生物多样性 ›› 2010, Vol. 18 ›› Issue (2): 182-187.DOI: 10.3724/SP.J.1003.2010.195

• 论文 • 上一篇    下一篇

春兰根中可分泌吲哚乙酸的内生细菌多样性

刘琳1, 孙磊1,*(), 张瑞英1, 姚娜2, 李潞滨2,*   

  1. 1河北大学生命科学学院, 河北省微生物多样性研究与应用实验室, 河北保定 071002
    2中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091
  • 收稿日期:2009-09-15 接受日期:2009-12-16 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 孙磊,李潞滨
  • 作者简介:sunlei1018@126.com
    *E-mail:lilubin@126.com;
  • 基金资助:
    国家“十一五”科技支撑计划课题“主要商品花卉优质高产新品种选育”之专题“优质高产热带兰新品种选育”(2006BAD01A18);“948”项目“花卉新品种创制技术引进与创新”(2009-4-C08);河北省自然科学基金项目(C2009000180);. 河北大学引进人才专项基金(2006-088);海南省重大科技研发专项“热带花卉产业可持续发展关键技术研究”课题(080102)

Diversity of IAA-producing endophytic bacteria isolated from the roots of Cymbidium goeringii

Liu Lin1, Sun Lei1,*(), Zhang Ruiying1, Yao Na2, Li Lubin2,*   

  1. 1College of Life Sciences, Hebei University, Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, Hebei 071002
    2Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091
  • Received:2009-09-15 Accepted:2009-12-16 Online:2010-03-20 Published:2010-03-20
  • Contact: Sun Lei,Li Lubin

摘要:

植物内生细菌可通过分泌吲哚乙酸(Indole-3-acetic acid, IAA)等方式促进植物生长。本研究以温室盆栽春兰(Cymbidium goeringii)为材料, 采用分离培养方法对春兰根中可分泌IAA的内生细菌多样性进行了研究。从春兰根组织中共分离纯化得到了256株内生细菌, 其中57株具有分泌IAA的能力, 占总菌数的22.3%。根据ARDRA(amplified ribosomal DNA restriction analysis)及16S rDNA系统发育分析结果, 将57株内生细菌划分为25个组, 分属于6大类群, 分别为变形菌门的α-变形菌纲(35.1%)、γ-变形菌纲(14.0%)和β-变形菌纲(8.8%)、厚壁菌门(33.3%)、放线菌门(7.0%)及拟杆菌门(1.8%)。其中变形菌门的α-变形菌纲和厚壁菌门为优势类群, 类芽孢杆菌属(Paenibacillus)为优势菌属, 且为高产IAA的主体菌属。另外, 测序结果显示有4个菌株的序列与已知细菌的最高序列相似性低于97.0%, 可能为潜在的新种或新属。研究结果表明春兰根中分泌IAA的内生细菌具有丰富的多样性。这一结果可为研究和开发植物促生细菌提供基础资料。

关键词: Cymbidium goeringii, 植物促生细菌, ARDRA, 16S rDNA

Abstract

Endophytic bacteria can directly stimulate plant growth in several ways, including production of plant hormones such as indole-3-acetic acid (IAA). We investigated the diversity of IAA-producing endophytic bacteria in the roots of Cymbidium goeringii using culture-dependent approaches. A total of 57 IAA-producing strains were obtained from 256 strains isolated from the interior roots of C. goeringii. Based on amplified ribosomal DNA restriction analyses (ARDRA) and 16S rDNA sequences, these 57 strains were divided into 25 groups. The IAA-producing endophytic bacteria were grouped into six phyla, i.e., Alphaproteobacteria (35.1%), Firmicutes (33.3%), Gammaproteobacteria (14.0%), Betaproteobacteria (8.8%), Actinobacteria (7.0%) and Bacteroidetes (1.8%). The dominant groups were Alphaproteobacteria and Firmicutes, and the dominant genus, Paenibacillus, produced relatively high levels of IAA. In addition, four potential novel taxonomic units were found. Our results revealed diverse and abundant communities of IAA-producing endophytic bacteria in the roots of C. goeringii and highlight the microbial resources represented by plant growth-promoting bacteria.

Key words: Cymbidium goeringii, plant growth-promoting bacteria, ARDRA, 16S rDNA