Biodiv Sci ›› 2017, Vol. 25 ›› Issue (6): 647-653. DOI: 10.17520/biods.2017001
• Original Papers • Previous Articles Next Articles
Yanping Xie1, Jianli Zhao2, Xingfu Zhu1, Li Li1, Qingjun Li1,2,*()
Received:
2017-01-01
Accepted:
2017-06-17
Online:
2017-06-20
Published:
2017-07-10
Contact:
Li Qingjun
Yanping Xie, Jianli Zhao, Xingfu Zhu, Li Li, Qingjun Li. Asymmetric hybridization of Primula secundiflora and P. poissonii in three sympatric populations[J]. Biodiv Sci, 2017, 25(6): 647-653.
Fig. 1 Locations of the three populations from Geza Village (GZ), Potatso National Park (PTS) and Shangri-La Alpine Botanical Garden (SABG) in Shangri-La
居群 Population | 物种 Species | 个体数 No. of individuals | trnH-psbA变异位点及位置 Sequence region and position (bp) of trnH-psbA | |||
---|---|---|---|---|---|---|
36 bp | 63 bp | 103 bp | 112-386 bp | |||
海仙报春 Primula poissonii | 7 | G | C | A | 1* | |
偏花报春 P. secundiflora | 7 | A | A | T | 2* | |
格咱 Geza Village (GZ) | 杂交个体类型1 Hybrid type-1 | 6 | G | C | A | 1* |
杂交个体类型2 Hybrid type-2 | 4 | A | A | T | 2* | |
香格里拉高山植物园 Shangri-La Alpine Botanical Garden (SABG) | 杂交个体类型1 Hybrid type-1 | 2 | G | C | A | 1* |
普达措国家公园 Potatso National Park (PTS) | 杂交个体类型1 Hybrid type-1 | 15 | G | C | A | 1* |
杂交个体类型2 Hybrid type-2 | 5 | A | A | T | 2* |
Table 1 Positions at which hybrids and parents differ in the trnH-psbA sequence
居群 Population | 物种 Species | 个体数 No. of individuals | trnH-psbA变异位点及位置 Sequence region and position (bp) of trnH-psbA | |||
---|---|---|---|---|---|---|
36 bp | 63 bp | 103 bp | 112-386 bp | |||
海仙报春 Primula poissonii | 7 | G | C | A | 1* | |
偏花报春 P. secundiflora | 7 | A | A | T | 2* | |
格咱 Geza Village (GZ) | 杂交个体类型1 Hybrid type-1 | 6 | G | C | A | 1* |
杂交个体类型2 Hybrid type-2 | 4 | A | A | T | 2* | |
香格里拉高山植物园 Shangri-La Alpine Botanical Garden (SABG) | 杂交个体类型1 Hybrid type-1 | 2 | G | C | A | 1* |
普达措国家公园 Potatso National Park (PTS) | 杂交个体类型1 Hybrid type-1 | 15 | G | C | A | 1* |
杂交个体类型2 Hybrid type-2 | 5 | A | A | T | 2* |
Fig. 3 The nuclear ITS and chloroplast trnH-psbA phylogeny tree based on Bayes method. GZ, PTS and SABG are the abbreviations of populations from Geza Village, Potatso National Park and Shangri-La Alpine Botanical Garden.
来源 Source | Wald 卡方值 | 自由度 | P值 |
---|---|---|---|
Wald Chi-Square | df | P-value | |
种内 vs. 种间 Within vs. between species (WBS) | 61.110 | 1 | < 0.001 |
海仙报春 vs. 偏花报春作母本 Primula poissonii vs. P. secundiflora as mother (PSM) | 29.078 | 1 | < 0.001 |
交互作用 Interaction (WBS × PSM) | 22.074 | 1 | < 0.001 |
Table 2 Effects of cross-pollination treatments (within or between species and mother species) on seed production
来源 Source | Wald 卡方值 | 自由度 | P值 |
---|---|---|---|
Wald Chi-Square | df | P-value | |
种内 vs. 种间 Within vs. between species (WBS) | 61.110 | 1 | < 0.001 |
海仙报春 vs. 偏花报春作母本 Primula poissonii vs. P. secundiflora as mother (PSM) | 29.078 | 1 | < 0.001 |
交互作用 Interaction (WBS × PSM) | 22.074 | 1 | < 0.001 |
[32] | Richards J (2003) Primula. Timber Press Inc., Oregon. |
[33] | Runyeon-Lager H, Prentice HC (2000) Morphometric variation in a hybrid zone between the weed, Silene vulgaris, and the endemic, Silene uniflora ssp. petraea (Caryophyllaceae), on the Baltic Island of Öland. Canadian Journal of Botany, 78, 1384-1397. |
[34] | Sang T, Crawford DJ, Stuessy TF (1997) Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). American Journal of Botany, 84, 1120-1136. |
[35] | Stace CA (1975) Hybridization and the Flora of the British Isles. Academic Press, London. |
[36] | Tang XL, Liu YM, Pan HT, Ma YL, Li XY, Zhang QX (2014) Intergeneric cross-compatibility between Primula forbesii (Section Monocarpicae) and Primula saxatilis (Section Cortusoides). Acta Botanica Boreali-Occidentalia Sinica, 34, 270-275. (in Chinese with English abstract) |
[唐星林, 刘艳梅, 潘会堂, 马玉磊, 李小远, 张启翔 (2014) 小报春与岩生报春种间杂交亲和性研究. 西北植物学报, 34, 270-275.] | |
[37] | Terzioglu S, Coskuncelebi K, Gultepe M (2012) Primula × uzungolensis (Primulaceae): a new natural hybrid from NE Anatolia. Turkish Journal of Botany, 36, 9-19. |
[38] | Thompson JD, Gibson T, Higgins DG (2002) Multiple sequence alignment using Clustal W and Clustal X. In: Current Protocols in Bioinformatics (ed. Baxevanis AD). John Wiley & Sons, New York. |
[39] | Tiffin P, Olson MS, Moyle LC (2001) Asymmetrical crossing barriers in angiosperms. Proceedings of the Royal Society B: Biological Sciences, 268, 861-867. |
[40] | van der Velde M, Bijlsma R (2004) Hybridization and asymmetric reproductive isolation between the closely related bryophyte taxa Polytrichum commune and P. uliginosum. Molecular Ecology, 13, 1447-1454. |
[41] | van Droogenbroeck B, Kyndt T, Romeijn-Peeters E, van Thuyne W, Goetghebeur P, Romero-Motochi JP, Gheysen G (2006) Evidence of natural hybridization and introgression between Vasconcellea species (Caricaceae) from southern Ecuador revealed by chloroplast, mitochondrial and nuclear DNA markers. Annals of Botany, 97, 793-805. |
[42] | Wang XR, Szmidt AE, Savolainen O (2001) Genetic composition and diploid hybrid speciation of a high mountain pine, Pinus densata, native to the Tibetan Plateau. Genetics, 159, 337-346. |
[43] | White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications (eds Innis MA, Gelfand DH, Sninsky JJ, White TJ), pp. 315-322. Academic Press, New York. |
[44] | Wu ZK, Zhang CQ (2010) Comparative study of pollination biology of two closely related alpine Primula species, namely Primula beesiana and P. bulleyana (Primulaceae). Journal of Systematics and Evolution, 48, 109-117. |
[45] | Xiang LL, Werth CR, Emery SN, McCauley DE (2000) Population-specific gender-biased hybridization between Dryopteris intermedia and D. carthusiana: evidence from chloroplast DNA. American Journal of Botany, 87, 1175-1180. |
[46] | Yu JJ, Kuroda C, Gong X (2014) Natural hybridization and introgression between Ligularia cymbulifera and L. tongolensis (Asteraceae, Senecioneae) in four different locations. PLoS ONE, 9, e115167. |
[47] | Zha HG, Milne RI, Sun H (2008) Morphological and molecular evidence of natural hybridization between two distantly related Rhododendron species from the Sino-Himalaya. Botanical Journal of the Linnean Society, 156, 119-129. |
[48] | Zha HG, Milne RI, Sun H (2010) Asymmetric hybridization in Rhododendron agastuma: hybrid taxon comprising mainly F1s in Yunnan, China. Annals of Botany, 105, 89-100. |
[49] | Zhang JJ, Montgomery BR, Huang SQ (2016) Evidence for asymmetrical hybridization despite pre- and post-pollination reproductive barriers between two Silene species. Aob Plants, 8, plw032. |
[50] | Zhou RC, Gong X, Boufford D, Wu CI, Shi SH (2008) Testing a hypothesis of unidirectional hybridization in plants: observations on Sonneratia, Bruguiera and Ligularia. BMC Evolutionary Biology, 8, 149. |
[1] | Abbott R, Albach D, Ansell S, Arntzen JW, Baird SJE, Bierne N, Boughman J, Brelsford A, Buerkle CA, Buggs R (2013) Hybridization and speciation. Journal of Evolutionary Biology, 26, 229-246. |
[2] | Anderson E (1948) Hybridization of the habitat. Evolution, 2, 1-9. |
[3] | Arnold ES, Richards AJ (1998) On the occurrence of unilateral incompatibility in Primula section Aleuritia Duby and the origin of Primula scotica Hook. Botanical Journal of the Linnean Society, 128, 359-368. |
[4] | Arnold ML (1997) Natural Hybridization and Evolution. Oxford University Press, New York. |
[5] | Arnold ML, Ballerini ES, Brothers AN (2012) Hybrid fitness, adaptation and evolutionary diversification: lessons learned from Louisiana Irises. Heredity, 108, 159-166. |
[6] | Balao F, Casimiro-Soriguer R, Luis GCJ, Terrab A, Talavera S (2015) Big thistle eats the little thistle: does unidirectional introgressive hybridization endanger the conservation of Onopordum hinojense? New Phytologist, 206, 448-458. |
[7] | Barton NH, Hewitt GM (1985) Analysis of hybrid zones. Annual Review of Ecology and Systematics, 16, 113-148. |
[8] | Broyles SB (2002) Hybrid bridges to gene flow: a case study in milkweeds (Asclepias). Evolution, 56, 1943-1953. |
[9] | Carney SE, Gardner KA, Rieseberg LH (2000) Evolutionary changes over the fifty-year history of a hybrid population of sunflowers (Helianthus). Evolution, 54, 462-474. |
[10] | Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772. |
[11] | Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochemical Bulletin, 19, 11-15. |
[12] | Du GH, Zhang ZQ, Li QJ (2012) Morphological and molecular evidence for natural hybridization in sympatric population of Roscoea humeana and R. cautleoides (Zingiberaceae). Journal of Plant Research, 125, 595-603. |
[13] | Ellstrand NC, Prentice HC, Hancock JF (1999) Gene flow and introgression from domesticated plants into their wild relatives. Annual Review of Ecology and Systematics, 30, 539-563. |
[14] | Field DL, Ayre DJ, Whelan RJ, Young AG (2011) The importance of pre-mating barriers and the local demographic context for contemporary mating patterns in hybrid zones of Eucalyptus aggregata and Eucalyptus rubida. Molecular Ecology, 20, 2367-2379. |
[15] | Guindon S, Gascuel O (2003) A simple, fast and accurate method to estimate large phylogenies by maximum- likelihood. Systematic Biology, 52, 696-704. |
[16] | Hall TA (1999) BioEdita user-friendly biological sequence alignment editor and analysis program for Windows 95/98/ NT. Nucleic Acids Symposium Series, 41, 95-98. |
[17] | Huelsenbeck JP, Ronquist F (2001) MRBAYES Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754-755. |
[18] | Jensen AB, Palmer KA, Boomsma JJ, Pedersen BV (2005) Varying degrees of Apis mellifera ligustica introgression in protected populations of the black honeybee, Apis mellifera mellifera, in Northwest Europe. Molecular Ecology, 14, 93-106. |
[19] | Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH (2005) Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences, USA, 102, 8369-8374. |
[20] | Levin DA, Francisco-Ortega J, Jansen RK (1996) Hybridization and the extinction of rare plant species. Conservation Biology, 10, 10-16. |
[21] | Li XJ, Wang LY, Yang HL, Liu JQ (2007) Confirmation of natural hybrids between Gentiana straminea and G. siphonantha (Gentianaceae) based on molecular evidence. Acta Botanica Yunnanica, 29, 91-97. (in Chinese with English abstract) |
[李小娟, 王留阳, 杨惠玲, 刘建全 (2007) 麻花艽和管花秦艽(龙胆科)之间自然杂交类型的分子验证. 云南植物研究, 29, 91-97.] | |
[22] | Li XW, Li J (1993) A preliminary floristic study on the seed plants from the region of Hengduan Mountain. Acta Botanica Yunnanica, 15, 217-231. (in Chinese with English abstract) |
[李锡文, 李捷 (1993) 横断山脉地区种子植物区系的初步研究. 云南植物研究, 15, 217-231.] | |
[23] | Liao RL, Ma YP, Gong WC, Chen G, Sun WB, Zhou RC, Marczewski T (2015) Natural hybridization and asymmetric introgression at the distribution margin of two Buddleja species with a large overlap. BMC Plant Biology, 15, 146. |
[24] | Ma YP, Xie WJ, Tian XL, Sun WB, Wu ZK, Milne R (2014) Unidirectional hybridization and reproductive barriers between two heterostylous primrose species in north-west Yunnan, China. Annals of Botany, 113, 763-775. |
[25] | Mallet J (2005) Hybridization as an invasion of the genome. Trends in Ecology & Evolution, 20, 229-237. |
[26] | Mao JF, Wang XR (2011) Distinct niche divergence characterizes the homoploid hybrid speciation of Pinus densata on the Tibetan Plateau. The American Naturalist, 177, 424-439. |
[27] | Muranishi S, Tamaki I, Setsuko S, Tomaru N (2013) Asymmetric introgression between Magnolia stellata and M. salicifolia at a site where the two species grow sympatrically. Tree Genetics & Genomes, 9, 1005-1015. |
[28] | Mutke J, Barthlott W (2005) Patterns of vascular plant diversity at continental to global scales. In: Plant Diversity and Complexity Patterns, Local, Regional and Global Dimensions (eds Friis I, Balslev H), pp. 521-537. The Royal Danish Academy of Sciences and Letters, Copenhagen, Biologiske Skrifter. |
[29] | Myers N, Mittermeier RA, Mittermeier CG, da Fonseca GAB, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature, 403, 853-858. |
[30] | Natalis LC, Wesselingh RA (2012) Post-pollination barriers and their role in asymmetric hybridization in Rhinanthus (Orobanchaceae). American Journal of Botany, 99, 1847-1856. |
[51] | Zhu XF, Li Y, Wu GL, Fang ZD, Li QJ, Liu JQ (2009) Molecular and morphological evidence for natural hybridization between Primula secundiflora Franchet and P. poissonii Franchet (Primulaceae). Acta Biologica Cracoviensia Series Botanica, 51, 29-36. |
[31] | Noyes RD (2006) Intraspecific nuclear ribosomal DNA divergence and reticulation in sexual diploid Erigeron strigosus (Asteraceae). American Journal of Botany, 93, 470-479. |
[1] | Liyuan Yang, Ruiwu Wang. Asymmetric interactions in fig-fig wasp mutualism [J]. Biodiv Sci, 2020, 28(11): 1324-1332. |
[2] | Cheng Lu, Yupeng Geng, Ruiwu Wang. Asymmetric interaction and its effects on the meta-population dynamics in co-evolved fig-fig wasps systems [J]. Biodiv Sci, 2012, 20(3): 264-269. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
Copyright © 2022 Biodiversity Science
Editorial Office of Biodiversity Science, 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: 010-62836137, 62836665 E-mail: biodiversity@ibcas.ac.cn