生物多样性

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抗褐飞虱耐除草剂转基因水稻H23对非靶标生物的影响

李三和,刘凯,闸雯俊,徐华山,李培德,周雷,游艾青   

  1. 湖北省农业科学院粮食作物研究所
  • 收稿日期:2020-07-23 修回日期:2020-09-10 出版日期:2020-09-20 发布日期:2020-09-20
  • 通讯作者: 游艾青

Effects of Transgenic rice H23 resistant to BPH and herbicide on non-target organisms

Sanhe Li,Kai Liu,Wenjun Zha,Huashan Xu,Peide Li,Lei Zhou,Aiqing You   

  1. Food Crops Institute, Hubei Academy of Agricultural Sciences
  • Received:2020-07-23 Revised:2020-09-10 Online:2020-09-20 Published:2020-09-20
  • Contact: Aiqing You

摘要: 为研究转Bph9和Bar基因抗褐飞虱耐除草剂水稻H23对非靶标生物安全性的影响,以H23的非转基因亲本改良的广占63-4S为对照,选择二化螟、大型蚤和赤子爱胜蚓为指示生物,拔节期和分蘖期接虫后6d检查二化螟的存活与发育情况,称取活虫体重;在室内条件下,用H23水稻粉液饲喂大型蚤,考察大型蚤的存活率、繁殖数指标;用加入转基因水稻秸秆的人工土壤培养赤子爱胜蚓,考察28天内赤子爱胜蚓的存活、体重和行为情况。结果表明,与改良的广占63-4S相比,转Bph9和Bar基因抗褐飞虱耐除草剂水稻H23对二化螟、大型蚤和赤子爱胜蚓无显著影响。由此可初步判定,短期内转Bph9和Bar基因水稻H23对这三种非靶标生物无生态风险。

关键词: 转基因水稻, 二化螟, 大型蚤, 赤子爱胜蚓, 存活率, 体重

Abstract: In order to investigate the effects of transgenic rice H23 resistant to BPH and herbicide on the safety of non-target organisms,Chilo suppressalis,Daphnia magna Straus and Eisenia foetida were studied as environmental bio-indicators. The survival and weight of Chilo suppressalis were examined on the 6th day after inoculation at tillering and jointing stage. Under indoor conditions,the survival rate and reproduction number of Daphnia magna were investigated by feeding H23 rice powder. Survival, weight and behavior of the Eisenia foetida, which was fed with transgenic rice straw in artificial soil for 28 days, were investigated. Non-transgenic rice Guangzhan 63-4S was used as a control in these trails. The results showed that H23 had no significant effect on the growth of both organisms,as compared with that of the nontransgenic control.Thus,we concluded that the transgenic rice H23 did not pose an ecological risk to Chilo suppressalis,Daphnia magna Straus and Eisenia foetida following short-term exposure.

Key words: transgenic rice, Chilo suppressalis, Daphnia magna Straus, Eisenia foetida, survivalrate, weight