Mitochondrial DNA D-loop sequence analysis and population rejuvena- tion of Hainan gibbons (Nomascus hainanus)

doi:10.3724/SP.J.1003.2010.523

Abstract

Abstract:

The Hainan gibbon (Nomascus hainanus) is one among the most endangered primates globally according to Critically Endangered List of IUCN. However, there has been no report on its genetic information and the difficulties of its population restoration. This study represents the first time survey of genetic diversity using mitochondrial D-loop sequences from faecal sampling of six individuals within one group. Five variable sites were detected in the 202 bp D-loop sequences and 4 haplotypes were defined. The haplotype diversity (h) and nucleotide diversity (π) were 0.6000 and 0.00829, respectively, meaning the genetic diversity was low. Furthermore, this critically endangered species also face other threatening factors, such as small population size, biased sex ratio and low habitat quality.

Key words: Nomascus hainanus, faecal DNA, mitochondrial DNA D-loop, genetic diversity

Zhigang Li, Fuwen Wei, Jiang Zhou. Mitochondrial DNA D-loop sequence analysis and population rejuvena- tion of Hainan gibbons (Nomascus hainanus)[J]. Biodiv Sci, 2010, 18(5): 523-527.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2010.523

https://www.biodiversity-science.net/EN/Y2010/V18/I5/523

Figures/Tables 2

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Indiv- idual codes	变异位点 Variable sites
Indiv- idual codes	1-10	11-20	21-30	31-40	41-50	51-60	61-70	71-80	81-90
1	TAGAACATCC	CCTCCCCATT	TCAACATTCC	AAACCTACCC	AACATGCGTA	TCAACCAACC	AAGATAGTCC	ATCTCGGACA	TGGCACATTA
2	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
3	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - A- - - - - - -
4	- - - G - - - - - -	- - - TT - .- - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
5	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
6	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -

Indiv- idual codes	变异位点 Variable sites
Indiv- idual codes	1-10	11-20	21-30	31-40	41-50	51-60	61-70	71-80	81-90
1	TAGAACATCC	CCTCCCCATT	TCAACATTCC	AAACCTACCC	AACATGCGTA	TCAACCAACC	AAGATAGTCC	ATCTCGGACA	TGGCACATTA
2	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
3	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - A- - - - - - -
4	- - - G - - - - - -	- - - TT - .- - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
5	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
6	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -