生物多样性 ›› 2016, Vol. 24 ›› Issue (12): 1373-1380.DOI: 10.17520/biods.2016142

• 研究报告: 植物多样性 • 上一篇    下一篇

华东三省转Bt基因棉花种植对边际水体中Cry1Ab/c蛋白残留的影响

刘华1,2, 常晓蕾3, 蒋玮2,4, 白蓝1,4, 郑曙峰5, 王金斌1,4, 王维5, 潘爱虎1,4, 王荣谈6, 唐雪明1,2,4,,A;*()   

  1. 1 .农业部转基因植物环境安全监督检验测试(上海)中心, 上海 201106
    2 .上海市农业科学院生物技术研究所, 上海 201106
    3 .上海海洋大学, 上海 201306
    4.上海市农业遗传育种重点实验室, 上海 201106
    5 .安徽省农业科学院棉花研究所, 安徽安庆 246003
    6 .上海瑞丰农业科技有限公司, 上海 201106);
  • 收稿日期:2016-05-20 接受日期:2016-08-24 出版日期:2016-12-20 发布日期:2017-01-10
  • 通讯作者: 唐雪明
  • 基金资助:
    上海市农委青年人才项目(沪农青(2016)1-15)、上海市农委重点攻关项目(2015-4-3)和科技部国际合作项目(CS06-L10)

Effect of Cry1Ab/c protein residues from Bt cotton-producing areas in regions with marginal water in three provinces in eastern China

Hua Liu1,2, Xiaolei Chang3, Wei Jiang2,4, Lan Bai1,4, Shufeng Zheng5, Jinbin Wang1,4, Wei Wang5, Aihu Pan1,4, Rongtan Wang6, Xueming Tang1,2,4,*()   

  1. 1 Supervision, Inspection and Test Center for Environmental Safety of Genetically Modified Crops of Ministry of Agriculture, Shanghai 201106
    2 Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106
    3 Shanghai Ocean University, Shanghai 201306
    4 Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai 201106
    5 Cotton Research Institute of Anhui Academy of Agricultural Sciences (CRI, AAAS), Anqing, Anhui 246003
    6 Shanghai Rui-feng Agro-Technology Company Limited, Shanghai 201106;
  • Received:2016-05-20 Accepted:2016-08-24 Online:2016-12-20 Published:2017-01-10
  • Contact: Tang Xueming

摘要:

为调查转基因棉花种植地区边际水体中的Cry1Ab/c蛋白残留情况, 在华东地区的山东、江苏、安徽三省棉田设置采样点, 连续3年在棉花的花铃期和收获季节, 对棉区地块内部及周围边际水体随机采样, 进行去杂及纯化处理后, 利用ELISA (酶联免疫吸附测定)方法检测水样中的Cry1Ab/c蛋白含量。结果表明: (1)在花铃期和收获季前后两周, 分别在5个布控点边际水体中检出Cry1Ab/c蛋白, 其中1个布控点阳性蛋白残留浓度最高达到0.4 ppb, 另外4个布控点检测出的阳性蛋白量均在0.04 ppb以下; (2)距离棉田越近, 蛋白检出阳性率越高, 其中棉田内水渠阳性率为13.3%; (3)连续种植时间超过7年的田地周围水体中蛋白阳性率为12.4%。在所有取样时间点中, 与花铃期相比, 收获季更容易检测到阳性结果。这表明在转基因棉花产区, 应在收获季进行适当的指导和监控, 以预防和降低转基因棉花中Cry1Ab/c蛋白对边际水体的潜在影响。

关键词: 转基因棉花, 酶联免疫吸附测定(ELISA), Cry1Ab/c蛋白含量

Abstract

To investigate Cry1Ab/c protein residues in marginal water regions of transgenic cotton-producing areas, this study focused on 15 sampling points from three provinces of eastern China using random sampling. We sampled three consecutive years during the flowering and harvesting of cotton, respectively, purified Cry1Ab/c proteins in these samples were detected using the ELISA (enzyme-linked immunosorbent assay) method. Results showed that: (1) The highest concentration of Cry1Ab/c protein in marginal water reached 0.4 ppb at one sampling point. At the other four monitoring points, the residual concentration of Cry1Ab/c protein was less than 0.04 ppb. (2) The closer the cotton field, the higher the positive detection rate, and the positive rate was 13.3% in the cotton field canals. (3) The positive protein detection rate was 12.4% in planting fields that had been planted for more than 7 years. We also found that during the harvest season the positive protein detection rate was easier to detect than during the flowering period. Our findings indicated that the Bt cotton-producing areas should be properly monitored during the harvest season, in order to reduce the potential impacts of Cry1Ab/c protein on the marginal water sources in transgenic cotton producing areas.

Key words: transgenic cotton, enzyme-linked immunosorbent assay (ELISA), Cry1Ab/c protein residues