转BPH9和Bar基因抗褐飞虱耐除草剂水稻‘H23’对非靶标生物的影响

doi:10.17520/biods.2020295

摘要/Abstract

摘要：

为探明转BPH9和Bar基因抗褐飞虱耐除草剂水稻‘H23’对非靶标生物安全性的影响, 本文以‘H23’的非转基因亲本改良的‘广占63-4S’为对照, 比较二者对二化螟(Chilo suppressalis)、大型蚤(Daphnia magna)和赤子爱胜蚓(Eisenia foetida)的影响。其中, 对二化螟采用水稻稻苗饲养法, 观察接虫后6 d二化螟的存活情况, 并称取活虫体重; 对大型蚤采用水稻粉液饲喂法, 考察28 d大型蚤的存活率、繁殖数等指标; 对赤子爱胜蚓则用添加水稻秸秆的人工土壤培养法, 考察28 d内赤子爱胜蚓的存活、体重和行为情况。结果表明, 与受体对照‘广占63-4S’相比, 转BPH9和Bar基因抗褐飞虱耐除草剂水稻‘H23’对二化螟、大型蚤和赤子爱胜蚓均无显著影响, 可以认为, 短期内转BPH9和Bar基因水稻‘H23’对这3种非靶标生物无明显的生态风险。

关键词: 转基因水稻‘H23’, 二化螟, 大型蚤, 赤子爱胜蚓, 生态风险

Abstract

Aim: In order to investigate the effects of transgenic rice H23 with BPH9 and Bar genes resistant to brown planthopper and herbicide on the safety of non-target organisms, we used Chilo suppressalis, Daphnia magna and Eisenia foetida as environmental bio-indicators.

Methods: The survival and weight of C. suppressalis was examined on the 6th day after infestation at the tillering and jointing stage. Under indoor conditions, the survival rate and reproduction number of D. magna was investigated by feeding them H23 rice powder. We also measured the survival, weight and behavior of E. foetida after being fed with transgenic rice straw in artificial soil for 28 days. As control for these trials, we fed each species non-transgenic rice Guangzhan 63-4S.

Results: The survival and single weight of C. suppressalis, the survival rate and reproduction number of D. magna, the survival, weight and behavior of E. foetida had no significant difference between H23 and Guangzhan 63-4S. H23 had no significant effect on the growth of all three organisms, as compared with that of the nontransgenic control.

Conclusion: Transgenic rice H23 did not pose an ecological risk to C. suppressalis, D. magnaand E. foetida after short-term exposure.

Key words: transgenic rice H23, Chilo suppressalis, Daphnia magna, Eisenia foetida, ecological risk

李三和, 刘凯, 闸雯俊, 徐华山, 李培德, 周雷, 游艾青 (2021) 转BPH9和Bar基因抗褐飞虱耐除草剂水稻‘H23’对非靶标生物的影响. 生物多样性, 29, 488-494. DOI: 10.17520/biods.2020295.

Sanhe Li, Kai Liu, Wenjun Zha, Huashan Xu, Peide Li, Lei Zhou, Aiqing You (2021) Effects of transgenic rice H23 with BPH9 and Bar genes resistant to brown planthopper and herbicide on non-target organisms. Biodiversity Science, 29, 488-494. DOI: 10.17520/biods.2020295.

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链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2020295

https://www.biodiversity-science.net/CN/Y2021/V29/I4/488

图/表 5

表1 ‘H23’水稻对二化螟幼虫存活和生长发育的影响

Table 1 Effects of H23 on the survival rate and growth of Chilo suppressalis nymph

材料 Material	分蘖期 Tillering stage		拔节期 Jointing stage
材料 Material	存活率 Survival rate (%)	单头体重 Single weight (mg)	存活率 Survival rate (%)	单头体重 Single weight (mg)
‘H23’	82.5 ± 3.0^a	0.27 ± 0.00^a	69.2 ± 9.5^a	0.28 ± 0.06^a
‘广占63-4S’ Guangzhan 63-4S	80.6 ± 6.7^a	0.31 ± 0.06^a	71.4 ± 10.9^a	0.31 ± 0.02^a

图1 不同处理组大型蚤存活率变化趋势

Fig. 1 Survival rate of Daphnia magna in different treatment groups

图2 不同处理新生大型蚤总数。相同小写字母表示不同处理间无显著差异(P > 0.05)。

Fig. 2 Number of new born Daphnia magna in different treatments. Same lowercase letters indicate that there is no significant difference between different treatments (P > 0.05).

表2 不同处理赤子爱胜蚓死亡率

Table 2 Death rate of Eisenia foetida in different treatments

处理 Treatment	第7天 The 7th day (%)	第14天 The 14th day (%)	第28天 The 28th day (%)
发酵牛粪 + 30 mg/kg氯乙酰胺 Fermented cow dung + 30 mg/kg Chloroacetamide	33 ± 12	63 ± 12	93 ± 4.3
发酵牛粪 Fermented cow dung	0	0	0
转基因水稻?H23?秸秆 Straw of transgenic rice H23	0	0	0
非转基因水稻?广占63-4S?秸秆 Straw of non-transgenic rice Guangzhan 63-4S	0	0	0

图3 不同处理组赤子爱胜蚓体重变化。同一时间不同处理间具相同小写字母者表示无显著差异(P > 0.05)。

Fig. 3 Weight changes of Eisenia foetida in different treatment groups. Same lowercase letters indicate that there is no significant difference between different treatments in the same time (P > 0.05).

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