Biodiv Sci ›› 2009, Vol. 17 ›› Issue (3): 248-256.DOI: 10.3724/SP.J.1003.2009.08316

• Special Issue • Previous Articles     Next Articles

Single nucleotide polymorphisms in chicken genomic pre-microRNA

Liying Geng1, Chuansheng Zhang2, 3*, Lixin Du3   

  1. 1 Department of Life Science, Hebei Normal University of Science & Technology, Changli, Hebei 066600
    2 Department of Animal Science, Hebei Normal University of Science & Technology, Changli, Hebei 066600
    3 National Center for Molecular and Breeding of Animal, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193
  • Received:2008-12-04 Revised:2009-02-18 Online:2009-05-20 Published:2009-05-20
  • Contact: Chuansheng Zhang

Abstract: The level of SNPs in chicken pre-microRNAs was studied to gain insight into the potential functional significance of the SNPs located in different domains of pre-microRNA. We conducted a bioinformatic survey of chicken SNPs in 471 pre-microRNAs, together with their flanking regions. The SNP density in pre-microRNA regions was significantly lower in the flanking regions (P<0.01=. The SNPs in the Seed re-gions showed greater effects on the stability of the pre-microRNA secondary structure than those SNPs in other regions did. The SNPs in the mature microRNA regions also appeared to influence target selection of the microRNA. The pre-microRNA regions may have endured more selective pressure than their flanking re-gions in the course of molecular evolution. It is possible that SNPs located in mature regions of microRNA are capable of modifying a number of biological processes by influencing the processing and target selection of microRNAs. This may contribute to the phenotypic variability observed among chickens. The findings re-ported here may provide reference information regarding the evolutionary patterns of microRNAs in chicken and may also aid in the identification of functional SNPs in microRNAs.