Single nucleotide polymorphisms in chicken genomic pre-microRNA

doi:10.3724/SP.J.1003.2009.08316

Abstract

Abstract:

The level of SNPs in chicken pre-microRNAs was studied to gain insight into the potential functional significance of the SNPs located in different domains of pre-microRNA. We conducted a bioinformatic survey of chicken SNPs in 471 pre-microRNAs, together with their flanking regions. The SNP density in pre-microRNA regions was significantly lower in the flanking regions (P<0.01=. The SNPs in the Seed regions showed greater effects on the stability of the pre-microRNA secondary structure than those SNPs in other regions did. The SNPs in the mature microRNA regions also appeared to influence target selection of the microRNA. The pre-microRNA regions may have endured more selective pressure than their flanking regions in the course of molecular evolution. It is possible that SNPs located in mature regions of microRNA are capable of modifying a number of biological processes by influencing the processing and target selection of microRNAs. This may contribute to the phenotypic variability observed among chickens. The findings reported here may provide reference information regarding the evolutionary patterns of microRNAs in chicken and may also aid in the identification of functional SNPs in microRNAs.

Key words: microRNA, bioinformatics, single nucleotide polymorphism

Liying Geng, Chuansheng Zhang, Lixin Du. Single nucleotide polymorphisms in chicken genomic pre-microRNA[J]. Biodiv Sci, 2009, 17(3): 248-256.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2009.08316

https://www.biodiversity-science.net/EN/Y2009/V17/I3/248

Figures/Tables 6

References 20

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定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	自由能改变值
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	ΔΔG kcal/mol
Loop	gga-mir-1558	rs14268121	GCCCT/-	CGCTGCTCCAGAGCCCGTTATACTCAGCAGGACAAGAGGGGAC[AGGC/-]A GGGCTGCTCACTGCTGTGATGGGAGCTCTGGGATGCG	1.9
Loop	gga-mir-133b	rs16341692	A/G	CTCTGCTCTGGCTGGTCAAACGGAACCAAGCCCGTCTTC[T/C]TCGGAGGTT TGGTCCCCTTCAACCAGCTATAGCAGTGTTGAAAA	1.4
Loop	gga-mir-124a	rs14237202	-/A	AGGCTCTGCCTCTCCGTGTTCACAGCGGACCTTGATTTAA[A/-]TCATACAA TTAAGGCACGCGGTGAATGCCAAGAGCGGATCCTCCAGGCGGCATT	0.1
Loop	gga-mir-1560	rs14968200	G/A	TCCTTAGTCACTGCGTGGGGGTGGCGGCGCGAGCAGAGAGGCGCTGCC[G/A ]TGTCCAAAGCAGCATCTCTGGACGCGCTCGTTCCTTCTGCGTGGTCCGCTT GGA	0
Loop	gga-mir-1660	rs16284368	T/C	CAAGTAAACATAAAGGCATAGTATAC[G/A]ATAGGCC TGTGTTTTTGTGCA TATTTG	0
Loop	gga-mir-1816	rs16057773	A/G	GGATGGTGAAATGATCTGAAGCTTGTAGGTTTTGTGGTTTTGTTTGACGAT T[A/G]CATGTTAAATTTAATCATCTCAACATGCATCCATCAGTGTTTTACTAATCT	0
MIR^Δseed	gga-mir-1550- 5p	rs14981477	-/G	CCGACCTGTGCAGGCTTGGGCTGATGGGGG[G/-]TGTAGTGCAGCAGCTATTTATCATGCTTCACTTACACTGCTCACTGGTCTAAGCTGCACCACCCGGG	4.0
MIR^Δseed	gga-mir-1712*	rs15403608	T/C	GCCCTTCTGGCTCCAGTCTTAACT[A/G]CAGGCAATGCTGACATTCTCCTTCAGTTATCAGTGGAGTTTGGAGTGC	3.8
MIR^Δseed	gga-mir-1675	rs16643637	T/C	GCCTTCCCGCTTGGCCCCGCTCCCGCTCAGGGCGGTGTCAGGCGGCGCCGCCGGACGCCATCTTG[A/G]CTGTGGGCAGCTCGGCTTCAGGTCGGCAGAGAGC	2.9
MIR^Δseed	gga-mir-455-5p	rs15031616	-/G	TCCCTGGTGTGAGGGTATGTGCCC[C/-]TTGGACTACATCGTGGAAGCCAGCACCATGCAGTCCATGGGCATATACACTTGCCTCAAGGT	1.0
MIR^Δseed	gga-mir-1648*	rs14281066	G/A	TGGCCGGCGGCTCGGCTCGGCTCCGCTCACACCAGGAGCAGCGGCTCGGCGTGGTGGGAGTGGA[G/A]CGGAGCTGTCCGCCGTAGCCG	0.6
MIR^Δseed	gga-mir-1596*	rs15190357	C/T	TGGACTTTGTTTCATTCTCCTTCAATCCATCTTTGTCATGATTTACAAAAAG AACAAAGGTGGCC[G/A]TGGGTGAAT GAAGCCAGGCATCCA	0.5
MIR^Δseed	gga-mir-1568	rs14511526	T/C	GCAACTGCCTTGTGATGGGTGAGTTTTCACTTTTTTTTCCTGGACTCATAG[A /G]TCTGAAGGCAGGTAGC	0.3
MIR^Δseed	gga-mir-1703	rs16718265	C/-	AGCTTGCCGCAGGATTCAGAGGCTGTA[G/-]GTCCCGTGCTTTAATTAAAAG AACGAAGCTCGTGACTTCAGCTCCAGCATCCGTGGCAGCT	0.3
Seed	gga-mir-1644	rs14076349	T/C	GTGTCCCCAGGTAGCGCTGAGCCCAGCAGGCAGTGCTGTGCCAATCTGGC TGCTC[T/C]GTTGTGCAGGGCTGTGCTCCCAGGGTGTGC	7.0
Seed	gga-mir-1658*	rs16681031	C/G	CCCCCTTCCTGAATATACCACCCCCAGGAGTTCTGCCATACCAGTGTGTGT TTCTCAGAGCT[G/C]TGGGTTGGTGTTGATGGGGAGCTGGGG	6.2
Seed	gga-mir-1648*	rs14281065	T/-	TGGCCGGCGGCTCGGCTCGGCTCCGCTCACACCAGGAGCAGCGGCTCGGC GTGGTGGGAG[T/-]GGAGCGGAGCTGTCCGCCGTAGCCG	4.4
Seed	gga-mir-1658	rs16681033	-/G	CCCCCTTCCTGAATATACCA[G/-]CCCCCAGGAGTTCTGCCATACCAGTGTG TGTTTCTCAGAGCTGTGGGTTGGTGTTGATGGGGAGCTGGGG	4.2
Seed	gga-mir-1614	rs15172520	G/A	GCTGGAGTTGGGTGGCATGGCAGACTCACCCTGCAGCTCTGACACAGGGC AGG[G/A]AGGAACTGCCAGCAGACTCAGCTCCAGC	4.0
Seed	gga-mir-1568	rs14511527	A/G	GCAACTGCCTTGTGATGGGTGAGTTTTCACTTTTTTTTCCTGGACTCA[C/T]A GATCTGAAGGCAGGTAGC	1.5
Seed	gga-mir-1657	rs14934924	G/A	GAGAAGTATATGTGTATACATCAACACATACTTATATTTGTATATGGATTTA CATATAT[G/A]TGTATTGTTGGTGTGTGA TACGATATTCCATATC	0.3
Seed	gga-mir-1658*	rs16681032	C/T	CCCCCTTCCTGAATATACCACCCCCAGGAGTTCTGCCATACCAGTGTGTGTT TCTCAGA[G/A]CTGTGGGTTGGTGTTGATGGGGAGCTGGGG	0
MIR*	gga-mir-1658	rs16681031	C/G	CCCCCTTCCTGAATATACCACCCCCAGGAGTTCTGCCATACCAGTGTGTGTT TCTCAGAGCT[G/C]TGGGTTGGTGTTGATGGGGAGCTGGGG	6.2
MIR*	gga-mir-1652	rs16744833	C/G	GGGGAAGGCCCGTGGGGATGCTCCTACCCGGG[G/C]CGCGGGGCTGCTGTCCCCACCATCCCTGTGTCCCCTCGGTGGCAGCAGGACTCTGCCATCCCC	5.4
MIR*	gga-mir-1648	rs14281065	T/-	TGGCCGGCGGCTCGGCTCGGCTCCGCTCACACCAGGAGCAGCGGCTCGGC GTGGTGGGAG[T/-]GGAGCGGAGCTGTCCGCCGTAGCCG	4.4
MIR*	gga-mir-1658*	rs16681033	-/G	CCCCCTTCCTGAATTACCA[G/-]CCCCCAGG AGTTCTGCCATACCAGTGTGT GTTTCTCAGAGCTGTGGGTTGGTGTTGATGGGGAGCTGGGG	4.2
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	自由能改变值
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	ΔΔG (kcal/mol)
MIR*	gga-mir-1550-3p	rs14981477	-/G	CCAGACCTGTGCAGGCTTGGGCTGATGGGGG[G/-]TGTAGTGCAGCAGCTAT TTATCATGCTTCACTTACACTGCTCACTGGTCTAAGCTGCACCACCCGGG	4.0
MIR*	gga-mir-1614	rs15172520	G/A	GCTGGAGTTGGGTGGCATGGCAGACTCACCCTGCAGCTCTGACACAGGGC AGG[G/A]AGGAACTGCCAGCAGACTCAGCTCCAGC	4.0
MIR*	gga-mir-1656	rs13942534	-/T	TCTCTGTCACCAGCGGGCATTGGCATCACTGGCATGGCTGTG[T/-]ATGTCA TGAGTGCTGGCAGCAGGA	3.8
MIR*	gga-mir-1712	rs15403608	T/C	GCCCTTCTGGCTCCAGTCTTAACT[A/G]CAGGCAATGCTGACATTCTCCTTCAGTTATCAGTGGAGTTTGGAGTGC	3.8
MIR*	gga-mir-449	rs14734667	G/A	CTGTGTGCGGTGGGGTGGCAGTGTATGTTAGCTGGTTGAAACTCTTGACAT CAGCTAACA[C/T]GCAGTTGCTAACCTGCTCCACATAC	3.3
MIR*	gga-mir-1659	rs15847005	G/A	CACTAGTTTCATATCCAGAGGAAGACTTCATTTTATTTAATGTT[C/T]TCATT TCACTGAATAGGGCAGAATGAAGGAGTCCCTGCCTGTGGTGAGTGACAGTG	2.6
MIR*	gga-mir-1683	rs15262856	A/C	GCTGTGCCAGGCTCCACTGGCGATGCTT[T/G]TTGTCACCTGGATGTGAGATGGTCAGTTCTGGGACAGTCCAGCATCTTTCTGGTTTATGCTGTGTCAGC	2.4
MIR*	gga-mir-1610	rs16628188	T/C	CATGGCGCCGCTCG[A/G]GCCCGCAGAGGCCGCCGGGAGCTGCAGTGCGCGCGGCATGGCTTGTGGTGGAACGGGCGGCTCCCCTGTCGTG	1.6
MIR*	gga-mir-1783	rs14039125	CT/-	CTGAAGTGTTATGAAATCGTGCTCTGAACGAGCTGAGAGGCATGCTACTTTCTGATCATTGCATCCAG[CT/-]CTCTCATTGCTCATTTCATAACTCCTTTCAG	1.4
MIR*	gga-mir-138-1	rs15960797	C/A	CCCTGCCGGGTGCCGTGCAGCAGCTGGTGTTGTGAATCAGGCCGTCACCAGTCGGAGAACGGCTACTTCACAACACCAG[G/T]GTGGCACTGCACCACA	1.3
MIR*	gga-mir-1605	rs15600725	G/A	AAGAGAAGTTCACAGAGGGTTTCATGTGTATCTCTTGAAGATGCTGAGTATGAGAGAAGCATATGAAAG[C/T]GCTTCTGTGATTTTTCTT	1.3
MIR*	gga-mir-455-3p	rs15031616	-/G	TCCCTGGTGTGAGGGTATGTGCCC[C/-]TTGGACTACATCGTGGAAGCCAGCACCATGCAGTCCATGGGCATATACACTTGCCTCAAGGT	1.0
MIR*	gga-mir-1783	rs14039126	A/G	CTGAAGTGTTATGAAATCGTGCTCTGAACGAGCTGAGAGGCATGCTACTTTCTGATCATTGCATCAGCTCTCTC[A/G]TTGCTCATTTCATAACTCCTTTCAG	0.7
MIR*	gga-mir-16-1	rs15498115	T/-	GTCTGTCATACTCTAGCAGCACGTAAATATTGGTGTTAAAACTGTAAATATC TCCAGTATTA[A/-]CTGTGCTGCTGAAGTAAGGCT	0.6
MIR*	gga-mir-1648	rs14281066	G/A	TGGCCGGCGGCTCGGCTCGGCTCCGCTCACACCAGGAGCAGCGGCTCGGCGTGGTGGGAGTGGA[G/A]CGGAGCTGTCCGCCGTAGCCG	0.6
MIR*	gga-mir-1719	rs14969646	C/G	TTGAGAAGTACTTGTTTGTACCAAAAGATGATGAGTTGCTGATGTGCTTTGTCGAGGTAACGCT[G/C]TTTCCTGGGTGCAGTATCGCTTAA	0.6
MIR*	gga-mir-1596	rs15190357	C/T	TGGACTTTGTTTCATTCTCCTTCAATCCATCTTTGTCATGATTTACAAAAAG AACAAAGGTGGCC[G/A]TGGGTGAATGAAGCCAGGCATCCA	0.5
MIR*	gga-mir-1558	rs14268122	A/G	CGCTGCTCCGAGCCCGTTATA[T/C]TCAGCAGGACAAGAGGGGACAGGCAGGGCTGCTCACTGCTGTGATGGGAGCTCTGGGATGCG	0.2
MIR*	gga-mir-1605	rs15600727	T/C	AAGAGAAGTTCACAGAGGGTTTCATGTGTATCTCTTGAAGATGCTGATATG AGAGA[A/G]GCATATGAAAGCGCTTCTGTGATTTTTCTT	0
MIR*	gga-mir-1606	rs14431112	C/G	TGTGAGGCTGCTCTT[G/C]GGTTTCCCTCTGAAATTCACTGAAAGAGGGGGCAACAGAACAGTCCTGAACA	0
MIR*	gga-mir-1618	rs14639002	C/G	CTGCGTGCTGCATCCTGGCTCCTGG[C/G]GTCCTGCACGTGCTATTGGAGAGCCCGGAGCCAGGCTGCTGTTCTCCAGGCAG	0
MIR*	gga-mir-1630	rs16651110	T/C	TTTGGGCTTT[C/T]GTTTCTTCTTTCTAAAGGGGGAGGAAAGAGGAAACTGAAGCTGAAA	0
MIR*	gga-mir-1658	rs16681032	C/T	CCCCCTTCCTGAATATACCACCCCCAGGAGTTCTGCCATACCAGTGTGTGTTTCTCAGA[G/A]CTGTGGGTTGGTGTTGATGGGGAGCTGGGG	0
Other	gga-mir-1600	rs14726712	T/A	GCCCTTCTGGCTCCAGTCTTAACT[A/G]CAGGCAATGCTGACATTCTCCTTCAGTTATCAGTGGAGTTTGGAGTGC	4.6
Other	gga-mir-1768	rs13538425	T/G	TATCAGCTCATCAGCATGACAATCCTCTGCTGCCTTGGGAGTTGTGAGCAGAGGATTGTCGTGCTGA[T/G]GAGTTGATA	4.3
Other	gga-mir-1562	rs14653234	A/T	ATCAACAAGCGGGGTGCAGCTGCACTGTAGATGGAGGGGCTGCATGAAGGTGGATAGTTTCTGTGCCCCGTGTATGCAGTGCAGGTGCTC[T/T]CTGCACTT GGT	4.1
Other	gga-mir-1453	rs16159272	G/A	GTGCAGGCTCTCTGTGGCTCAGCCCAGCTG[T/C]GGTACTGCATGGTGCTGGGCAGCCTCTCAGTGAGCTCTGTGCT	3.8
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	自由能改变值
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	ΔΔG (kcal/mol)
Other	gga-mir-1666	rs14120863	C/G	GCCAGGAGCAAACACGA[G/C]TTAACGCCACGGGGCTGAGGCTGAGCTGCA AACTTTCACAGCTTCAGAGCGTGGCTGCAGCGCGTGCTGGC	3.5
Other	gga-mir-1704	rs14668705	C/G	GACCGA[C/G]AATTCACAGCAGATTGATGGGGCACACCAAGATCTGTGCCT GTTCCTGCTGGGATGCTCCCGTC	3.4
Other	gga-mir-1792	rs14317161	A/T	TCCAAAGCCACCTCTATGCTGCGATAGCTCAGGAACACTGCAGTCAAGAACACATACTGGAGTGTTCCTGGGGTATCACAGCGTAGGGGTGGT[T/A]TTGGA	3.2
Other	gga-mir-1812	rs16571431	A/G	CTTTATTGGGAACCTAGATATATACTGTATACTGTGTCAAGGTTTAGATGACATACTATACAGTATATATCTAGGTT[T/C]CTAATACAAG	2.9
Other	gga-mir-1608	rs16662849	A/G	TCTCATCTGGTGCCCCCGGGATGGGACAGTGGCTGCGCCCTCTGTGAAGGGAA[C/T]GGAGGGCTCAGCAGCTGTGCCCGTGCCTGGCAGAGTGGA	2.5
Other	gga-mir-1705	rs15027520	A/G	GCTGAGAGCTTTCCTCAACTGTGTGCTGATTTCATTAGACTTTTTCCTGACAAGAAATCTGGAAGTCAGCACATGCTGAGAAGA[G/A]TCTGC	2.1
Other	gga-mir-1757	rs15505339	G/C	GCTGTTTTGCCGTGGATGAACAAAACTCTTCTGGTCCGCTGTTTAGTTCAATTAGCGCTTCAGAGGCGGAACAGTAGGTTTCTGTTCTT[C/G]CAGCCAAGATTCTGT	1.8
Other	gga-mir-1749	rs15860000	C/T	GAGTGCAGCTGGAGGGCTACAGGGAGATAAGGAACTGAACTGGCTGCATCTCTGCTGCTTGGCTCTGTTCCCTATTTCC[C/T]GTCTCCTTAGCAAATCTC	1.5
Other	gga-mir-1657	rs14934923	A/G	GAGAAGTAT[A/G]TGTGTATACATCAACACATACTTATATTTGTATATGGAT TTACATATATGTGTATTGTTGGTGTGTGATACGATATTCCATATC	1.2
Other	gga-mir-1562	rs14653232	A/G	ACAACA[A/G]GCGGGGTGCAGCTGCACTGTAGATGGAGGGGCTGCATGAAGGTGGATAGTTTCTGTGCCCCGTGTATGCAGTGCAGGTGCTCACTGCACTGGT	1.1
Other	gga-mir-1704	rs14668704	C/T	GACCGAGAATTCACAGCAGATTGATGGGGCACACCAAGATCTGTGCCTGTTCCTGCTGGGATGCTCCC[G/A]TC	1.0
Other	gga-mir-1558	rs15169308	G/A	CGCTGCTCCAGAGCCCGTTATACTCAGCAGGACAAGAGGGGACAGGCAGGGCTGCTCACTGCTGTGATGGGAGCTCTGGGATG[C/T]G	0.9
Other	gga-mir-1790	rs16361960	G/A	GGCGGGGCGGGATGCCGCTGGGGC[T/C]GGGCCTCGTGCCGTGCTCTGCTGGGCCGGAGAAGGCGGGTGAGGGCGGTGGGGGAAGGGAAGCGGAGCTGTTTCTTCGCT	0.8
Other	gga-mir-1675	rs16643638	C/T	GCCTTCCC[G/A]CTTGGCCCCGCTCCCGCTCAGGGCGGTGTCAGGCGGCGCCGCCGGACGCCATCTTGACTGTGGGCAGCTCGGCTTCAGGTCGGCAGAGAGC	0.7
Other	gga-mir-551	rs16677358	A/G	CCACCAGAGC[T/G]GCCCCATGGCTCCAGAAATCAAGGGTGGGTAAGACCT TGTAGGATAACTGGCAGGCGACCCATACTTGGTTTCAGGGGCTGTGTGGGTATCG- TCAGTGGTGG	0.6
Other	gga-mir-1762	rs13608151	T/C	GGTGCTCCTCCAGCTGCAGGGCAGGAGGGAAGCAGAGCTTAGAGCTGTTTTCTACGCTCGAGCTGGCTTTGCTCAAGCCCCGTGTGCGG[A/G]GGGTTTCC	0.5
Other	gga-mir-1608	rs15961488	A/C	TCTCATCTGGTGCCCCCGGGATGGGACAGTGGCTGCGCCCTCTGTGAAGGGAATGGAGGGCTCAGCAGCTGTGCCCGTGCCTGGCAGAG[T/G]GGA	0.5
Other	gga-mir-1719	rs14969645	A/G	TTGAGAAGTACTTGTTTGTACCAAAAGATGATGAGTTGCTGATGTGCTTTGTCGAGGTAACGCTGTTTCCTGGG[T/C]GCAGTATCGCTTA	0.4
Other	gga-mir-1458	rs16654946	A/-	TTAGTTCAGCCTCA[T/-]TTTTTTTTAGAGTTCTGGAGTATCTTATTGCACAAAATATGCTGAGGAGAAATTCCTGTGATGCTCATGAGAAAAATTGTAGAGCGCTTTTACACT	0.4
Other	gga-mir-1562	rs14653233	G/A	ACAACAAGCGGG[G/A]TGCAGCTGCACTGTAGATGGAGGGGCTGCATGAAGGTGGATAGTTTCTGTGCCCCGTGTATGCAGTGCAGGTGCTCACTGCACTTGG GT	0.4
Other	gga-mir-1619	rs14280616	G/T	TTGTAAGTCCAGCAGTCTGTATTGCTACAAATGCTCTTCTCAGCGCAA[C/A] CTCAGAAGAATTCATAGCAATACGGACTGCTGGACTTCTAG	0.3
Other	gga-mir-1675	rs16643636	A/T	GCCTTCCCGCTTGGCCCCGCTCCCGCTCAGGGCGGTGTCAGGCGGCGCCG- CCGGACGCCATCTTGACTGTGGGCAGC[T/A]CGGCTTCAGGTCGGCAGAG- AGC	0.3
Other	gga-mir-1687	rs15179830	T/G	GATTTCTTTGTGTCTGTGGTCTGAGGAAATGAGCCAGCTGAGTTAGCAACTTCTTTGCTGGCTGTTTCTCAGTGACTGCAGCATAAAAA[T/G]C	0.2
Other	gga-mir-22	rs14120771	C/T	GCTCCTGGCTGCCC[G/A]GCAGCAGTTCTTCAGTGGCAAGCTTTATGTCCTTCTCTAGTAGCTAAAGCTGCCAGTTGAAGAACTGTTGAATGTAGCCACGTTC	0
Other	gga-mir-1659	rs15847007	G/A	CATAGTTTCATAT[C/T]CAGAGGAAGACTTCATTTTATTTAATGTTCTCATTTCACTGAATAGGGCAGAATGAAGGAG TCC CTGCCTGTGGTGAGTGACAGTG	0
Other	gga-mir-1590	rs15781755	A/G	TGGA[T/C]GGAAATAGGTATCTCCAGAGAACAGTTTGTCCCACTTGTTTTTGACGTCTGTCGTAGCAAGGGACAAATTGCTCTCTGGAAGTACATTTCTCCA	0

定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	自由能改变值
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	ΔΔG kcal/mol
Loop	gga-mir-1558	rs14268121	GCCCT/-	CGCTGCTCCAGAGCCCGTTATACTCAGCAGGACAAGAGGGGAC[AGGC/-]A GGGCTGCTCACTGCTGTGATGGGAGCTCTGGGATGCG	1.9
Loop	gga-mir-133b	rs16341692	A/G	CTCTGCTCTGGCTGGTCAAACGGAACCAAGCCCGTCTTC[T/C]TCGGAGGTT TGGTCCCCTTCAACCAGCTATAGCAGTGTTGAAAA	1.4
Loop	gga-mir-124a	rs14237202	-/A	AGGCTCTGCCTCTCCGTGTTCACAGCGGACCTTGATTTAA[A/-]TCATACAA TTAAGGCACGCGGTGAATGCCAAGAGCGGATCCTCCAGGCGGCATT	0.1
Loop	gga-mir-1560	rs14968200	G/A	TCCTTAGTCACTGCGTGGGGGTGGCGGCGCGAGCAGAGAGGCGCTGCC[G/A ]TGTCCAAAGCAGCATCTCTGGACGCGCTCGTTCCTTCTGCGTGGTCCGCTT GGA	0
Loop	gga-mir-1660	rs16284368	T/C	CAAGTAAACATAAAGGCATAGTATAC[G/A]ATAGGCC TGTGTTTTTGTGCA TATTTG	0
Loop	gga-mir-1816	rs16057773	A/G	GGATGGTGAAATGATCTGAAGCTTGTAGGTTTTGTGGTTTTGTTTGACGAT T[A/G]CATGTTAAATTTAATCATCTCAACATGCATCCATCAGTGTTTTACTAATCT	0
MIR^Δseed	gga-mir-1550- 5p	rs14981477	-/G	CCGACCTGTGCAGGCTTGGGCTGATGGGGG[G/-]TGTAGTGCAGCAGCTATTTATCATGCTTCACTTACACTGCTCACTGGTCTAAGCTGCACCACCCGGG	4.0
MIR^Δseed	gga-mir-1712*	rs15403608	T/C	GCCCTTCTGGCTCCAGTCTTAACT[A/G]CAGGCAATGCTGACATTCTCCTTCAGTTATCAGTGGAGTTTGGAGTGC	3.8
MIR^Δseed	gga-mir-1675	rs16643637	T/C	GCCTTCCCGCTTGGCCCCGCTCCCGCTCAGGGCGGTGTCAGGCGGCGCCGCCGGACGCCATCTTG[A/G]CTGTGGGCAGCTCGGCTTCAGGTCGGCAGAGAGC	2.9
MIR^Δseed	gga-mir-455-5p	rs15031616	-/G	TCCCTGGTGTGAGGGTATGTGCCC[C/-]TTGGACTACATCGTGGAAGCCAGCACCATGCAGTCCATGGGCATATACACTTGCCTCAAGGT	1.0
MIR^Δseed	gga-mir-1648*	rs14281066	G/A	TGGCCGGCGGCTCGGCTCGGCTCCGCTCACACCAGGAGCAGCGGCTCGGCGTGGTGGGAGTGGA[G/A]CGGAGCTGTCCGCCGTAGCCG	0.6
MIR^Δseed	gga-mir-1596*	rs15190357	C/T	TGGACTTTGTTTCATTCTCCTTCAATCCATCTTTGTCATGATTTACAAAAAG AACAAAGGTGGCC[G/A]TGGGTGAAT GAAGCCAGGCATCCA	0.5
MIR^Δseed	gga-mir-1568	rs14511526	T/C	GCAACTGCCTTGTGATGGGTGAGTTTTCACTTTTTTTTCCTGGACTCATAG[A /G]TCTGAAGGCAGGTAGC	0.3
MIR^Δseed	gga-mir-1703	rs16718265	C/-	AGCTTGCCGCAGGATTCAGAGGCTGTA[G/-]GTCCCGTGCTTTAATTAAAAG AACGAAGCTCGTGACTTCAGCTCCAGCATCCGTGGCAGCT	0.3
Seed	gga-mir-1644	rs14076349	T/C	GTGTCCCCAGGTAGCGCTGAGCCCAGCAGGCAGTGCTGTGCCAATCTGGC TGCTC[T/C]GTTGTGCAGGGCTGTGCTCCCAGGGTGTGC	7.0
Seed	gga-mir-1658*	rs16681031	C/G	CCCCCTTCCTGAATATACCACCCCCAGGAGTTCTGCCATACCAGTGTGTGT TTCTCAGAGCT[G/C]TGGGTTGGTGTTGATGGGGAGCTGGGG	6.2
Seed	gga-mir-1648*	rs14281065	T/-	TGGCCGGCGGCTCGGCTCGGCTCCGCTCACACCAGGAGCAGCGGCTCGGC GTGGTGGGAG[T/-]GGAGCGGAGCTGTCCGCCGTAGCCG	4.4
Seed	gga-mir-1658	rs16681033	-/G	CCCCCTTCCTGAATATACCA[G/-]CCCCCAGGAGTTCTGCCATACCAGTGTG TGTTTCTCAGAGCTGTGGGTTGGTGTTGATGGGGAGCTGGGG	4.2
Seed	gga-mir-1614	rs15172520	G/A	GCTGGAGTTGGGTGGCATGGCAGACTCACCCTGCAGCTCTGACACAGGGC AGG[G/A]AGGAACTGCCAGCAGACTCAGCTCCAGC	4.0
Seed	gga-mir-1568	rs14511527	A/G	GCAACTGCCTTGTGATGGGTGAGTTTTCACTTTTTTTTCCTGGACTCA[C/T]A GATCTGAAGGCAGGTAGC	1.5
Seed	gga-mir-1657	rs14934924	G/A	GAGAAGTATATGTGTATACATCAACACATACTTATATTTGTATATGGATTTA CATATAT[G/A]TGTATTGTTGGTGTGTGA TACGATATTCCATATC	0.3
Seed	gga-mir-1658*	rs16681032	C/T	CCCCCTTCCTGAATATACCACCCCCAGGAGTTCTGCCATACCAGTGTGTGTT TCTCAGA[G/A]CTGTGGGTTGGTGTTGATGGGGAGCTGGGG	0
MIR*	gga-mir-1658	rs16681031	C/G	CCCCCTTCCTGAATATACCACCCCCAGGAGTTCTGCCATACCAGTGTGTGTT TCTCAGAGCT[G/C]TGGGTTGGTGTTGATGGGGAGCTGGGG	6.2
MIR*	gga-mir-1652	rs16744833	C/G	GGGGAAGGCCCGTGGGGATGCTCCTACCCGGG[G/C]CGCGGGGCTGCTGTCCCCACCATCCCTGTGTCCCCTCGGTGGCAGCAGGACTCTGCCATCCCC	5.4
MIR*	gga-mir-1648	rs14281065	T/-	TGGCCGGCGGCTCGGCTCGGCTCCGCTCACACCAGGAGCAGCGGCTCGGC GTGGTGGGAG[T/-]GGAGCGGAGCTGTCCGCCGTAGCCG	4.4
MIR*	gga-mir-1658*	rs16681033	-/G	CCCCCTTCCTGAATTACCA[G/-]CCCCCAGG AGTTCTGCCATACCAGTGTGT GTTTCTCAGAGCTGTGGGTTGGTGTTGATGGGGAGCTGGGG	4.2
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	自由能改变值
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	ΔΔG (kcal/mol)
MIR*	gga-mir-1550-3p	rs14981477	-/G	CCAGACCTGTGCAGGCTTGGGCTGATGGGGG[G/-]TGTAGTGCAGCAGCTAT TTATCATGCTTCACTTACACTGCTCACTGGTCTAAGCTGCACCACCCGGG	4.0
MIR*	gga-mir-1614	rs15172520	G/A	GCTGGAGTTGGGTGGCATGGCAGACTCACCCTGCAGCTCTGACACAGGGC AGG[G/A]AGGAACTGCCAGCAGACTCAGCTCCAGC	4.0
MIR*	gga-mir-1656	rs13942534	-/T	TCTCTGTCACCAGCGGGCATTGGCATCACTGGCATGGCTGTG[T/-]ATGTCA TGAGTGCTGGCAGCAGGA	3.8
MIR*	gga-mir-1712	rs15403608	T/C	GCCCTTCTGGCTCCAGTCTTAACT[A/G]CAGGCAATGCTGACATTCTCCTTCAGTTATCAGTGGAGTTTGGAGTGC	3.8
MIR*	gga-mir-449	rs14734667	G/A	CTGTGTGCGGTGGGGTGGCAGTGTATGTTAGCTGGTTGAAACTCTTGACAT CAGCTAACA[C/T]GCAGTTGCTAACCTGCTCCACATAC	3.3
MIR*	gga-mir-1659	rs15847005	G/A	CACTAGTTTCATATCCAGAGGAAGACTTCATTTTATTTAATGTT[C/T]TCATT TCACTGAATAGGGCAGAATGAAGGAGTCCCTGCCTGTGGTGAGTGACAGTG	2.6
MIR*	gga-mir-1683	rs15262856	A/C	GCTGTGCCAGGCTCCACTGGCGATGCTT[T/G]TTGTCACCTGGATGTGAGATGGTCAGTTCTGGGACAGTCCAGCATCTTTCTGGTTTATGCTGTGTCAGC	2.4
MIR*	gga-mir-1610	rs16628188	T/C	CATGGCGCCGCTCG[A/G]GCCCGCAGAGGCCGCCGGGAGCTGCAGTGCGCGCGGCATGGCTTGTGGTGGAACGGGCGGCTCCCCTGTCGTG	1.6
MIR*	gga-mir-1783	rs14039125	CT/-	CTGAAGTGTTATGAAATCGTGCTCTGAACGAGCTGAGAGGCATGCTACTTTCTGATCATTGCATCCAG[CT/-]CTCTCATTGCTCATTTCATAACTCCTTTCAG	1.4
MIR*	gga-mir-138-1	rs15960797	C/A	CCCTGCCGGGTGCCGTGCAGCAGCTGGTGTTGTGAATCAGGCCGTCACCAGTCGGAGAACGGCTACTTCACAACACCAG[G/T]GTGGCACTGCACCACA	1.3
MIR*	gga-mir-1605	rs15600725	G/A	AAGAGAAGTTCACAGAGGGTTTCATGTGTATCTCTTGAAGATGCTGAGTATGAGAGAAGCATATGAAAG[C/T]GCTTCTGTGATTTTTCTT	1.3
MIR*	gga-mir-455-3p	rs15031616	-/G	TCCCTGGTGTGAGGGTATGTGCCC[C/-]TTGGACTACATCGTGGAAGCCAGCACCATGCAGTCCATGGGCATATACACTTGCCTCAAGGT	1.0
MIR*	gga-mir-1783	rs14039126	A/G	CTGAAGTGTTATGAAATCGTGCTCTGAACGAGCTGAGAGGCATGCTACTTTCTGATCATTGCATCAGCTCTCTC[A/G]TTGCTCATTTCATAACTCCTTTCAG	0.7
MIR*	gga-mir-16-1	rs15498115	T/-	GTCTGTCATACTCTAGCAGCACGTAAATATTGGTGTTAAAACTGTAAATATC TCCAGTATTA[A/-]CTGTGCTGCTGAAGTAAGGCT	0.6
MIR*	gga-mir-1648	rs14281066	G/A	TGGCCGGCGGCTCGGCTCGGCTCCGCTCACACCAGGAGCAGCGGCTCGGCGTGGTGGGAGTGGA[G/A]CGGAGCTGTCCGCCGTAGCCG	0.6
MIR*	gga-mir-1719	rs14969646	C/G	TTGAGAAGTACTTGTTTGTACCAAAAGATGATGAGTTGCTGATGTGCTTTGTCGAGGTAACGCT[G/C]TTTCCTGGGTGCAGTATCGCTTAA	0.6
MIR*	gga-mir-1596	rs15190357	C/T	TGGACTTTGTTTCATTCTCCTTCAATCCATCTTTGTCATGATTTACAAAAAG AACAAAGGTGGCC[G/A]TGGGTGAATGAAGCCAGGCATCCA	0.5
MIR*	gga-mir-1558	rs14268122	A/G	CGCTGCTCCGAGCCCGTTATA[T/C]TCAGCAGGACAAGAGGGGACAGGCAGGGCTGCTCACTGCTGTGATGGGAGCTCTGGGATGCG	0.2
MIR*	gga-mir-1605	rs15600727	T/C	AAGAGAAGTTCACAGAGGGTTTCATGTGTATCTCTTGAAGATGCTGATATG AGAGA[A/G]GCATATGAAAGCGCTTCTGTGATTTTTCTT	0
MIR*	gga-mir-1606	rs14431112	C/G	TGTGAGGCTGCTCTT[G/C]GGTTTCCCTCTGAAATTCACTGAAAGAGGGGGCAACAGAACAGTCCTGAACA	0
MIR*	gga-mir-1618	rs14639002	C/G	CTGCGTGCTGCATCCTGGCTCCTGG[C/G]GTCCTGCACGTGCTATTGGAGAGCCCGGAGCCAGGCTGCTGTTCTCCAGGCAG	0
MIR*	gga-mir-1630	rs16651110	T/C	TTTGGGCTTT[C/T]GTTTCTTCTTTCTAAAGGGGGAGGAAAGAGGAAACTGAAGCTGAAA	0
MIR*	gga-mir-1658	rs16681032	C/T	CCCCCTTCCTGAATATACCACCCCCAGGAGTTCTGCCATACCAGTGTGTGTTTCTCAGA[G/A]CTGTGGGTTGGTGTTGATGGGGAGCTGGGG	0
Other	gga-mir-1600	rs14726712	T/A	GCCCTTCTGGCTCCAGTCTTAACT[A/G]CAGGCAATGCTGACATTCTCCTTCAGTTATCAGTGGAGTTTGGAGTGC	4.6
Other	gga-mir-1768	rs13538425	T/G	TATCAGCTCATCAGCATGACAATCCTCTGCTGCCTTGGGAGTTGTGAGCAGAGGATTGTCGTGCTGA[T/G]GAGTTGATA	4.3
Other	gga-mir-1562	rs14653234	A/T	ATCAACAAGCGGGGTGCAGCTGCACTGTAGATGGAGGGGCTGCATGAAGGTGGATAGTTTCTGTGCCCCGTGTATGCAGTGCAGGTGCTC[T/T]CTGCACTT GGT	4.1
Other	gga-mir-1453	rs16159272	G/A	GTGCAGGCTCTCTGTGGCTCAGCCCAGCTG[T/C]GGTACTGCATGGTGCTGGGCAGCCTCTCAGTGAGCTCTGTGCT	3.8
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	自由能改变值
定位 Location	基因 microRNA	SNP 登录号 dbSNP	变异 Variation	Pre-microRNA序列变异 Variation of the pre-microRNA sequence	ΔΔG (kcal/mol)
Other	gga-mir-1666	rs14120863	C/G	GCCAGGAGCAAACACGA[G/C]TTAACGCCACGGGGCTGAGGCTGAGCTGCA AACTTTCACAGCTTCAGAGCGTGGCTGCAGCGCGTGCTGGC	3.5
Other	gga-mir-1704	rs14668705	C/G	GACCGA[C/G]AATTCACAGCAGATTGATGGGGCACACCAAGATCTGTGCCT GTTCCTGCTGGGATGCTCCCGTC	3.4
Other	gga-mir-1792	rs14317161	A/T	TCCAAAGCCACCTCTATGCTGCGATAGCTCAGGAACACTGCAGTCAAGAACACATACTGGAGTGTTCCTGGGGTATCACAGCGTAGGGGTGGT[T/A]TTGGA	3.2
Other	gga-mir-1812	rs16571431	A/G	CTTTATTGGGAACCTAGATATATACTGTATACTGTGTCAAGGTTTAGATGACATACTATACAGTATATATCTAGGTT[T/C]CTAATACAAG	2.9
Other	gga-mir-1608	rs16662849	A/G	TCTCATCTGGTGCCCCCGGGATGGGACAGTGGCTGCGCCCTCTGTGAAGGGAA[C/T]GGAGGGCTCAGCAGCTGTGCCCGTGCCTGGCAGAGTGGA	2.5
Other	gga-mir-1705	rs15027520	A/G	GCTGAGAGCTTTCCTCAACTGTGTGCTGATTTCATTAGACTTTTTCCTGACAAGAAATCTGGAAGTCAGCACATGCTGAGAAGA[G/A]TCTGC	2.1
Other	gga-mir-1757	rs15505339	G/C	GCTGTTTTGCCGTGGATGAACAAAACTCTTCTGGTCCGCTGTTTAGTTCAATTAGCGCTTCAGAGGCGGAACAGTAGGTTTCTGTTCTT[C/G]CAGCCAAGATTCTGT	1.8
Other	gga-mir-1749	rs15860000	C/T	GAGTGCAGCTGGAGGGCTACAGGGAGATAAGGAACTGAACTGGCTGCATCTCTGCTGCTTGGCTCTGTTCCCTATTTCC[C/T]GTCTCCTTAGCAAATCTC	1.5
Other	gga-mir-1657	rs14934923	A/G	GAGAAGTAT[A/G]TGTGTATACATCAACACATACTTATATTTGTATATGGAT TTACATATATGTGTATTGTTGGTGTGTGATACGATATTCCATATC	1.2
Other	gga-mir-1562	rs14653232	A/G	ACAACA[A/G]GCGGGGTGCAGCTGCACTGTAGATGGAGGGGCTGCATGAAGGTGGATAGTTTCTGTGCCCCGTGTATGCAGTGCAGGTGCTCACTGCACTGGT	1.1
Other	gga-mir-1704	rs14668704	C/T	GACCGAGAATTCACAGCAGATTGATGGGGCACACCAAGATCTGTGCCTGTTCCTGCTGGGATGCTCCC[G/A]TC	1.0
Other	gga-mir-1558	rs15169308	G/A	CGCTGCTCCAGAGCCCGTTATACTCAGCAGGACAAGAGGGGACAGGCAGGGCTGCTCACTGCTGTGATGGGAGCTCTGGGATG[C/T]G	0.9
Other	gga-mir-1790	rs16361960	G/A	GGCGGGGCGGGATGCCGCTGGGGC[T/C]GGGCCTCGTGCCGTGCTCTGCTGGGCCGGAGAAGGCGGGTGAGGGCGGTGGGGGAAGGGAAGCGGAGCTGTTTCTTCGCT	0.8
Other	gga-mir-1675	rs16643638	C/T	GCCTTCCC[G/A]CTTGGCCCCGCTCCCGCTCAGGGCGGTGTCAGGCGGCGCCGCCGGACGCCATCTTGACTGTGGGCAGCTCGGCTTCAGGTCGGCAGAGAGC	0.7
Other	gga-mir-551	rs16677358	A/G	CCACCAGAGC[T/G]GCCCCATGGCTCCAGAAATCAAGGGTGGGTAAGACCT TGTAGGATAACTGGCAGGCGACCCATACTTGGTTTCAGGGGCTGTGTGGGTATCG- TCAGTGGTGG	0.6
Other	gga-mir-1762	rs13608151	T/C	GGTGCTCCTCCAGCTGCAGGGCAGGAGGGAAGCAGAGCTTAGAGCTGTTTTCTACGCTCGAGCTGGCTTTGCTCAAGCCCCGTGTGCGG[A/G]GGGTTTCC	0.5
Other	gga-mir-1608	rs15961488	A/C	TCTCATCTGGTGCCCCCGGGATGGGACAGTGGCTGCGCCCTCTGTGAAGGGAATGGAGGGCTCAGCAGCTGTGCCCGTGCCTGGCAGAG[T/G]GGA	0.5
Other	gga-mir-1719	rs14969645	A/G	TTGAGAAGTACTTGTTTGTACCAAAAGATGATGAGTTGCTGATGTGCTTTGTCGAGGTAACGCTGTTTCCTGGG[T/C]GCAGTATCGCTTA	0.4
Other	gga-mir-1458	rs16654946	A/-	TTAGTTCAGCCTCA[T/-]TTTTTTTTAGAGTTCTGGAGTATCTTATTGCACAAAATATGCTGAGGAGAAATTCCTGTGATGCTCATGAGAAAAATTGTAGAGCGCTTTTACACT	0.4
Other	gga-mir-1562	rs14653233	G/A	ACAACAAGCGGG[G/A]TGCAGCTGCACTGTAGATGGAGGGGCTGCATGAAGGTGGATAGTTTCTGTGCCCCGTGTATGCAGTGCAGGTGCTCACTGCACTTGG GT	0.4
Other	gga-mir-1619	rs14280616	G/T	TTGTAAGTCCAGCAGTCTGTATTGCTACAAATGCTCTTCTCAGCGCAA[C/A] CTCAGAAGAATTCATAGCAATACGGACTGCTGGACTTCTAG	0.3
Other	gga-mir-1675	rs16643636	A/T	GCCTTCCCGCTTGGCCCCGCTCCCGCTCAGGGCGGTGTCAGGCGGCGCCG- CCGGACGCCATCTTGACTGTGGGCAGC[T/A]CGGCTTCAGGTCGGCAGAG- AGC	0.3
Other	gga-mir-1687	rs15179830	T/G	GATTTCTTTGTGTCTGTGGTCTGAGGAAATGAGCCAGCTGAGTTAGCAACTTCTTTGCTGGCTGTTTCTCAGTGACTGCAGCATAAAAA[T/G]C	0.2
Other	gga-mir-22	rs14120771	C/T	GCTCCTGGCTGCCC[G/A]GCAGCAGTTCTTCAGTGGCAAGCTTTATGTCCTTCTCTAGTAGCTAAAGCTGCCAGTTGAAGAACTGTTGAATGTAGCCACGTTC	0
Other	gga-mir-1659	rs15847007	G/A	CATAGTTTCATAT[C/T]CAGAGGAAGACTTCATTTTATTTAATGTTCTCATTTCACTGAATAGGGCAGAATGAAGGAG TCC CTGCCTGTGGTGAGTGACAGTG	0
Other	gga-mir-1590	rs15781755	A/G	TGGA[T/C]GGAAATAGGTATCTCCAGAGAACAGTTTGTCCCACTTGTTTTTGACGTCTGTCGTAGCAAGGGACAAATTGCTCTCTGGAAGTACATTTCTCCA	0

定位 Location	个数 Number	均值 ± 标准误 Mean ± SE	最小值 Minimum	最大值 Maximum
Loop	6	0.5667 ± 0.34897 ^a	0.00	1.90
MIR^Δseed	8	1.6750 ± 0.57001 ^ab	0.30	4.00
Seed	8	3.4500 ± 0.92157 ^b	0.00	7.00
MIR*	24	1.7208 ± 0.37003 ^a	0.00	6.20
Other	30	1.5667 ± 0.26916 ^a	0.00	4.60

定位 Location	个数 Number	均值 ± 标准误 Mean ± SE	最小值 Minimum	最大值 Maximum
Loop	6	0.5667 ± 0.34897 ^a	0.00	1.90
MIR^Δseed	8	1.6750 ± 0.57001 ^ab	0.30	4.00
Seed	8	3.4500 ± 0.92157 ^b	0.00	7.00
MIR*	24	1.7208 ± 0.37003 ^a	0.00	6.20
Other	30	1.5667 ± 0.26916 ^a	0.00	4.60

靶基因 Target gene	突变体 Mutation type	野生型 Wild type	吉布斯自由能改变量 ΔΔG (kcal/mol)
DOCK9		3' GCT-AC-A-T-C-A-G-GT-TCCCGTGTAT 5'(-14.60) \| \| \|\| \| \| : :\| \| \|\|\|\|\|\|\|\| 5' C-AGTGTTCTTTTTTTGTGTATGGCACATA 3'	14.60
TNRC6B		3' GCT-ACATCAGGTTCCCGTGTAT 5'(-19.36) \| \| \|\|\| \|\|:\| \|\|\|\|\|\|\|\| 5' C-AGTGT-GTTC-T-GGCACATG 3'	19.36
CPEB1	3' GCTACATCAGGTTCCCCGTGTAT 5' (-26.26) :\|:\|\|\|\|\|\|:: \|\|\|\|\|\|\|\| 5' TGGTGTAGTTTT-T-GGCACATA 3'	3' GCTACATCAGGTTCCCGTGTAT 5'(-26.94) :\|:\|\|\|\|\|\|:: \|\|\|\|\|\|\|\| 5' TGGTGTAGTTT-TTGGCACATA 3'	0.68
KCNJ2	3' GCTAC - A - TCA - G - GT -TC - CCCGTGTAT 5'(-18.7) \| \|\|\| \| \|\|\| : \| \|\| \|\|\|\|\|\|\|\| 5' C-ATGTCTAAGTATGAACAGAAGGCACATA 3'	3' GCTACAT-C-AG-GTTCCCGTGTAT 5'(-16.67) \| \| \|\|\| \| \| \|\| \|\|\|\|\|\|\|\| 5' CTAAGTATGAACAGAA-GGCACATA 3'	2.03
WDR22	3' GC-TACATCA-GGTTCCCCGTGTAT 5'(-17.3) \|\| \|\|\|\| \|\| ::\|\| \|\|\|\|\|\|\|\| 5' CGTAGTGTGTATTAA-AGGCACATA 3'	3' GC-T-ACATCA-GGTTCCCGTGTAT 5'(-18.37) \|\| \| \|\|\|\|\| ::\|\| \|\|\|\|\|\|\|\| 5' CGTAGTGT-GTATTAAAGGCACATA 3'	1.07
ADD3		3' GCTACATCAGGT - T-CCCGTGTAT 5'(-14.65) \|\| \|\| \|::\| \| \|\|\|\|\|\|\|\| 5' AGAAATATTATACACTGGCACATA 3'	14.65
PAPOLG	3' GCTACAT-CAGGTTC-C-CCGTGTAT 5'(-19.88) \| \|\|\| \|\|\| \|\| \|\|\|\|\|\|\|\| 5' AAA-GTATTTCC-AGTTTGGCACATA 3'	3' GCTACAT-CAGGTT - C-CCGTGTAT 5'(-19.49) \| \|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\| 5' AAA-GTATTTCCAGTTTGGCACATA 3'	0.39
PPP1R12A	3' GCTACATCAGGTTCCCCGTGTAT 5'(-16.71) \|\|\| \| \|\|\| \| \|\|\|\|\|\|\|\| 5' CGA-C-A-TCCTATCGGCACATA 3'	3' GCTACATCAGGTT-CCCGTGTAT 5'(-16.32) \|\|\| \| \|\|\| \| \|\|\|\|\|\|\| 5' CGA-C-A-TCCTATCGGCACATA 3'	0.39