Biodiversity Science ›› 2020, Vol. 28 ›› Issue (3): 303-313.doi: 10.17520/biods.2019194

• Original Papers • Previous Article     Next Article

The reproductive characteristics of core germplasm in a native Metasequoia glyptostroboides population

Manling Wu1, Lan Yao1, *(), Xunru Ai1, Jiang Zhu1, Qiang Zhu1, Jin Wang1, Xiao Huang1, Jianfeng Hong2   

  1. 1 School of Forestry and Horticulture, Hubei Minzu University, Enshi, Hubei 445000
    2 Administration Bureau of Xingdoushan National Nature Reserve, Enshi, Hubei 445000
  • Received:2019-06-12 Accepted:2019-08-14 Online:2020-01-14
  • Lan Yao E-mail:24752851@qq.com

In order to explore the reproductive characteristics of Metasequoia glyptostroboides, we explored a native population of original Metasequoia glyptostroboides. We investigated the core germplasm of 40 individuals of native Metasequoia glyptostroboides and collected data on habitats, parent trees and seed traits. We used field experiment to analyze reproductive characteristics. We detected differences in seed traits, germination rate and seedling growth characteristics among different parent trees using ANOVA. The effects of individual traits on seeds, germination rate and seedling growth were analyzed with correlation and linear regression models. The results showed that: (1) There were only 7 strains from 40 mother trees, the seed yield rate was 17.50%, and the difference in seed yield of 7 original mother trees was significant (P < 0.05). (2) There were significant differences in seed weight (1,000 grain), seed germination rate, seedling survival rate and base diameter growth among individuals of different parent trees (P < 0.05). (3) The average germination rate of seeds from seven mother trees was 19.73%, and the average survival rate of seedlings was 73.42% (inter-individual rates were also significantly different (P < 0.05). (4) Diameter at breast height (DBH) was negatively correlated with seed weight and germination rate (P < 0.05), while seed germination and seed weight (P < 0.05), seed germination and seedling survival (P < 0.001), and tree height and seedling survival (P < 0.05) were positively correlated. (5) The height of mother tree was positively correlated to seedling survival rate (P < 0.05), and DBH was negatively correlated to seed germination rate (P < 0.05); and (6) Seedling growth (height and base diameter) conforms with logistic growth (R 2 ≥ 0.928).

Key words: Metasequoia glyptostroboides, original population, core germplasm, Logistic growth

Table 1

Characteristics of individual traits and seed production of 40 Metasequoia glyptostroboides original mother trees"

原始编号
Mother
tree no.
包含等位
基因个数
Number of
alleles*
分布特点
Distribution
characteristics
树高
Tree
height
(m)
胸径
Diameter at breast height
(cm)
冠幅
Crown
diameter (m2)
海拔
Altitude
(m)
30 m范围内环境因素
Environmental factors
within 30 m
2017年产种量
Yield in 2017
备注
Remarks
0628 11 集群
Cluster
26 85.67 56.72 1,110 道路、水沟
Road and ditch
未产种
No
0691 12 散生
Scattered
31 59.55 56.72 1,160 荒土
Wasteland
未产种
No
断梢
Shoot break
0713 9 散生
Scattered
25 54.14 19.63 1,100 道路、河沟
Road and river ditch
未产种
No
0723 12 散生
Scattered
37 78.03 63.59 1,120 黄连
Coptis chinensis
15.21 g
0732 11 散生
Scattered
20 31.53 12.56 1,120 道路、河沟
Road and river ditch
未产种
No
0735 10 散生
Scattered
19 27.07 9.62 1,120 道路、河沟
Road and river ditch
未产种
No
0744 14 集群
Cluster
39 45.86 78.50 1,170 黄连
Coptis chinensis
未产种
No
0745 11 集群
Cluster
32 55.10 94.99 1,160 山坡、河沟
Hillside and river ditch
未产种
No
0791 10 散生
Scattered
24 63.69 113.04 1,190 水沟、油竹林
Ditch and Bambusa
surrecta
forest
未产种
No
0815 10 集群
Cluster
43 92.36 63.59 1,100 道路
Road
未产种
No
0859 13 散生
Scattered
28 61.78 28.26 1,140 苗圃地、房屋
Nursery and house
未产种
No
0861 12 散生
Scattered
26 46.50 23.75 1,140 竹林
Bamboo forest
未产种
No
0950 13 集群
Cluster
38 78.03 44.16 1,170 山林路边、荒地
Mountain roadside and
wasteland
未产种
No
0956 10 散生
Scattered
32 54.14 38.47 1,190 水沟
Ditch
未产种
No
0975 10 散生
Scattered
24 64.01 33.17 1,140 苗圃地
Nursery
未产种
No
断梢
Shoot break
0981 15 集群
Cluster
37 81.85 56.72 1,130 黄连、水沟
Coptis chinensis and ditch
未产种
No
0996 11 散生
Scattered
37 69.11 50.24 1,140 水沟
Ditch
未产种
No
断梢
Shoot break
1005 13 散生
Scattered
30 62.74 38.47 1,130 金银花、土坎
Lonicera japonica and ridge
未产种
No
1009 11 散生
Scattered
- - - - - - 已死亡
Dead
1011 10 散生
Scattered
39 76.11 56.72 1,170 水沟、道路
Ditch and road
0.36 g
2415 10 散生
Scattered
42 78.66 113.04 1,090 房屋、竹林
House and bamboo forest
未产种
No
2416 12 散生
Scattered
33 78.34 63.59 1,090 道路、牛圈
Road and bought
未产种
No
2421 12 散生
Scattered
29 84.39 188.60 1,120 道路、柳杉
Road and Cryptomeria fortunei
未产种
No
4534 8 集群
Cluster
30 76.43 44.16 1,103 307.45 g
4541 12 集群
Cluster
30 105.73 50.24 1,153 221.12 g
4546 13 散生
Scattered
30 123.25 153.86 1,036 道路、河流、农田、蚁害
Road, river, farmland and
ant harm
未产种
No
10 m处分岔为两株, 根部外露
Two branches
at 10 m, with
exposed roots
4562 14 集群
Cluster
31 74.84 38.47 1,363 河流 River 未产种
No
4566 14 集群
Cluster
32 71.97 30.66 1,323 河流 River 未产种
No
5235 14 集群
Cluster
32 56.37 33.17 998 水沟 Ditch 未产种
No
5581 12 集群
Cluster
31 35.67 33.17 1,018 水沟、农田
Ditch and farmland
未产种
No
5590 13 集群
Cluster
32 62.10 56.72 1,006 房屋、道路、农田
House, road, farmland
未产种
No
5614 15 集群
Cluster
30 41.72 23.75 1,039 未产种
No
5618 16 集群
Cluster
33 48.41 33.17 1,040 未产种
No
5637 13 集群
Cluster
31 87.90 50.24 1,019 房屋、道路、农田、蚁害
House, road, farmland
and ant harm
未产种
No
5641 14 集群
Cluster
27 57.32 44.16 1,067 水沟 Ditch 未产种
No
5685 14 集群
Cluster
36 78.66 113.04 1,073 河流 River 440.40 g
5702 14 集群
Cluster
31 52.87 23.75 1,040 道路、河流、农田
Road, river and farmland
未产种
No
5705 13 集群
Cluster
29 42.04 15.90 1,043 道路、河流、农田
Road, river and farmland
728.64 g
5716 12 集群
Cluster
29 49.04 19.63 1,061 水沟 Ditch 未产种
No
5741 15 集群
Cluster
32 72.61 50.24 1,213 河流 River 1,213.28 g

Table 2

Individual traits and reproductive characteristics of seven Metasequoia glyptostroboides original mother trees"

性状
Traits
均值 ± 标准差
Mean ± SD
最大值
Max.
最小值
Min.
变异系数
Coefficient of variation (CV, %)
F
年龄 Age (yr) 107.14 ± 35.57 160.00 75.00 33.19 -
树高 Tree height (m) 33.29 ± 3.99 39.00 29.00 11.99 -
胸径 Diameter at breast height (cm) 63.30 ± 19.97 123.25 27.07 29.96 -
冠幅 Crown diameter (m2) 52.60 ± 39.18 188.60 9.62 69.38 -
鲜果千粒重 Cone thousand-grain weight (g) 2,592.58 ± 617.62 3,798.85 1,957.90 23.82 -
种子千粒重 Seed thousand-grain weight (g) 2.01 ± 0.41 2.56 1.47 20.40 22.402***
发芽率 Sprouting percentage (%) 19.73 ± 5.12 26.67 10.56 25.95 19.144***
存活率 Survival rate (%) 73.42 ± 19.30 100.00 44.11 26.29 4.539*
基径 Base diameter (mm) 1.51 ± 0.27 1.88 1.16 17.88 10.333***
苗高 Seedling height (mm) 130.27 ± 18.80 168.60 112.43 14.13 2.645

Table 3

Correlation between maternal traits and reproductive characteristics of seven Metasequoia glyptostroboides original mother trees"

年龄
Age
树高
Tree
height
胸径
Diameter at breast height
冠幅
Crown diameter
鲜果千粒重
Cone thousand-
grain weight
种子千粒重
Seed thousand-
grain weight
发芽率
Sprouting
percentage
存活率
Survival
rate
基径
Base
diameter
苗高
Seedling
height
年龄 Age 1
树高 Tree height 0.494 1
胸径 Diameter at breast height 0.047 0.149 1
冠幅 Crown diameter 0.120 0.643 0.534 1
鲜果千粒重
Cone thousand-grain weight
-0.245 0.150 -0.221 0.543 1
种子千粒重
Seed thousand-grain weight
-0.159 0.225 -0.732 0.001 0.677 1
发芽率 Sprouting percentage -0.468 -0.533 -0.843* -0.607 0.305 0.659 1
存活率 Survival rate 0.403 0.852* 0.541 0.588 -0.213 -0.230 -0.786* 1
基径 Base diameter 0.449 0.878** 0.146 0.308 -0.186 0.148 -0.459 0.866* 1
苗高 Seedling height 0.236 0.718 0.012 0.232 0.041 0.396 -0.238 0.562 0.818* 1

Table 4

Effects of mother tree traits on seed germination and seedling growth of Metasequoia glyptostroboides original mother trees"

年龄 Age 树高 Tree height 胸径 Diameter at breast height 冠幅 Crown diameter
回归系数
Regression
coefficient
决定系数
Determination coefficient
P 回归系数
Regression
coefficient
决定系数
Determination coefficient
P 回归系数
Regression coefficient
决定系数
Determination coefficient
P 回归系数
Regression coefficient
决定系数
Determination coefficient
P
发芽率
Sprouting percentage
-0.14 0.06 > 0.05 -0.82 -1.41 > 0.05 -0.48 0.65 < 0.05 -2.89 0.24 > 0.05
存活率
Survival
rate
0.31 0.20 > 0.05 3.94 0.60 < 0.05 0.61 0.21 > 0.05 5.00 0.19 > 0.05
基径
Base
diameter
0.002 -0.15 > 0.05 0.05 0.50 < 0.05 0.003 -0.15 > 0.05 0.03 -0.11 > 0.05
苗高
Seedling
height
0.02 -0.20 > 0.05 1.41 -0.09 > 0.05 -0.37 -0.05 > 0.05 -1.45 -0.16 > 0.05

Table 5

Parameters of height-logistic-equation of different Metasequoia glyptostroboides mother trees"

母树编号
Mother
tree no.
拟合方程参数 Logistic equation parameters
生长极限
Growth limitation
(k)
待定系数
Undetermined
coefficient (a)
待定系数
Undetermined
coefficient (b)
决定系数
Determination
coefficient (R2)
检验值
Levene (F)
P
0723 481.375 4.633 0.037 0.991 1,283.823 0.001
1011 394.882 4.922 0.038 0.981 570.855 0.001
4534 251.838 4.352 0.041 0.928 142.671 0.001
4541 321.098 5.137 0.043 0.976 456.289 0.001
5685 333.246 4.585 0.038 0.953 222.561 0.001
5705 351.429 4.404 0.037 0.993 1,640.055 0.001
5741 318.744 4.126 0.036 0.937 165.176 0.001

Table 6

Phenophase and growth parameters of different Metasequoia glyptostroboides mother trees"

母树编号
Mother
tree no.
物候期参数 Phenophase parameters 生长参数 Growth parameters
线性生长始期
Early stage of
linear growth (d)
线性生长末期
Final stage of
linear growth (d)
线性生长期
Stage of linear
growth (d)
最大线性生长速率
The maximum
linear growth rate
(mm/d)
平均线性生长速率
Average linear growth rate (mm/d)
线性生长量
Quantity of linear
growth (mm)
线性生长量占总
生长量的百分率
Linear growth as a percentage of total growth (%)
0723 89.622 160.811 71.189 4.453 3.958 277.922 59.13
1011 94.868 164.184 69.316 3.751 3.335 227.985 59.37
4534 74.024 138.268 64.244 2.581 2.295 145.399 57.62
4541 88.837 150.093 61.256 3.452 3.068 185.386 58.42
5685 86.000 155.316 69.316 3.166 2.814 192.400 60.06
5705 83.432 154.622 71.189 3.251 2.890 202.898 59.29
5741 78.028 151.194 73.167 2.869 2.550 184.027 55.71

Table 7

Parameters of base diameter-logistic-equation of different Metasequoia glyptostroboides mother trees"

母树编号
Mother
tree no.
拟合方程参数 Logistic equation parameters
生长极限
Growth
limitation (k)
待定系数
Undetermined
coefficient (a)
待定系数
Undetermined
coefficient (b)
决定系数
Determination
coefficient (R2)
检验值
Levene (F)
显著水平
Significant
level (P)
0723 8.454 4.277 0.028 0.983 634.530 0.001
1011 8.097 5.496 0.038 0.993 1,491.536 0.001
4534 5.286 3.280 0.019 0.981 581.010 0.001
4541 5.318 4.152 0.030 0.987 832.258 0.001
5685 4.356 4.672 0.037 0.985 716.288 0.001
5705 3.769 3.739 0.029 0.992 1,343.069 0.001
5741 3.702 3.565 0.029 0.988 915.982 0.001

Table 8

Phenophase and base diameter parameters of different Metasequoia glyptostroboides mother trees"

母树编号
Mother
tree no.
物候期参数 Phenophase parameters 生长参数 Growth parameters
线性生长始期
Early stage of
linear growth (d)
线性生长末期
Final stage of
linear growth (d)
线性生长期
Stage of linear
growth (d)
最大线性生长速率
The maximum linear
growth rate (mm/d)
平均线性生长速率
Average linear
growth rate (mm/d)
线性生长量
Quantity of linear
growth (mm)
线性生长量占总
生长量的百分率
Linear growth as a percentage of total growth (%)
0723 105.714 199.786 94.071 0.059 0.053 4.881 63.23
1011 109.974 179.289 69.316 0.077 0.068 4.675 60.95
4534 103.316 241.947 138.632 0.025 0.022 3.052 79.07
4541 94.500 182.300 87.800 0.040 0.035 3.070 60.79
5685 90.676 161.865 71.189 0.040 0.036 2.515 58.90
5705 83.517 174.345 90.828 0.027 0.024 2.176 62.17
5741 77.517 168.345 90.828 0.027 0.024 2.137 60.88

Fig. 1

Curve fitting of seedling height-logistic-equation of different Metasequoia glyptostroboides mother trees"

Fig. 2

Curve fitting of base diameter-logistic-equation of different Metasequoia glyptostroboides mother trees"

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[2] Jun Chen, Lan Yao, Xunru Ai, Jiang Zhu, Manling Wu, Xiao Huang, Siyi Chen, Jin Wang, Qiang Zhu. (2020) Adaptive strategies of functional traits of Metasequoia glyptostroboides parent trees to changing habitats . Biodiv Sci, 28(3): 296-302.
[3] WANG Xi-Qun, MA Lu-Yi, GUO Bao-Xiang, GE Ji-Wen, FAN Shen-Hou. (2004) The conservation of Metasequoia glyptostroboides and its current problems in China . Biodiv Sci, 12(3): 377-385.
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