生物多样性 ›› 2003, Vol. 11 ›› Issue (6): 480-485.DOI: 10.17520/biods.2003057

• 论文 • 上一篇    下一篇

粉拟青霉种内nrDNA ITS分析

黄勃,王成树,王滨,樊美珍,李增智   

  1. 1 (安徽农业大学微生物防治省重点实验室,合肥 230036)
    2 (中国科学院微生物研究所真菌地衣系统学重点实验室,北京 100080)
  • 收稿日期:2003-04-01 修回日期:2003-04-20 出版日期:2003-11-20 发布日期:2003-11-20
  • 通讯作者: 李增智

nrDNA ITS analysis of Paecilomyces farinosus isolates

HUANG Bo, WANG Cheng-Shu, WANG Bin, FAN Mei-Zhen, LI Zeng-Zhi   

  1. 1 Provincial Key Laboratory of Microbial Control of Anhui , Anhui Agricultural University , Hefei 230036
    2 Systematic Mycology & Lichenology Laboratory , Institute of Microbiology , Chinese Academy of Sciences ,Beijing 100080
  • Received:2003-04-01 Revised:2003-04-20 Online:2003-11-20 Published:2003-11-20
  • Contact: LI Zeng-Zhi

摘要: 通过对20株粉拟青霉(Paecilomyces farinosus)ITS1-5.8S-ITS2 (rDNA)区域序列测定,确定了粉拟青霉ITS序列,而韩国学者测定的粉拟青霉ITS序列应为细脚拟青霉(P. tenuipes)的序列。序列比较发现, 韩国2株未定名的拟青霉(Paecilomyces spp.)菌株(KACC40219、KACC40221)应为粉拟青霉。基于本研究构建的邻接树推断,粉拟青霉的有性型可能是一种虫草。粉拟青霉的起源应为单源的。不同的粉拟青霉菌株的ITS序列具有多态性,源于同一地区的菌株的ITS变异也较大。ITS序列的证据表明,粉拟青霉菌株间的差异与地理来源及寄主均无相关性。

AbstractnrDNA ITS(ITS1-5.8S-ITS2)  regions of 20 isolates of Paecilomyces farinosus were sequenced,  and its correct ITS sequence  was obtained. The results showed that the ITS of P. farinosus reported by Korean researchers was actually that of P. tenuipes. Two unidentified Korean isolates of Paecilomyces (KACC40219 and KACC40221) were identified as P. farinosus based on molecular data. The neighbour-joining tree showed that P. farinosus is monophyletic, and the teleomorph of P. farinosus was tentatively identified as a Cordyceps species.Sequence polymorphisms of the nrDNA ITS region exist among different isolates  of P. farinosus with the same geographic origins. The molecular data proved that genetic diversity of different isolates of P. farinosus is not related to geographic origins and hosts.