Genetic diversity and evolution of Fujian domestic duck breeds

doi:10.3724/SP.J.1003.2008.08179

Abstract

Abstract:

To study the genetic diversity and evolution of four breeds of Fujian domestic ducks, the 667 bp control region (D-loop) of mitochondrial DNA from 32 individual domestic ducks and eight spot-billed ducks (Anas zonorhyncha) were sequenced. The results showed that in the D-loop region of four domestic duck breeds, the content of A, G, C and T nucleotides was 25.5%, 15.2%, 33.5% and 25.8%, respectively. Eight haplotypes were found, in which Hap2 (consistent with haplotype A7 in GenBank) was dominant. The average haplotype diversity (Hd) and nucleotide diversity (Pi) of domestic ducks were 0.75202 and 0.21%, respectively. The Hdand Pi of Jinding duck was the highest among the four domestic duck breeds. Kimura 2-parameter genetic distance among the four duck breeds were 0.00094-0.00395. Combined with the sequences of mallard (A. platyrhynchos) and spot-billed ducks taken from GenBank, a total of 30 haplotypes were used in the phylogenetic analysis, and the results showed that the four domestic duck breeds originated from A. platyrhynchos.

Key words: domestic ducks, D-loop region, genetic diversity, evolution

Huifang Li, Wenjuan Xu, Wenqi Zhu, Sumei Xue, Kuanwei Chen. Genetic diversity and evolution of Fujian domestic duck breeds[J]. Biodiv Sci, 2008, 16(6): 607-612.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2008.08179

https://www.biodiversity-science.net/EN/Y2008/V16/I6/607

Figures/Tables 4

Table 1 Genetic diversity in four domestic duck breeds

品种 Breed	样本量 Sample size	单倍型数 No. of haplotypes	单倍型比例 Haplotype ratio (%)	单倍型多样度 Haplotype diversity Hd	平均核苷酸差异数Mean number of nucleotide differences K	核苷酸多样度 Nucleotide diversity Pi
莆田黑鸭 PT	8	3	37.5	0.46429	0.75000	0.00112
金定鸭 JD	8	3	37.5	0.75000	0.96429	0.00145
连城白鸭 LC	8	3	37.5	0.60714	0.78571	0.00118
山麻鸭 SM	8	2	25.0	0.42857	0.42857	0.00064

Fig. 1 A dendrogram of four domestic duck breeds of Fujian based on D-loop sequences. Population codes see Table 1.

Fig. 2 A phylogenetic tree based on D-loop sequences constructed with NJ method using Kimura’s two-parameter model

Fig. 3 Reduced median-joining networks of mtDNA D-loop haplotypes. The hollow circles represent the haplotypes of Fujian domestic ducks, and the solid circles represent the haplotypes of mallard.

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