Biodiversity Science ›› 2016, Vol. 24 ›› Issue (3): 332-340.doi: 10.17520/biods.2015288

• Orginal Article • Previous Article     Next Article

Insect community diversity in transgenic Bt cotton in saline and dry soils

Junyu Luo, Shuai Zhang, Xiangzhen Zhu, Chunyi Wang, Limin Lü, Chunhua Li, Jinjie Cui*()   

  1. Institute of Cotton Research of Chinese Academy of Agricultural Sciences /State Key Laboratory of Cotton Biology, Anyang, Henan 455000
  • Received:2015-10-22 Accepted:2016-01-10 Online:2016-04-05
  • Cui Jinjie E-mail:cuijinjie@126.com

Cotton is one kind of economic crop that can tolerate high drought and salinity, and it has been planted increasingly in coastal saline alkaline soils and dry soils since China’s Yellow River and the Yangtze River cotton area dropped due to soil salinization and drought. In this paper, using no-transgenic cotton (CCRI 49 cotton) as control, insect community diversity in transgenic Bt cotton (CCRI 79 cotton) fields planted in coastal alkaline soils of Dongying City, Shandong Province and mildly saline and semi dry soils of Zaoqiang County, Hebei Province were investigated in 2013 and 2014. Results showed that, in the two case of spray and no-spray, the total number of individuals of insect communities and pest sub-communities in transgenic Bt cotton fields were lower than those in non-transgenic cotton, and these insect communities and pest sub-communities differed significantly. The insect diversity index and evenness index (of the insect community and pest sub-community) were higher in transgenic Bt cotton fields than those in non-transgenic cotton, but the dominant concentration index was lower than that of non-transgenic cotton. Under spraying, the total number of individuals within the insect community, pest sub-community and enemy sub-community were lower than those not sprayed fields for both transgenic Bt cotton and non-transgenic cotton. The diversity index and evenness index were lower in sprayed fields than those in no-spraying fields, but the dominant index was higher in the no-spraying cotton fields; however there were no significant difference between the sprayed and no-spraying cotton fields for both transgenic and no-transgenic cottons. Nevertherless there was lower biodiversity and simpler ecosystem than non-saline-dry soils.

Key words: saline alkaline soil, dry soil, transgenic Bt cotton, insect community

Table 1

Variance analysis of cotton field insect communities of two cotton varieties in 2013 and 2014 (F value)"

Table 2

Total individual of insect community in saline alkali soil cotton fields in 2013 and 2014 at Zaoqiang and Dongying (means ± SD)"

试验地点
Experimental site
年份
Year
营养层
Nutrition layer
施药 Spraying 不施药 No-spraying
中棉所49 CCRI 49 中棉所79 CCRI 79 中棉所49 CCRI 49 中棉所79 CCRI 79
枣强
Zaoqiang
2013 昆虫群落
Insect community
13,033.3±1,353.5 a* 10,144.6±1,122.9 a 44,117.3±1,036.7 b 40,525.2±1,346.3 b
害虫亚群落
Pest sub-community
12,747.9±1,255.8 a* 9,942.9±1,146.1 a 43,751.1±1,043.1 a 40,242.7±1,323.2 b
天敌亚群落
Enemy sub-community
285.4±37.6 a 201.7±24.2 a 366.3±13.5 a 282.5±28.4 a
2014 昆虫群落
Insect community
3,522.7±166.8 a* 2,596.3±152.2 b 28,557.7±1,090.6 a 27,490.3±1,125.7 a
害虫亚群落
Pest sub-community
3,178.3±139.7 a* 2,346.7±153.1 b 27,902.3±1,046.4 a 26,453.3±1,028.5 a
天敌亚群落
Enemy sub-community
344.3±27.6 a 249.7±21.9 a 655.3±53.0 a 837.0±116.6 a
东营
Dongying
2013 昆虫群落
Insect community
17,709.0±1,668.4 a* 15,966.3±1,861.2 a 34,317.3±2,020.9 a 20,707.3±2,461.4 b
害虫亚群落
Pest sub-community
17,595.7±1,671.2 a* 15,861.7±1,955.3 a 33,975.0±2,013.9 a 16,930.0±1,359.5 b
天敌亚群落
Enemy sub-community
113.3±13.5 a 159.7±23.1 a 342.3±60.1 a 293.3±29.7 a
2014 昆虫群落
Insect community
4,829.1±3,089.4 a* 3,707.1±1,379.6 b 24,952.2±1,498.5 a 18,995.3±1,468.3 b
害虫亚群落
Pest sub-community
4,698.9±2,997.4 a* 3,650.2±1,394.8 b 24,577.3±1,411.7 a 18,724.2±1,372.8 b
天敌亚群落
Enemy sub-community
130.3±94.4 a 56.9±15.4 a 374.9±86.8 a 271.1±107.9 a

Table 3

Diversity indexes of insect community in saline alkali soil cotton fields in 2013 and 2014 at Zaoqiang and Dongying (means ± SD)"

试验地点
Experimental site
年份
Year
营养层
Nutrition layer
施药 Spraying 不施药 No-spraying
中棉所49 CCRI 49 中棉所79 CCRI 79 中棉所49 CCRI 49 中棉所79 CCRI 79
枣强
Zaoqiang
2013 昆虫群落
Insect community
0.20±0.02 a* 0.23±0.01 a* 0.50±0.02 a 0.51±0.04 a
害虫亚群落
Pest sub-community
0.19±0.01 a* 0.21±0.01 a* 0.46±0.03 a 0.47±0.04 a
天敌亚群落
Enemy sub-community
0.62±0.03 a 0.63±0.03 a 0.41±0.05 a 0.43±0.01 a
2014 昆虫群落
Insect community
0.42±0.00 a 0.45±0.02 a 0.73±0.03 a 0.76±0.02 a
害虫亚群落
Pest sub-community
0.34±0.01 a 0.37±0.02 a 0.60±0.03 a 0.63±0.01 a
天敌亚群落
Enemy sub-community
0.53±0.01 a 0.55±0.07 a 0.60±0.01 a 0.76±0.03 a
东营
Dongying
2013 昆虫群落
Insect community
0.15±0.02 a 0.18±0.07 a 0.26±0.09 a 0.32±0.03 a
害虫亚群落
Pest sub-community
0.13±0.03 a 0.16±0.07 a 0.23±0.07 a 0.24±0.07 a
天敌亚群落
Enemy sub-community
0.40±0.03 a 0.45±0.05 a 0.37±0.03 a 0.44±0.03 a
2014 昆虫群落
Insect community
0.18±0.01 a 0.20±0.04 a 0.30±0.06 a 0.27±0.11 a
害虫亚群落
Pest sub-community
0.15±0.00 a 0.17±0.04 a 0.22±0.07 a 0.23±0.12 a
天敌亚群落
Enemy sub-community
0.34±0.12 a 0.40±0.03 a 0.39±0.04 a 0.41±0.09 a

Table 4

Evenness indexes of insect community in saline alkali soil cotton fields in 2013 and 2014 at Zaoqiang and Dongying (means ± SD)"

试验地点
Experimental site
年份
Year
营养层
Nutrition layer
施药 Spraying 不施药 No-spraying
中棉所49 CCRI 49 中棉所79 CCRI 79 中棉所49 CCRI 49 中棉所79 CCRI 79
枣强
Zaoqiang
2013 昆虫群落
Insect community
0.04±0.01 a* 0.05±0.00 a* 0.13±0.00 a 0.13±0.01 a
害虫亚群落
Pest sub-community
0.04±0.01 a* 0.04±0.01 a* 0.12±0.01 a 0.12±0.01 a
天敌亚群落
Enemy sub-community
0.18±0.01 a 0.19±0.01 a 0.23±0.01 a 0.24±0.01 a
2014 昆虫群落
Insect community
0.08±0.01 a 0.09±0.00 a 0.20±0.01 a 0.22±0.01 a
害虫亚群落
Pest sub-community
0.09±0.01 a 0.09±0.00 a 0.16±0.01 a 0.19±0.00 a
天敌亚群落
Enemy sub-community
0.21±0.01 a 0.22±0.01 a 0.25±0.01 a 0.26±0.02 a
东营
Dongying
2013 昆虫群落
Insect community
0.04±0.01 a 0.05±0.02 a 0.06±0.02 a 0.08±0.01 a
害虫亚群落
Pest sub-community
0.03±0.01 a 0.04±0.02 a 0.05±0.02 a 0.06±0.02 a
天敌亚群落
Enemy sub-community
0.14±0.01 a 0.19±0.01 a 0.18±0.02 a 0.22±0.03 a
2014 昆虫群落
Insect community
0.03±0.00 a 0.05±0.01 a 0.08±0.02 a 0.09±0.03 a
害虫亚群落
Pest sub-community
0.02±0.00 a 0.04±0.01 a 0.07±0.02 a 0.08±0.03 a
天敌亚群落
Enemy sub-community
0.17±0.04 a 0.14±0.02 a 0.20±0.04 a 0.24±0.04 a

Table 5

Dominant concentration indexes of insect community in saline alkali soilcotton fields in 2013 and 2014 at Zaoqiang and Dongying (means ± SD)"

试验地点
Experimental site
年份
Year
营养层
Nutrition layer
施药 Spraying 不施药 No-spraying
中棉所49 CCRI 49 中棉所79 CCRI 79 中棉所49 CCRI 49 中棉所79 CCRI 79
枣强
Zaoqiang
2013 昆虫群落
Insect community
0.80±0.02 a* 0.75±0.01 a* 0.36±0.02 a 0.36±0.04 a
害虫亚群落
Pest sub-community
0.81±0.02 a* 0.76±0.01 a* 0.38±0.02 a 0.37±0.04 a
天敌亚群落
Enemy sub-community
0.31±0.02 a* 0.31±0.02 a* 0.44±0.02 a 0.46±0.02 a
2014 昆虫群落
Insect community
0.78±0.01 a 0.53±0.03 a 0.35±0.02 a 0.25±0.01 a
害虫亚群落
Pest sub-community
0.85±0.01 a 0.57±0.03 a 0.42±0.03 a 0.30±0.00 a
天敌亚群落
Enemy sub-community
0.35±0.02 a 0.20±0.02 a 0.30±0.04 a 0.40±0.06 a
东营
Dongying
2013 昆虫群落
Insect community
0.87±0.04 a 0.78±0.12 a 0.69±0.15 a 0.57±0.08 a
害虫亚群落
Pest sub-community
0.88±0.05 a 0.79±0.12 a 0.70±0.14 a 0.69±0.12 a
天敌亚群落
Enemy sub-community
0.50±0.05 a 0.44±0.03 a 0.56±0.02 a 0.47±0.02 a
2014 昆虫群落
Insect community
0.89±0.01 a 0.79±0.06 a 0.70±0.07 a 0.67±0.20 a
害虫亚群落
Pest sub-community
0.92±0.01 a 0.81±0.06 a 0.74±0.08 a 0.70±0.21 a
天敌亚群落
Enemy sub-community
0.58±0.03 a 0.52±0.15 a 0.53±0.07 a 0.49±0.12 a
1 Ashraf M (2002) Salt tolerance of cotton: some new advances. Critical Reviews in Plant Sciences, 21, 1-30.
2 Bai LX, Xu NY, Zou K, Xiao LB, Liu YQ, Sun HJ (2007) Fluctuation of resistance level to cotton bollworm (Helicoverpa armgera) of Bt transgenic cotton planted in different ecological regions. Jiangsu Journal of Agricultural Sciences, 23, 299-305.(in Chinese with English abstract)
[柏立新, 许乃银, 邹奎, 肖留斌, 刘艳青, 孙厚俊 (2007) 不同生态区域转基因抗虫棉抗棉铃虫水平波动度研究. 江苏农业学报, 23, 299-305.]
3 Barrett-Lennard EG (2003) The interaction between waterlogging and salinity in higher plants: causes, consequences and implications. Plant and Soil, 253, 35-54.
4 Chen DH, Ye GY, Yang CQ, Chen Y, Wu Y (2005) The effect of high temperature on the insecticidal properties of Bt cotton. Environmental and Experimental Botany, 53, 333-342.
5 Chen WQ (2006) Current situation and development strategy of plant protection science in China and overseas. In: Proceedings of Academic Annual Conference of China Society of Plant Protection in 2006. China Agricultural Science and Technology Publishing House, Beijing.(in Chinese)
[陈万权 (2006) 国内外植物保护科技现状与发展战略. 见: 中国植物保护学会2006学术年会论文集. 中国农业科学技术出版社, 北京.]
6 Chen Y, Wen YJ, Chen Y, John TC, Zhang X, Wang YH, William AP, Chen DH (2012a) Effects of extreme air temperature and humidity on the insecticidal expression level of Bt cotton. Journal of Integrative Agriculture, 11, 1836-1844.
7 Chen Y, Chen Y, Wen YJ, Zhang X, Chen DH (2012b) The effects of the relative humidity on the insecticidal expression level of Bt cotton during bolling period under high temperature. Field Crops Research, 137, 141-147.
8 Coviella CE, Stipanovic RD, Trumble JT (2002) Plant allocation to defensive compounds: interactions between elevated CO2 and nitrogen in transgenic cotton plants. Journal of Experimental Botany, 53, 323-331.
9 Cui JJ, Xia JY (2000) Studies on the components of diversity of the community in transgenic Bt cotton. Acta Ecologica Sinica, 20, 824-829.(in Chinese with English abstract)
[崔金杰, 夏敬源 (2000) 转Bt基因棉田昆虫群落多样性及其影响因素研究. 生态学报, 20, 824-829.]
10 Deng SD, Xu J, Zhang QW, Zhou SW, Xu GJ (2003) Effect of transgenic Bt cotton on population dynamics of the non-target pests and natural enemies of pests. Acta Entomologica Sinica, 46, 1-5.(in Chinese with English abstract)
[邓曙东, 徐静, 张青文, 周世文, 徐冠军 (2003) 转Bt基因棉对非靶标害虫及害虫天敌种群动态的影响. 昆虫学报, 46, 1-5.]
11 Dong HZ, Xin CS, Tang W, Li WJ, Zhang DM, Wen SM (2006) Seasonal changes of salinity and nutrients in the coastal saline soil in Dongying, Shandong, and their effects on cotton yield. Cotton Science, 18, 362-366.(in Chinese with English abstract)
[董合忠, 辛承松, 唐薇, 李维江, 张冬梅, 温四民 (2006) 山东东营滨海盐渍棉田盐分与养分的季节性变化及对棉花产量的影响. 棉花学报, 18, 362-366.]
12 Head G, Moar W, Eubanks M, Freeman B, Ruberson J, Hagerty A, Turnipseed S (2005) A multiyear, large-scale comparison of arthropod population on commercially managed Bt and non-Bt cotton fileds. Environmental Entomology, 34, 1257-1266.
13 Huang J, Hu R, Pray C, Qiao F, Rozelle S (2003) Biotechnology as an alternative to chemical pesticides: a case study of Bt cotton in China. Agricultural Economics, 29, 55-67.
14 Kahlown MA, Azam M (2002) Individual and combined effect of waterlogging and salinity on crop yields in the Indus basin. Irrigation and Drainage, 51, 329-338.
15 Kennedy D (2003) Breakthrough of the year. Science, 302, 2033.
16 Li JG, Pu LJ, Zhu M, Zhang RS (2012) The present situation and hot issues in the salt-affected soil research. Acta Geographica Sinica, 67, 1233-1245.(in Chinese with English abstract)
[李建国, 濮励杰, 朱明, 张润森 (2012) 土壤盐渍化研究现状及未来研究热点. 地理学报, 67, 1233-1245.]
17 Liu WX, Wan FH, Guo JY (2002) Structure and seasonal dynamics of arthropods in transgenic Bt cotton field. Acta Ecologica Sinica, 22, 729-735.(in Chinese with English abstract)
[刘万学, 万方浩, 郭建英 (2002) 转Bt基因棉田节肢动物群落营养层及优势功能团的组成与变化. 生态学报, 22, 729-735.]
18 Luo Z, Dong HZ, Li WJ, Zhao M, Zhu YQ (2008) Individual and combined effects of salinity and waterlogging on Cry1Ac expression and insecticidal efficacy of Bt cotton. Crop Protection, 27, 1485-1490.
19 Martins CM, Beyene G, Hofs JL, Krüger K, Van der Vyver C, Schlüter U, Kunert KJ (2008) Effect of water-deficit stress on cotton plants expressing the Bacillus thuringiensis toxin. Annals of Applied Biology, 152, 255-262.
20 Mehmood YZ, Farooqi KB, Anjum MB, Ahmad M (2012) Impact of Bt cotton varieties on productivity: evidence from District Vehari, Pakistan. Journal of Agriculture Social Sciences, 8, 109-111.
21 Meloni DA, Oliva MA, Martinez CA, Cambraia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environmental and Experimental Botany, 49, 69-76.
22 Norbert F, Tchouaffe T (2007) Strategies to reduce the impact of salt on crops (rice, cotton and chili) production: a case study of the tsunami affected area of India. Desalination, 206, 524-530.
23 Pettigrew WT, Adamczyk JJ (2006) Nitrogen fertility and planting date effects on lint yield and Cry1Ac (Bt) endotoxin production. Agronomy Journal, 98, 691-697.
24 Sun CG, Zhang QW, Xu J, Wang YX, Liu JL (2003) Effects of transgenic Bt cotton and transgenic Bt+CpTI cotton on population dynamics of main cotton pests and their natural enemies. Acta Entomologica Sinica, 46, 705-712.(in Chinese with English abstract)
[孙长贵, 张青文, 徐静, 王因霞, 刘俊丽 (2003) 转Bt基因棉和转Bt + CpTI双价基因棉对棉田主要害虫及其天敌种群动态的影响. 昆虫学报, 46, 705-712.]
25 Sun HL (1995) Put resources and environment protection in development. Journal of Natural Resources, 10, 199-202.(in Chinese with English abstract)
[孙鸿烈 (1995) 寓资源环境保护于发展之中. 自然资源学报, 10, 199-202.]
26 Sun LZ, Liu SY, Xu JL, Chen KZ, Ai HJ (2011) The whole seedling technology of cotton planting in Zaoqiang County. Journal of Modern Agricultural Science and Technology, 73, 83.(in Chinese)
[孙良忠, 刘素云, 许金楼, 陈克正, 艾红菊 (2011) 枣强县棉花一播全苗技术. 现代农业科技, 73, 83.]
27 Wang LM, Wang JB, Shen FF, Zhang XK, Liu RZ (2001) Influences of waterlogging and drought on different transgenic Bt cotton cultivars. Cotton Science, 13, 87-90.(in Chinese with English abstract)
[王留明, 王家宝, 沈法富, 张学坤, 刘任重 (2001) 渍涝与干旱对不同转Bt基因抗虫棉的影响. 棉花学报, 13, 87-90.]
28 Wang XY, Zou YD, Meng QL, Zhang BW, Dai HM (2005) Effects of herbicides on diversity indices of cotton field arthropod community. Chinese Journal of Applied Ecology, 16, 514-518.(in Chinese with English abstract)
[王向阳, 邹运鼎, 孟庆雷, 章炳旺, 代海敏 (2005) 两种除草剂对棉田节肢动物群落多样性指数的影响. 应用生态学报, 16, 514-518.]
29 Wu HC, Cheng XN, Wei CS, Zou YD (2004) Effects of imidacloprid on arthropod community structure in tobacco field. Chinese Journal of Applied Ecology, 15, 95-98.(in Chinese with English abstract)
[巫厚长, 程遐年, 魏重生, 邹运鼎 (2004) 吡虫啉对烟田节肢动物群落的影响研究. 应用生态学报, 15, 95-98.]
30 Xu LR, Li HG, Xu CM (2002) Study on structure of insect community in Bt transgenic cotton field. Shandong Agricultural Sciences, (3), 13-17.(in Chinese with English abstract)
[许立瑞, 李洪刚, 徐春明 (2002) 转Bt基因抗虫棉田昆虫群落结构的研究. 山东农业科学, (3), 13-17.]
31 Xu Y (2010) The Ecological Impacts of Bt Cotton on Target and Non-target Insects in Xinjiang Cotton-growing Region. PhD dissertation, Zhejiang University, Hangzhou.(in Chinese with English abstract)
[徐遥 (2010) 新疆棉区Bt棉花对靶标与非靶标昆虫的生态效应. 博士学位论文, 浙江大学, 杭州.]
32 Yao DX, Zhang XY, Zhao XH, Liu CL, Wang CC, Zhang ZH, Zhang CJ, Wei Q, Wang QH, Yan H, Li FG, Su Z (2011) Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.). Genomics, 98, 47-55.
33 Zhang YJ, Wu KM, Peng YF, Guo YY (2002) The ecological risks of genetically engineered plants. Acta Ecologica Sinica, 22, 1951-1959.(in Chinese with English abstract)
[张永军, 吴孔明, 彭于发, 郭予元 (2002) 转基因植物的生态风险. 生态学报, 22, 1951-1959.]
34 Zhao KF (1993) Salt Resistance Physiology of Plant. China Science and Technology Press, Beijing.(in Chinese)
[赵可夫 (1993) 植物抗盐生理. 中国科技出版社, 北京.]
35 Zhao ZM, Guo YQ (1990) Principle and Methods of Community Ecology. Chongqing Branch of Scientific and Technical Documents Publishing House, Chongqing.(in Chinese)
[赵志模, 郭依泉 (1990) 群落生态学原理与方法. 科学技术文献出版社重庆分社, 重庆.]
36 Zhou HX, Guo JY, Wan FH (2004) Effect of transgenic Cry1Ac + CpTI cotton SGK321 on population dynamics of pests and their natural enemies. Acta Entomologica Sinica, 47, 538-542.(in Chinese with English abstract)
[周洪旭, 郭建英, 万方浩 (2004) 转Cry1Ac + CpTI基因棉对棉田害虫及其天敌种群动态的影响. 昆虫学报, 47, 538-542.]
37 Zou YD, Bi SD, Zhou XZ, Li L, Gao CQ, Cheng C (2003) Dynamics of the pest and natural enemy communities in peach orchards. Chinese Journal of Applied Ecology, 14, 717-720.(in Chinese with English abstract)
[邹运鼎, 毕守东, 周夏芝, 李磊, 高彩球, 程成 (2003) 桃园害虫及天敌群落动态研究. 应用生态学报, 14, 717-720.]
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