Genetic diversity in natural populations of Sorbus pohuashanensis

doi:10.3724/SP.J.1003.2008.08201

Abstract

Abstract:

Sorbus pohuashanensis is a tree species native to northern China with high potential for ornamental use. The genetic structure and diversity of eight natural populations of Sorbus pohuashanensis were studied at ten loci encoding four isozymes using horizontal starch gel electrophoresis. Genetic diversity was high at the species level with the mean number of alleles per locus N_a= 2.2000, the percentage of polymorphic loci P = 100%, and the mean expected heterozygosity H_e= 0.4240. Values of the three parameters of H_e, N_e, and Shannon′s information index indicated a random pattern of genetic diversity among the eight populations. A low level of genetic differentiation among populations (F_st= 0.0758) and a high estimate of gene flow (N_m= 3.0472) were detected together with a high level of genetic identity (I) among the populations (from 0.8585 to 0.9872). Wright’sF-statistics analysis indicated an excess of heterozygotes both at the individual population level (F_is= -0.4180) and at the total population level (F_it= -0.3105). χ²-tests (P<0.05) indicated that 73.62% of the assayed loci departed from the Hardy-Weinberg equilibrium. The UPGMA cluster analysis suggested that the genetic distances among populations were weakly correlated with their geographic distances.

Key words: Sorbus pohuashanensis, isozyme, genetic diversity, genetic structure, genetic differentiation

Jian Zheng, Yongqi Zheng, Chuanhong Zhang, Yichen Zong, Bojing Li, Chao Wu. Genetic diversity in natural populations of Sorbus pohuashanensis[J]. Biodiv Sci, 2008, 16(6): 562-569.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2008.08201

https://www.biodiversity-science.net/EN/Y2008/V16/I6/562

Figures/Tables 7

References 29

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群体代号 Population code	采样地 Seed collection site	经纬度 Geographic location	海拔 Altitude (m)	家系数量 No. of families	每家系种子数 No. of seeds per family	样本大小 Sample size
SDL	山东崂山 Laoshan, Shandong	120°37.5′E 36°11′N	493-991	12	30	360
SDT	山东泰山 Taishan, Shandong	117°06.1′E 36°15.8′N	1,002-1,451	30	10	300
SXP	山西庞泉沟 Pangquangou, Shanxi	111°25.7′E 37°53.6′N	1,834-2,179	12	30	360
HBB	河北白石山 Baishishan, Hebei	114°41.0′E 39°13.2′N	1,654-1,931	12	30	360
HBT	河北驼梁山 Tuoliangshan, Hebei	113°49.5′E 38°45.1′N	1,564-1,971	30	8	240
HBW	河北雾灵山 Wulingshan, Hebei	117°35.0′E 40°38.0′N	809-1,847	30	8	240
HBS	河北塞罕坝 Saihanba, Hebei	117°39.0′E 42°36.0′N	1,287-1,674	30	8	240
LNL	辽宁老秃顶子 Laotudingzi, Liaoning	124°53.9′E 41°19.2′N	890-1,245	12	30	360

群体代号 Population code	采样地 Seed collection site	经纬度 Geographic location	海拔 Altitude (m)	家系数量 No. of families	每家系种子数 No. of seeds per family	样本大小 Sample size
SDL	山东崂山 Laoshan, Shandong	120°37.5′E 36°11′N	493-991	12	30	360
SDT	山东泰山 Taishan, Shandong	117°06.1′E 36°15.8′N	1,002-1,451	30	10	300
SXP	山西庞泉沟 Pangquangou, Shanxi	111°25.7′E 37°53.6′N	1,834-2,179	12	30	360
HBB	河北白石山 Baishishan, Hebei	114°41.0′E 39°13.2′N	1,654-1,931	12	30	360
HBT	河北驼梁山 Tuoliangshan, Hebei	113°49.5′E 38°45.1′N	1,564-1,971	30	8	240
HBW	河北雾灵山 Wulingshan, Hebei	117°35.0′E 40°38.0′N	809-1,847	30	8	240
HBS	河北塞罕坝 Saihanba, Hebei	117°39.0′E 42°36.0′N	1,287-1,674	30	8	240
LNL	辽宁老秃顶子 Laotudingzi, Liaoning	124°53.9′E 41°19.2′N	890-1,245	12	30	360

位点 Locus	等位基因 Allele	等位基因频率 Allele frequencies
位点 Locus	等位基因 Allele	HBW	HBS	HBT	HBB	SDL	SDT	SXP	LNL
Pgm-1	a	0.3229	0.3500	0.2497	0.1958	0.2458	0.2467	0.2917	0.4889
	b	0.4521	0.3667	0.4979	0.4181	0.4222	0.3433	0.4486	0.2278
	c	0.2550	0.2833	0.2542	0.3861	0.3319	0.4100	0.2597	0.2833
Pgm-2	a	0.6917	0.9417	0.4250	0.8194	0.1736	0.2233	0.7181	0.7514
Pgm-2	b	0.3083	0.0583	0.5750	0.1806	0.8264	0.7767	0.2819	0.2486
Pgm-3	a	0.6917	0.7396	0.5021	0.8986	0.3764	0.6167	0.9361	0.9333
Pgm-3	b	0.3083	0.2604	0.4979	0.1014	0.6236	0.3833	0.0639	0.0667
Pgi-1	a	0.0167	0.0125	0.0854	0.0181	0.0556	0.0000	0.0361	0.0153
	b	0.1437	0.2167	0.1187	0.1889	0.3889	0.2500	0.2153	0.2708
	c	0.8396	0.7708	0.7958	0.7931	0.5556	0.7500	0.7486	0.7139
Pgi-2	a	0.6854	0.9500	0.5583	0.6611	0.8333	0.9450	0.6264	0.7194
Pgi-2	b	0.3146	0.0500	0.4417	0.3389	0.1667	0.0550	0.3736	0.2806
Pgd-1	a	0.7562	0.6771	0.8729	0.9069	0.9347	0.9867	0.6917	0.9653
Pgd-1	b	0.2437	0.3229	0.1271	0.0931	0.0653	0.0133	0.3083	0.0347
Pgd-2	a	0.0375	0.1562	0.0042	0.1861	0.0000	0.2050	0.0278	0.1181
Pgd-2	b	0.9625	0.8438	0.9958	0.8139	1.0000	0.7950	0.9722	0.8819
Pgd-3	a	0.5104	0.5250	0.5104	0.5194	0.5056	0.5000	0.5000	0.5014
Pgd-3	b	0.4896	0.4750	0.4896	0.4806	0.4944	0.5000	0.5000	0.4986
Mdh-1	a	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-1	b	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-2	a	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-2	b	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000

位点 Locus	等位基因 Allele	等位基因频率 Allele frequencies
位点 Locus	等位基因 Allele	HBW	HBS	HBT	HBB	SDL	SDT	SXP	LNL
Pgm-1	a	0.3229	0.3500	0.2497	0.1958	0.2458	0.2467	0.2917	0.4889
	b	0.4521	0.3667	0.4979	0.4181	0.4222	0.3433	0.4486	0.2278
	c	0.2550	0.2833	0.2542	0.3861	0.3319	0.4100	0.2597	0.2833
Pgm-2	a	0.6917	0.9417	0.4250	0.8194	0.1736	0.2233	0.7181	0.7514
Pgm-2	b	0.3083	0.0583	0.5750	0.1806	0.8264	0.7767	0.2819	0.2486
Pgm-3	a	0.6917	0.7396	0.5021	0.8986	0.3764	0.6167	0.9361	0.9333
Pgm-3	b	0.3083	0.2604	0.4979	0.1014	0.6236	0.3833	0.0639	0.0667
Pgi-1	a	0.0167	0.0125	0.0854	0.0181	0.0556	0.0000	0.0361	0.0153
	b	0.1437	0.2167	0.1187	0.1889	0.3889	0.2500	0.2153	0.2708
	c	0.8396	0.7708	0.7958	0.7931	0.5556	0.7500	0.7486	0.7139
Pgi-2	a	0.6854	0.9500	0.5583	0.6611	0.8333	0.9450	0.6264	0.7194
Pgi-2	b	0.3146	0.0500	0.4417	0.3389	0.1667	0.0550	0.3736	0.2806
Pgd-1	a	0.7562	0.6771	0.8729	0.9069	0.9347	0.9867	0.6917	0.9653
Pgd-1	b	0.2437	0.3229	0.1271	0.0931	0.0653	0.0133	0.3083	0.0347
Pgd-2	a	0.0375	0.1562	0.0042	0.1861	0.0000	0.2050	0.0278	0.1181
Pgd-2	b	0.9625	0.8438	0.9958	0.8139	1.0000	0.7950	0.9722	0.8819
Pgd-3	a	0.5104	0.5250	0.5104	0.5194	0.5056	0.5000	0.5000	0.5014
Pgd-3	b	0.4896	0.4750	0.4896	0.4806	0.4944	0.5000	0.5000	0.4986
Mdh-1	a	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-1	b	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-2	a	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-2	b	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000

群体 Population	样本数量 Sample size	N_a	N_e	P (%)	H_o	H_e	I_s
河北雾灵山 HBW	240	2.2000	1.8068	100.00	0.5717	0.4148	0.6206
河北塞罕坝 HBS	240	2.2000	1.7510	100.00	0.5500	0.3819	0.5814
河北驼梁山 HBT	240	2.2000	1.8423	100.00	0.5771	0.4192	0.6234
河北白石山 HBB	360	2.2000	1.7854	100.00	0.5436	0.3875	0.5932
山东崂山 SDL	360	2.1000	1.7837	90.00	0.5342	0.3850	0.5824
山东泰山 SDT	300	2.1000	1.7538	100.00	0.5403	0.3810	0.5707
山西庞泉沟 SXP	360	2.2000	1.7968	100.00	0.5900	0.4017	0.6054
辽宁老秃顶子 LNL	360	2.2000	1.7159	100.00	0.5367	0.3728	0.5695
平均 Mean	308	2.1750	1.7732	98.75	0.5555	0.3930	0.5933