关键词: 16S rDNA, 古菌, 原始土壤, 非高温泉古菌, 免培养法

AbstractBy means of statistic analysis on the dynamic state of the enrichment and declination of 59 local populations in 12 surviving species in Klugieae from China ,the population extinction rate is calculated using the formula of R ( %/ 20 years) = (1 - Wi/ Wi - 1) ×100. During the period of 120 years from 1870 to 1990 ,the population extinction rate of species in Klugieae sharply increased while their habitats became severely fragmented. The population extinction rates of species in different groups are correlated with their phylogeny. The primitive groups have higher population extinction rate than the derivative groups. The endemic groups , especially the endemic genera are inclined to suffer higher extinction than the widely distributed genera.