Population genetic structure of tapertail anchovy (Coilia mystus) in coastal waters of southeast China based on mtDNA control region sequences

doi:10.3724/SP.J.1003.2009.08286

Abstract

Abstract:

To analyze the genetic diversity and genetic structure of Coilia mystus, 65 individuals were sampled from four localities, including the Minjiang (MJ), Jiulongjiang (JL), Yangtze (CJ) and Qiantang rivers (QT). Mitochondrial DNA variation was analyzed using 561 bp segments of control region, among which 28 haplotypes were detected. The CJ population had the highest nucleotide diversity (0.0080 ± 0.0046) while the MJ population had the lowest (0.0015 ± 0.0013). Overall haplotype and nucleotide diversity were 0.9433 ± 0.0168 and 0.0317 ± 0.0158, respectively. Average pairwise Kimura 2-parameter distances between MJ and JL and between CJ and QT were 0.3% and 0.8%, respectively, while those between MJ and JL as well between CJ and QT were 6%. Phylogenetic trees constructed using maximum likelihood (ML), maximum parsimony (MP) and neighbor-joining (NJ) methods showed similar topology with two well-supported monophyletic groups. The haplotypes from MJ and JL formed a monophyletic group sister to that comprising the haplotypes of CJ and QT. This interrelationship was supported by network analysis with 28 mutation steps between the two monophyletic clades. Analysis of molecular variance (AMOVA) revealed that the variation between the MJ-JL and CJ-QT clades accounted for 90.77% of total variation, suggesting that significant geographical division was present. The low level of gene flow (N_m< 0.03) and high population differentiation values (F_ST > 0.9) of the two clades also showed considerable genetic isolation. Our results suggest that these four C. mystus populations are divided into two geographical units, and that these units might be considered at the subspecies level in terms of genetic data. The molecular clock estimated using BEAST and TRACER softwares indicated that the subdivision of C. mystus occurred in the late Pleistocene (about 0.34-0.46 million years BP). Climatic oscillations and recurrent marine regression during this period may have influenced the geographical isolation and genetic differentiation of C. mystus.

Key words: Coilia mystus, coastal waters of southeast China, mtDNA control region, genetic diversity, monophyletic group

Xuelan Yan, Wenqiao Tang, Jinquan Yang*. Population genetic structure of tapertail anchovy (Coilia mystus) in coastal waters of southeast China based on mtDNA control region sequences[J]. Biodiv Sci, 2009, 17(2): 143-150.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2009.08286

https://www.biodiversity-science.net/EN/Y2009/V17/I2/143

Figures/Tables 6

References 32

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采集地 Location	样本数Sample size	单元型数 Haplotype number	单元型 Haplotype	单元型多样性Haplotype diversity (h)	核苷酸多样性 Nucleotide diversity (π)
闽江 Minjiang River (MJ)	17	6	H1(1)、H2(1)、H4(2)、H5(1)、H7(1)、H8(11)	0.5882 ± 0.1348	0.0015 ± 0.0013
九龙江 Jiulong River (JL)	16	7	H1(1)、H3(1)、H6(1)、H8(10)、H9(1)、H10(1)、H11(1)	0.6250 ± 0.1390	0.0037 ± 0.0024
长江 Yangtze River (CJ)	18	11	H12(4)、H13(1)、H14(1)、H15(1)、H19(3)、H20(1)、H21(1)、H22(1)、H23(3)、H25(1)、 H27(1)	0.9216 ± 0.0417	0.0080 ± 0.0046
钱塘江 Qiantang River (QT)	14	10	H14(1)、H16(1)、H17(1)、H18(1)、H19(3)、H22(2)、H23(2)、H24(1)、H26(1)、H28(1)	0.9451 ± 0.0451	0.0077 ± 0.0046
总计 Total	65	28		0.9433 ± 0.0168	0.0317 ± 0.0158

采集地 Location	样本数Sample size	单元型数 Haplotype number	单元型 Haplotype	单元型多样性Haplotype diversity (h)	核苷酸多样性 Nucleotide diversity (π)
闽江 Minjiang River (MJ)	17	6	H1(1)、H2(1)、H4(2)、H5(1)、H7(1)、H8(11)	0.5882 ± 0.1348	0.0015 ± 0.0013
九龙江 Jiulong River (JL)	16	7	H1(1)、H3(1)、H6(1)、H8(10)、H9(1)、H10(1)、H11(1)	0.6250 ± 0.1390	0.0037 ± 0.0024
长江 Yangtze River (CJ)	18	11	H12(4)、H13(1)、H14(1)、H15(1)、H19(3)、H20(1)、H21(1)、H22(1)、H23(3)、H25(1)、 H27(1)	0.9216 ± 0.0417	0.0080 ± 0.0046
钱塘江 Qiantang River (QT)	14	10	H14(1)、H16(1)、H17(1)、H18(1)、H19(3)、H22(2)、H23(2)、H24(1)、H26(1)、H28(1)	0.9451 ± 0.0451	0.0077 ± 0.0046
总计 Total	65	28		0.9433 ± 0.0168	0.0317 ± 0.0158

种群 Population	闽江 MJ	九龙江 JL	长江 CJ	钱塘江 QT
闽江 MJ	0.002
九龙江 JL	0.003	0.004
长江 CJ	0.060	0.060	0.007
钱塘江 QT	0.060	0.059	0.008	0.008

种群 Population	闽江 MJ	九龙江 JL	长江 CJ	钱塘江 QT
闽江 MJ	0.002
九龙江 JL	0.003	0.004
长江 CJ	0.060	0.060	0.007
钱塘江 QT	0.060	0.059	0.008	0.008

变异来源 Source of variation	自由度 df	变异分量 Variance components	变异百分比 Percentage of variation
组间 Among groups	1	14.59231	90.77
组内种群间 Among populations within group	2	0.03239	0.20
种群内 Within population	61	1.45064	9.02
总计 Total	64	16.07535