°N, 98.48°E | A. m. magna | 18 | 23 | |
都兰 Dulan (DL) | 36.30°N, 98.10°E | A. m. magna | 25 | 34 | |
共和 Gonghe (GH) | 36.28°N, 100.62°E | A. m. lanzhouensis | 21 | 20 | |
贵德 Guide (GD) | 35.93°N, 101.48°E | A. m. lanzhouensis | 11 | 12 | |
互助 Huzhu (HZ) | 36.85°N, 102.15°E | A. m. lanzhouensis | 16 | 15 | |
民和 Minhe (MH) | 36.33°N, 102.80°E | A. m. lanzhouensis | 12 | 12 | |
甘肃 Gansu 靖远 Jingyuan (JY) | 36.34°N, 104.81°E | A. m. lanzhouensis | 12 | 40 | |
会宁 Huining (HN) | 35.91°N, 104.80°E | A. m. lanzhouensis | 8 | 16 | |
礼县 Lixian (LX) | 34.20°N, 105.13°E | A. m. lanzhouensis | 14 | 18 | |
张家川 Zhangjiachuan (ZJC) | 34.99°N, 106.21°E | A. m. lanzhouensis | 9 | 10 | |
宁夏 Ningxia 海原 Haiyuan (HY) | 36.57°N, 105.64°E | A. m. lanzhouensis | 15 | 18 | |
总计 Total | 173 | 230 |
种群 Population | 控制区 D-loop | 细胞色素 b CYTB | |||
---|---|---|---|---|---|
h | π | h | π | ||
德令哈 DLH | 0.652 ± 0.133 | 0.00257 ± 0.00046 | 0.000 ± 0.000 | 0.00000 ± 0.00000 | |
乌兰 WL | 0.712 ± 0.074 | 0.00370 ± 0.00040 | 0.553 ± 0.080 | 0.00158 ± 0.00029 | |
都兰 DL | 0.663 ± 0.075 | 0.00386 ± 0.00046 | 0.508 ± 0.055 | 0.00124 ± 0.00014 | |
共和 GH | 0.800 ± 0.062 | 0.00342 ± 0.00035 | 0.763 ± 0.059 | 0.00202 ± 0.00022 | |
贵德 GD | 0.818 ± 0.119 | 0.00329 ± 0.00068 | 0.727 ± 0.113 | 0.00244 ± 0.00037 | |
互助 HZ | 0.700 ± 0.080 | 0.00303 ± 0.00068 | 0.467 ± 0.148 | 0.00090 ± 0.00032 | |
民和 MH | 0.894 ± 0.063 | 0.00330 ± 0.00055 | 0.591 ± 0.108 | 0.00207 ± 0.00037 | |
靖远 JY | 0.894 ± 0.063 | 0.00436 ± 0.00062 | 0.708 ± 0.062 | 0.00193 ± 0.00022 | |
会宁 HN | 0.750 ± 0.139 | 0.00219 ± 0.00105 | 0.508 ± 0.126 | 0.00154 ± 0.00036 | |
礼县 LX | 0.495 ± 0.151 | 0.00156 ± 0.00055 | 0.745 ± 0.079 | 0.00197 ± 0.00041 | |
张家川 ZJC | 0.694 ± 0.147 | 0.00197 ± 0.00064 | 0.644 ± 0.152 | 0.00173 ± 0.00054 | |
海原 HY | 0.476 ± 0.155 | 0.00208 ± 0.00087 | 0.471 ± 0.130 | 0.00116 ± 0.00045 | |
平均 Average | 0.916 ± 0.011 | 0.00449 ± 0.00242 | 0.738 ± 0.024 | 0.00216 ± 0.00009 |
Table 2 Haplotype diversity (h) and nucleotide diversity (π) for 12 populations of Alectoris magna
种群 Population | 控制区 D-loop | 细胞色素 b CYTB | |||
---|---|---|---|---|---|
h | π | h | π | ||
德令哈 DLH | 0.652 ± 0.133 | 0.00257 ± 0.00046 | 0.000 ± 0.000 | 0.00000 ± 0.00000 | |
乌兰 WL | 0.712 ± 0.074 | 0.00370 ± 0.00040 | 0.553 ± 0.080 | 0.00158 ± 0.00029 | |
都兰 DL | 0.663 ± 0.075 | 0.00386 ± 0.00046 | 0.508 ± 0.055 | 0.00124 ± 0.00014 | |
共和 GH | 0.800 ± 0.062 | 0.00342 ± 0.00035 | 0.763 ± 0.059 | 0.00202 ± 0.00022 | |
贵德 GD | 0.818 ± 0.119 | 0.00329 ± 0.00068 | 0.727 ± 0.113 | 0.00244 ± 0.00037 | |
互助 HZ | 0.700 ± 0.080 | 0.00303 ± 0.00068 | 0.467 ± 0.148 | 0.00090 ± 0.00032 | |
民和 MH | 0.894 ± 0.063 | 0.00330 ± 0.00055 | 0.591 ± 0.108 | 0.00207 ± 0.00037 | |
靖远 JY | 0.894 ± 0.063 | 0.00436 ± 0.00062 | 0.708 ± 0.062 | 0.00193 ± 0.00022 | |
会宁 HN | 0.750 ± 0.139 | 0.00219 ± 0.00105 | 0.508 ± 0.126 | 0.00154 ± 0.00036 | |
礼县 LX | 0.495 ± 0.151 | 0.00156 ± 0.00055 | 0.745 ± 0.079 | 0.00197 ± 0.00041 | |
张家川 ZJC | 0.694 ± 0.147 | 0.00197 ± 0.00064 | 0.644 ± 0.152 | 0.00173 ± 0.00054 | |
海原 HY | 0.476 ± 0.155 | 0.00208 ± 0.00087 | 0.471 ± 0.130 | 0.00116 ± 0.00045 | |
平均 Average | 0.916 ± 0.011 | 0.00449 ± 0.00242 | 0.738 ± 0.024 | 0.00216 ± 0.00009 |
变异来源 Source of variations | 变异组成 Variance components | 变异百分比 % of variation | P | 固定指数(99% IC) Fixation indices |
---|---|---|---|---|
D-loop | ||||
地理群组间 Among groups | 0.279 | 10.21 | 0.041 | FCT = 0.102 |
地理种群间 Among populations within groups | 0.534 | 19.54 | 0.000 | FSC = 0.218 |
种群内 Within populations | 1.921 | 70.25 | 0.000 | FST = 0.298 |
CYTB | ||||
地理群组间 Among groups | 0.120 | 12.49 | 0.038 | FCT = 0.125 |
地理种群间 Among populations within groups | 0.205 | 21.33 | 0.000 | FSC = 0.244 |
种群内 Within populations | 0.635 | 66.18 | 0.000 | FST = 0.338 |
Table 3 Analysis of molecular variance (AMOVA) of Alectoris magna based on partial sequences of D-loop and CYTB
变异来源 Source of variations | 变异组成 Variance components | 变异百分比 % of variation | P | 固定指数(99% IC) Fixation indices |
---|---|---|---|---|
D-loop | ||||
地理群组间 Among groups | 0.279 | 10.21 | 0.041 | FCT = 0.102 |
地理种群间 Among populations within groups | 0.534 | 19.54 | 0.000 | FSC = 0.218 |
种群内 Within populations | 1.921 | 70.25 | 0.000 | FST = 0.298 |
CYTB | ||||
地理群组间 Among groups | 0.120 | 12.49 | 0.038 | FCT = 0.125 |
地理种群间 Among populations within groups | 0.205 | 21.33 | 0.000 | FSC = 0.244 |
种群内 Within populations | 0.635 | 66.18 | 0.000 | FST = 0.338 |
Fig. 1 Neighbour-joining tree of Tamura-Nei’s distance between 44 mtDNA D-loop haplotypes. Numbers indicate the percentage con?dence level of each node estimated by 1,000 bootstrap samplings of the data. Population codes see Table 1.
Fig. 2 Neighbour-joining tree of Tamura-Nei’s distance between 14 mtDNA CYTB haplotypes. Numbers indicate the percentage con?dence level of each node estimated by 1,000 bootstrap samplings of the data. Population codes see Table 1.
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