Effect of Cry1Ab/c protein residues from Bt cotton-producing areas in regions with marginal water in three provinces in eastern China

doi:10.17520/biods.2016142

Abstract

Abstract:

To investigate Cry1Ab/c protein residues in marginal water regions of transgenic cotton-producing areas, this study focused on 15 sampling points from three provinces of eastern China using random sampling. We sampled three consecutive years during the flowering and harvesting of cotton, respectively, purified Cry1Ab/c proteins in these samples were detected using the ELISA (enzyme-linked immunosorbent assay) method. Results showed that: (1) The highest concentration of Cry1Ab/c protein in marginal water reached 0.4 ppb at one sampling point. At the other four monitoring points, the residual concentration of Cry1Ab/c protein was less than 0.04 ppb. (2) The closer the cotton field, the higher the positive detection rate, and the positive rate was 13.3% in the cotton field canals. (3) The positive protein detection rate was 12.4% in planting fields that had been planted for more than 7 years. We also found that during the harvest season the positive protein detection rate was easier to detect than during the flowering period. Our findings indicated that the Bt cotton-producing areas should be properly monitored during the harvest season, in order to reduce the potential impacts of Cry1Ab/c protein on the marginal water sources in transgenic cotton producing areas.

Key words: transgenic cotton, enzyme-linked immunosorbent assay (ELISA), Cry1Ab/c protein residues

Hua Liu, Xiaolei Chang, Wei Jiang, Lan Bai, Shufeng Zheng, Jinbin Wang, Wei Wang, Aihu Pan, Rongtan Wang, Xueming Tang. Effect of Cry1Ab/c protein residues from Bt cotton-producing areas in regions with marginal water in three provinces in eastern China[J]. Biodiv Sci, 2016, 24(12): 1373-1380.

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URL: https://www.biodiversity-science.net/EN/10.17520/biods.2016142

https://www.biodiversity-science.net/EN/Y2016/V24/I12/1373

Figures/Tables 6

References 24

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棉产区 Cotton cultivated areas	经纬度 GPS coordinates	不同时间段采样编号 Sample number at different time frames
棉产区 Cotton cultivated areas	经纬度 GPS coordinates	2012.11	2013.7	2013.11	2014.7	2014.11
山东省 Shandong Province
东营三顷三村 Sanqingsan Village of Dongying City	37°52′48″ N, 116°20′11″ E	72(ABC)	80(ABC)	10(ABC)	42(ABC)	96(ABC)
德州宋楼村 Songlou Village of Dezhou City	36°54′37″ N, 115°55′56″ E	58(ABC)	87(ABC)	25(ABC)	43(ABC)	97(ABC)
滨州南赵村 Nanzhao Village of Binzhou City	37°41′7″ N, 118°5′22″ E	75(ABC)	81(ABC)	8(ABC)	46(ABC)	98(ABC)
德州陶店村 Taodian Village of Dezhou City	36°56′15″ N, 115°57′24″ E	73(ABC)	82(ABC)	11(ABC)	44(ABC)	99(ABC)
滨州西黄村 Xihuang Village of Binzhou City	37°52′5″ N, 117°46′11″ E	76(ABC)	83(ABC)	9(ABC)	45(ABC)	100(ABC)
江苏省 Jiangsu Province
江苏省农业科学院 Jiangsu Academy of Agricultural Sciences	32°2′17″ N, 118°51′50″ E	52(ABC)	91(ABC)	-	41(ABC)	95(ABC)
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	33°24′09″ N, 120°34′54″ E	54(ABC)	24(ABC)	2(ABC)	38(ABC)	93(ABC)
泰州荻垛村 Diduo Village of Taizhou City	32°54′19″ N, 120°5′39″ E	68(ABC)	22(ABC)	1(ABC)	39(ABC)	92(ABC)
启东兴益村 Xingyi Village of Qidong City	31°57′5″ N, 121°46′2″ E	56(ABC)	21(ABC)	4(ABC)	40(ABC)	94(ABC)
安徽省 Anhui Province
安庆棉花所 Anqing Cotton Research Institute	30°31′2″ N, 117°5′19″ E	51(ABC)	19(ABC)	28(ABC)	35(ABC)	103(ABC)
宿松复兴村 Fuxing Village of Susong City	29°54′58″ N, 116°30′23″ E	65(ABC)	20(ABC)	30(ABC)	33(ABC)	102(ABC)
望江雷池村 Leichi Village of Wangjiang City	30°9′45″ N, 116°48′43″ E	67(ABC)	14(ABC)	26(ABC)	34(ABC)	101(ABC)
全椒县 Quanjiao County	32°4′18″ N, 118°12′12″ E	49(ABC)	13(ABC)	29(ABC)	77(ABC)	106(ABC)
东至县 Dongzhi County	30°30′55″ N, 117°3′45″ E	48(ABC)	12(ABC)	32(ABC)	36(ABC)	105(ABC)
铜陵市 Tongling City	31°10′24″ N, 117°7′48″ E	50(ABC)	15(ABC)	31(ABC)	37(ABC)	107(ABC)

棉产区 Cotton cultivated areas	经纬度 GPS coordinates	不同时间段采样编号 Sample number at different time frames
棉产区 Cotton cultivated areas	经纬度 GPS coordinates	2012.11	2013.7	2013.11	2014.7	2014.11
山东省 Shandong Province
东营三顷三村 Sanqingsan Village of Dongying City	37°52′48″ N, 116°20′11″ E	72(ABC)	80(ABC)	10(ABC)	42(ABC)	96(ABC)
德州宋楼村 Songlou Village of Dezhou City	36°54′37″ N, 115°55′56″ E	58(ABC)	87(ABC)	25(ABC)	43(ABC)	97(ABC)
滨州南赵村 Nanzhao Village of Binzhou City	37°41′7″ N, 118°5′22″ E	75(ABC)	81(ABC)	8(ABC)	46(ABC)	98(ABC)
德州陶店村 Taodian Village of Dezhou City	36°56′15″ N, 115°57′24″ E	73(ABC)	82(ABC)	11(ABC)	44(ABC)	99(ABC)
滨州西黄村 Xihuang Village of Binzhou City	37°52′5″ N, 117°46′11″ E	76(ABC)	83(ABC)	9(ABC)	45(ABC)	100(ABC)
江苏省 Jiangsu Province
江苏省农业科学院 Jiangsu Academy of Agricultural Sciences	32°2′17″ N, 118°51′50″ E	52(ABC)	91(ABC)	-	41(ABC)	95(ABC)
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	33°24′09″ N, 120°34′54″ E	54(ABC)	24(ABC)	2(ABC)	38(ABC)	93(ABC)
泰州荻垛村 Diduo Village of Taizhou City	32°54′19″ N, 120°5′39″ E	68(ABC)	22(ABC)	1(ABC)	39(ABC)	92(ABC)
启东兴益村 Xingyi Village of Qidong City	31°57′5″ N, 121°46′2″ E	56(ABC)	21(ABC)	4(ABC)	40(ABC)	94(ABC)
安徽省 Anhui Province
安庆棉花所 Anqing Cotton Research Institute	30°31′2″ N, 117°5′19″ E	51(ABC)	19(ABC)	28(ABC)	35(ABC)	103(ABC)
宿松复兴村 Fuxing Village of Susong City	29°54′58″ N, 116°30′23″ E	65(ABC)	20(ABC)	30(ABC)	33(ABC)	102(ABC)
望江雷池村 Leichi Village of Wangjiang City	30°9′45″ N, 116°48′43″ E	67(ABC)	14(ABC)	26(ABC)	34(ABC)	101(ABC)
全椒县 Quanjiao County	32°4′18″ N, 118°12′12″ E	49(ABC)	13(ABC)	29(ABC)	77(ABC)	106(ABC)
东至县 Dongzhi County	30°30′55″ N, 117°3′45″ E	48(ABC)	12(ABC)	32(ABC)	36(ABC)	105(ABC)
铜陵市 Tongling City	31°10′24″ N, 117°7′48″ E	50(ABC)	15(ABC)	31(ABC)	37(ABC)	107(ABC)

	检测结果 Detected results
	2012.11	2013.7	2013.11	2014.7	2014.11
(1)棉田内流动水渠(距离棉田< 50 m) The canal flow in cotton fields (distance from cotton < 50 m)
德州陶店村 Taodian Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
江苏省农业科学院 Jiangsu Academy of Agricultural Sciences	- - -	+ - +		- - -	+ + +
安庆棉花所 Anqing Cotton Research Institute	- - -	- - -	- - -	- - -	- - -
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	- - -	- - -	- - -	- - -	- - -
(2)棉田河岸流动河道(距离棉田< 100 m) The river flowing in the river of cotton field edge (distance from cotton < 100 m)
德州宋楼村 Songlou Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
宿松复兴村 Fuxing Village of Susong City	- - -	- - -	- - -	- - -	+ + +
望江雷池村 Leichi Village of Wangjiang City	- - -	- - -	- - -	- - -	- - -
(3)棉田内封闭水渠 Closed canal in the cotton fields
东营三顷三村 Sanqingsan Village of Dongying City	- - -	- - -	- - -	- - -	+ + +
全椒县 Quanjiao County	- - -	- - -	- + +	- - -	- - -
东至县 Dongzhi County	+ + +	- - -	- - -	- - -	- - -
泰州荻垛村 Diduo Village of Taizhou City	- - -	- - -	- - -	- - -	- - -
(4)棉田附近流动河道(方圆半径< 1,000 m) The river flowing near cotton fields (radius < 1,000 m)
滨州西黄村 Xihuang Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
启东兴益村 Xingyi Village of Qidong City	- - -	- - -	- - -	- - -	- - -
滨州南赵村 Nanzhao Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
铜陵市 Tongling City	- - -	- - -	- - -	- - -	- - -

	检测结果 Detected results
	2012.11	2013.7	2013.11	2014.7	2014.11
(1)棉田内流动水渠(距离棉田< 50 m) The canal flow in cotton fields (distance from cotton < 50 m)
德州陶店村 Taodian Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
江苏省农业科学院 Jiangsu Academy of Agricultural Sciences	- - -	+ - +		- - -	+ + +
安庆棉花所 Anqing Cotton Research Institute	- - -	- - -	- - -	- - -	- - -
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	- - -	- - -	- - -	- - -	- - -
(2)棉田河岸流动河道(距离棉田< 100 m) The river flowing in the river of cotton field edge (distance from cotton < 100 m)
德州宋楼村 Songlou Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
宿松复兴村 Fuxing Village of Susong City	- - -	- - -	- - -	- - -	+ + +
望江雷池村 Leichi Village of Wangjiang City	- - -	- - -	- - -	- - -	- - -
(3)棉田内封闭水渠 Closed canal in the cotton fields
东营三顷三村 Sanqingsan Village of Dongying City	- - -	- - -	- - -	- - -	+ + +
全椒县 Quanjiao County	- - -	- - -	- + +	- - -	- - -
东至县 Dongzhi County	+ + +	- - -	- - -	- - -	- - -
泰州荻垛村 Diduo Village of Taizhou City	- - -	- - -	- - -	- - -	- - -
(4)棉田附近流动河道(方圆半径< 1,000 m) The river flowing near cotton fields (radius < 1,000 m)
滨州西黄村 Xihuang Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
启东兴益村 Xingyi Village of Qidong City	- - -	- - -	- - -	- - -	- - -
滨州南赵村 Nanzhao Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
铜陵市 Tongling City	- - -	- - -	- - -	- - -	- - -

种植年限 Cultivated years	检测结果 Detected results
种植年限 Cultivated years	2012.11	2013.7	2013.11	2014.7	2014.11
种植年限大于7年 Cultivated more than 7 years
德州宋楼村 Songlou Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
东营三顷三村 Sanqingsan Village of Dongying City	- - -	- - -	- - -	- - -	+ + +
全椒县 Quanjiao County	- - -	- - -	- + +	- - -	- - -
东至县 Dongzhi County	+ + +	- - -	- - -	- - -	- - -
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	- - -	- - -	- - -	- - -	- - -
启东兴益村 Xingyi Village of Qidong City	- - -	- - -	- - -	- - -	- - -
江苏农科院 Jiangsu Academy of Agricultural Sciences	- - -	+ - +		- - -	+ + +
种植年限3至7年 Cultivated between 3 to 7 years
滨州南赵村 Nanzhao Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
德州陶店村 Taodian Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
宿松复兴村 Fuxing Village of Susong City	- - -	- - -	- - -	- - -	+ + +
安庆棉花所 Anqing Cotton Research Institute	- - -	- - -	- - -	- - -	- - -
泰州荻垛村 Diduo Village of Taizhou City	- - -	- - -	- - -	- - -	- - -