Biodiversity Science ›› 2012, Vol. 20 ›› Issue (4): 451-459.doi: 10.3724/SP.J.1003.2012.09221

• Original Papers • Previous Article     Next Article

Genetic variation in rusty-necklaced partridge (Alectoris magna) detected by mitochondrial DNA

Rong Zhou1, Jiaqi Li2, You Li3, Naifa Liu2*, Fengjie Fang2, Limin Shi2, Ying Wang2   

  1. 1College of Earth and Environmental Science, Lanzhou University, Lanzhou 730000, China

    2School of Life Sciences, Lanzhou University, Lanzhou 730000, China

    3School of Earth and Environmental Science, University of Adelaide, Adelaide, SA 5005, Australia
  • Received:2011-12-05 Revised:2012-04-10 Online:2012-09-12
  • Naifa Liu E-mail:naifaliu@sohu.com

The rusty-necklaced partridge (Alectoris magna) is a bird species endemic to China that has been threatened by habitat fragmentation and hunting. To examine the conservation status of this species, we examined the intraspecific variation in the mitochondrial DNA (mtDNA) control region and cytochrome b 234 individuals from 12 representative populations throughout the species’ range. Forty-four haplotypes were defined by 34 variable sites with the mean haplotype diversity of 0.916 ± 0.011 and nucleotide diversity of 0.00449 ± 0.00242 in the 1,127 bp mtDNA control region. Haplotype diversity was maximized in the Jingyuan and Minhe populations (0.894 ± 0.063) and was minimized in the Haiyuan population (0.476 ± 0.155). Fourteen haplotypes were defined by 14 variable sites with a mean haplotype diversity of 0.738 ± 0.024 and a nucleotide diversity of 0.00216 ± 0.00009 in mtDNA cytochrome b.Haplotype diversity was the highest in the Gonghe population (0.763 ± 0.059) and did not differ in the Delingha population. Correlation between genetic diversity indices and sample size did not result in statistically significant differences. We found that neighbour-joining methods revealed limited phylogenetic distinction between geographically distant populations of the rusty-necklaced partridge, and provided tentative support for a single evolutionarily significant unit. AMOVA revealed that the most genetic variation was distributed within populations; However, the genetic variation among groups and populations was also statistically significant, indicating that the appropriate short-term management unit for the rusty-necklaced partridge is a the level of the local population. Therefore, edge populations with lower genetic diversity, including Delingha, Dulan, Lixian, Zhangjiachuan and Haiyuan, have higher conservation value. We recommend that strengthening protection of populations with lower genetic diversity, including Gonghe, Guide and Jingyuan, is advantageous to retain the general level of genetic diversity in this species.

Avise JC(1994)Molecular Markers,Natural History and Evolution.Chapman&Hall,NewYork.

Avise JC,Hamrick JL(1996)Conservation genetics: Case Histories from Nature.Chapman&Hall,NewYork.

Barratt E,Gurnell J,Malarky G,Deaville R,Bruford M(1999)Genetic structure of fragmented populations of red squirrel (Sciurus vulgaris) in the UK.Molecular Ecology,8,S55-S63.

Beebee TJC,Rowe G(translated by Zhang JL(张军丽),Liao B(廖斌),Wang SL(王胜龙))(2009)An Introduction to Molecular Ecology(分子生态学),pp.183-184.Sun Yat-sen University Press,Guangzhou.(in Chinese)

Chen Q,Chang C,Liu NF(1999)Mitochondrial DNA Introgression between Two Parapatric Species of Alectoris.Acta Zoologica Sinica,45,456-463.

Excoffier L,Laval G,Schneider S(2005)Arlequin (version 3.0): an integrated software package for population genetics data analysis.Evol Bioinform Online,1,47-50.

Excoffier L,Smouse PE,Quattro JM(1992)Analysis of Molecular Variance Inferred From Metric Distances Among DNA Haplotypes: Application to Human Mitochondrial DNA Restriction Data.Genetics,131,479-491.

Felsenstein J(1985)Confidence limits on phylogenies: an approach using the bootstrap.Evolution,39,783-791.

García-Ramos G,Kirkpatrick M(1997)Genetic models of adaptation and gene flow in peripheral populations.Evolution,51,21-28.

Garner TWJ,Pearman PB,Angelone S(2004)Genetic diversity across a vertebrate species’ range: a test of the central–peripheral hypothesis.Molecular Ecology,13,1047-1053.

Groom MJ,Meffe GK,Carroll CR(2006)Principles of conservation biology.Sinauer Associates Sunderland,Massachusetts,USA.

Hoffmann AA,Blows MW(1994)Species borders: ecological and evolutionary perspectives.Trends in Ecology & Evolution,9,223-227.

Hou P(侯鹏),Wei M(卫明),Zhang LX(张立勋),Liu NF(刘迺发)(2002)Genetic Structure of Edge Population in Przewalski’s Rock Partridge (Alectoris magna).Acta Zoologica Sinica(动物学报),48,333-338.(in Chinese with English abstract)

Houlden BA,Costello BH,Sharkey D,Fowler EV,Melzer A,Ellis W,Carrick F,Baverstock PR,Elphinstone MS(1999)Phylogeographic differentiation in the mitochondrial control region in the koala,Phascolarctos cinereus (Goldfuss 1817).Molecular Ecology,8,999-1011.

Huang ZH,Liu NF,Chen YK,Xiao YA(2009a)Genetic diversity in peripheral and central populations of Rusty-necklaced Partridge (Alectoris magna) based on mitochondrial and microsatellite DNA. Acta Zoologica Academiae Scientiarum Hungaricae,55,187-197.

Huang ZH,Liu NF,Luo SX,Long J,Xiao YA(2007)Ecological genetics of rusty-necklaced partridge (Alectoris magna): Environmental factors and population genetic variability correlations.Korean Journal of Genetics,29,115-120.

Huang ZH,Yang ZS,Zhang J,Liu NF(2009b)Introgressive hybridization and population genetic diversity between rusty-necklaced partridge and chukar partridge in northwestern China.Belgian Journal of Zoology,139,15-21.

Karron JD(1987)A comparison of levels of genetic polymorphism and self-compatibility in geographically restricted and widespread plant congeners.Evolutionary Ecology,1,47-58.

Kimura M(1968)Evolutionary rate at the molecular level.Nature,217,624-626.

Lesica P,Allendorf FW(1995)When are peripheral populations valuable for conservation? Conservation Biology,9,753-760.

Librado P,Rozas J(2009)DnaSP v5: a software for comprehensive analysis of DNA polymorphism data.Bioinformatics,25,1451-1452.

Liu NF(刘迺发)(1984) On the Taxonomic Status of Alectoris magna.Acta Zootaxonomica Sinica(动物分类学报),9,212-218.(in Chinese with English abstract)

Liu NF(1992)Ecology of Przewalski's rock partridge (Alectoris magna).Gibier Faune Sauvage,9,605-615.

Liu NF(刘迺发),Huang ZH(黄族豪),Wen LY(文陇英)(2004)Subspecies Divergence of Przewalski’s Rock Partridge (Alectoris magna) Description of a New Subspecies.Acta Zootaxonomica Sinica(动物分类学报),29,600-605. (in Chinese with English abstract)

Liu NF(刘迺发),Wang XT(王香亭),Luo WY(罗文英)Chang C(常城)(1988)Avifauna Study of the LiuPan Mountain in Ningxia.Journal of Lanzhou University (Natural Sciences)(兰州大学学报(自然科学版)),24,65-75. (in Chinese with English abstract)

Liu NF,Wen LY,Huang ZH,Hou P(2006)Introgressive Hybridization between Alectoris magna and A.chukar in the Liupan Mountain Region.Acta Zoologica Sinica,52,153-159.

Merrell DJ(1981)Ecological Genetics.Longman,London.

Moritz C(1994)Defining evolutionarily significant units for conservation.Trends in Ecology and Evolution,9,373 - 375.

Moritz C,Dowling TE,Brown WM(1987)Evolution of Animal Mitochondrial DNA: Relevance for Population Biology and Systematics.Annual Review of Ecology and Systematics,18,269-292.

Qu JY,Liu NF,Bao XK,Wang XL(2009)Phylogeography of the ring-necked pheasant (Phasianus colchicus) in China.Mol Phylogenet Evol,52,125-132.

Qu Y,Lei F(2009)Comparative phylogeography of two endemic birds of the Tibetan plateau,the white-rumped snow finch (Onychostruthus taczanowskii) and the Hume's ground tit (Pseudopodoces humilis).Mol Phylogenet Evol,51,312-326.

Qu Y,Lei F,Zhang Y,Lu X(2010)Comparative phylogeography of five avian species: implications for Pleistocene evolutionary history in the Qinghai-Tibetan plateau.Molecular Ecology,19,338-351.

Randi E,Lucchini V(1998)Organization and Evolution of the Mitochondrial DNA Control Region in the Avian Genus Alectoris.Journal of Molecular Evolution,47,449-462.

Rowe G,Beebee TJC(2003)Population on the verge of a mutational meltdown? Fitness costs of genetic load for an amphibian in the wild.Evolution,57,177-181.

Sambrook J,Fritscher E,Maniatis T(1989)Molecular Cloning: A Laboratory Manual.Cold Spring Harbor Laboratory Press,New York.

Soule M(1973)The epistasis cycle: a theory of marginal populations.Annual Review of Ecology and Systematics,4,165-187.

Thompson JD,Gibson TJ,Plewniak F,Jeanmougin F,Higgins DG(1997)The CLUSTAL_X Windows Interface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools.Nucleic Acids Research,25,4876-4882.

Wang YQ(汪永庆),Zhang ZB(张知彬),Xu LX(徐来祥)(2001)Genetic Diversity in Peripheral and Central Populations of Rat-Like Hamster (Cricetulus triton).Chinese Science Bulletin(科学通报),46,1644-1650.(in Chinese)

Wei M(卫明),Hou P(侯鹏),Huang ZH(黄族豪),Liu NF(刘迺发)(2002)Effects of Environmetal Factors on the Population Genetic Structure in Alectoris magna. Acta Ecologica Sinica(生态学报),22,528-534. (in Chinese with English abstract)

Wen LY(文陇英),Zhang LX(张立勋),Liu NF(刘迺发)(2005)Phylogenetic Relationship of Perdix dauuricae Inferred from Mitochondrial Cytochrome b Gene.Zoological Research(动物学研究),26,69-75.(in Chinese with English abstract)

Yang ZS(杨志松),Liu NF(刘迺发)(2009)Introgressive Hybridization between Two Partridges Based on Mitochondrial Cytochrome b.Journal of Chongqing Normal University (Natural Science)(重庆师范大学学报(自然科学版)),26,1-6. (in Chinese with English abstract)
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