华东三省转Bt基因棉花种植对边际水体中Cry1Ab/c蛋白残留的影响

doi:10.17520/biods.2016142

生物多样性 ›› 2016, Vol. 24 ›› Issue (12): 1373-1380. DOI: 10.17520/biods.2016142

• 研究报告: 植物多样性 • 上一篇下一篇

华东三省转Bt基因棉花种植对边际水体中Cry1Ab/c蛋白残留的影响

刘华^1,², 常晓蕾³, 蒋玮^2,⁴, 白蓝^1,⁴, 郑曙峰⁵, 王金斌^1,⁴, 王维⁵, 潘爱虎^1,⁴, 王荣谈⁶, 唐雪明^1,^2,^4,,A;^*()

1 .农业部转基因植物环境安全监督检验测试(上海)中心, 上海 201106
2 .上海市农业科学院生物技术研究所, 上海 201106
3 .上海海洋大学, 上海 201306
4.上海市农业遗传育种重点实验室, 上海 201106
5 .安徽省农业科学院棉花研究所, 安徽安庆 246003
6 .上海瑞丰农业科技有限公司, 上海 201106);

收稿日期:2016-05-20 接受日期:2016-08-24 出版日期:2016-12-20 发布日期:2017-01-10
通讯作者: 唐雪明
基金资助:
上海市农委青年人才项目(沪农青(2016)1-15)、上海市农委重点攻关项目(2015-4-3)和科技部国际合作项目(CS06-L10)

Effect of Cry1Ab/c protein residues from Bt cotton-producing areas in regions with marginal water in three provinces in eastern China

Hua Liu^1,², Xiaolei Chang³, Wei Jiang^2,⁴, Lan Bai^1,⁴, Shufeng Zheng⁵, Jinbin Wang^1,⁴, Wei Wang⁵, Aihu Pan^1,⁴, Rongtan Wang⁶, Xueming Tang^1,^2,^4,^*()

1 Supervision, Inspection and Test Center for Environmental Safety of Genetically Modified Crops of Ministry of Agriculture, Shanghai 201106
2 Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106
3 Shanghai Ocean University, Shanghai 201306
4 Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai 201106
5 Cotton Research Institute of Anhui Academy of Agricultural Sciences (CRI, AAAS), Anqing, Anhui 246003
6 Shanghai Rui-feng Agro-Technology Company Limited, Shanghai 201106;

Received:2016-05-20 Accepted:2016-08-24 Online:2016-12-20 Published:2017-01-10
Contact: Tang Xueming

摘要/Abstract

摘要：

为调查转基因棉花种植地区边际水体中的Cry1Ab/c蛋白残留情况, 在华东地区的山东、江苏、安徽三省棉田设置采样点, 连续3年在棉花的花铃期和收获季节, 对棉区地块内部及周围边际水体随机采样, 进行去杂及纯化处理后, 利用ELISA (酶联免疫吸附测定)方法检测水样中的Cry1Ab/c蛋白含量。结果表明: (1)在花铃期和收获季前后两周, 分别在5个布控点边际水体中检出Cry1Ab/c蛋白, 其中1个布控点阳性蛋白残留浓度最高达到0.4 ppb, 另外4个布控点检测出的阳性蛋白量均在0.04 ppb以下; (2)距离棉田越近, 蛋白检出阳性率越高, 其中棉田内水渠阳性率为13.3%; (3)连续种植时间超过7年的田地周围水体中蛋白阳性率为12.4%。在所有取样时间点中, 与花铃期相比, 收获季更容易检测到阳性结果。这表明在转基因棉花产区, 应在收获季进行适当的指导和监控, 以预防和降低转基因棉花中Cry1Ab/c蛋白对边际水体的潜在影响。

关键词: 转基因棉花, 酶联免疫吸附测定(ELISA), Cry1Ab/c蛋白含量

Abstract

To investigate Cry1Ab/c protein residues in marginal water regions of transgenic cotton-producing areas, this study focused on 15 sampling points from three provinces of eastern China using random sampling. We sampled three consecutive years during the flowering and harvesting of cotton, respectively, purified Cry1Ab/c proteins in these samples were detected using the ELISA (enzyme-linked immunosorbent assay) method. Results showed that: (1) The highest concentration of Cry1Ab/c protein in marginal water reached 0.4 ppb at one sampling point. At the other four monitoring points, the residual concentration of Cry1Ab/c protein was less than 0.04 ppb. (2) The closer the cotton field, the higher the positive detection rate, and the positive rate was 13.3% in the cotton field canals. (3) The positive protein detection rate was 12.4% in planting fields that had been planted for more than 7 years. We also found that during the harvest season the positive protein detection rate was easier to detect than during the flowering period. Our findings indicated that the Bt cotton-producing areas should be properly monitored during the harvest season, in order to reduce the potential impacts of Cry1Ab/c protein on the marginal water sources in transgenic cotton producing areas.

Key words: transgenic cotton, enzyme-linked immunosorbent assay (ELISA), Cry1Ab/c protein residues

刘华, 常晓蕾, 蒋玮, 白蓝, 郑曙峰, 王金斌, 王维, 潘爱虎, 王荣谈, 唐雪明 (2016) 华东三省转Bt基因棉花种植对边际水体中Cry1Ab/c蛋白残留的影响. 生物多样性, 24, 1373-1380. DOI: 10.17520/biods.2016142.

Hua Liu, Xiaolei Chang, Wei Jiang, Lan Bai, Shufeng Zheng, Jinbin Wang, Wei Wang, Aihu Pan, Rongtan Wang, Xueming Tang (2016) Effect of Cry1Ab/c protein residues from Bt cotton-producing areas in regions with marginal water in three provinces in eastern China. Biodiversity Science, 24, 1373-1380. DOI: 10.17520/biods.2016142.

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链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2016142

https://www.biodiversity-science.net/CN/Y2016/V24/I12/1373

图/表 6

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棉产区 Cotton cultivated areas	经纬度 GPS coordinates	不同时间段采样编号 Sample number at different time frames
棉产区 Cotton cultivated areas	经纬度 GPS coordinates	2012.11	2013.7	2013.11	2014.7	2014.11
山东省 Shandong Province
东营三顷三村 Sanqingsan Village of Dongying City	37°52′48″ N, 116°20′11″ E	72(ABC)	80(ABC)	10(ABC)	42(ABC)	96(ABC)
德州宋楼村 Songlou Village of Dezhou City	36°54′37″ N, 115°55′56″ E	58(ABC)	87(ABC)	25(ABC)	43(ABC)	97(ABC)
滨州南赵村 Nanzhao Village of Binzhou City	37°41′7″ N, 118°5′22″ E	75(ABC)	81(ABC)	8(ABC)	46(ABC)	98(ABC)
德州陶店村 Taodian Village of Dezhou City	36°56′15″ N, 115°57′24″ E	73(ABC)	82(ABC)	11(ABC)	44(ABC)	99(ABC)
滨州西黄村 Xihuang Village of Binzhou City	37°52′5″ N, 117°46′11″ E	76(ABC)	83(ABC)	9(ABC)	45(ABC)	100(ABC)
江苏省 Jiangsu Province
江苏省农业科学院 Jiangsu Academy of Agricultural Sciences	32°2′17″ N, 118°51′50″ E	52(ABC)	91(ABC)	-	41(ABC)	95(ABC)
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	33°24′09″ N, 120°34′54″ E	54(ABC)	24(ABC)	2(ABC)	38(ABC)	93(ABC)
泰州荻垛村 Diduo Village of Taizhou City	32°54′19″ N, 120°5′39″ E	68(ABC)	22(ABC)	1(ABC)	39(ABC)	92(ABC)
启东兴益村 Xingyi Village of Qidong City	31°57′5″ N, 121°46′2″ E	56(ABC)	21(ABC)	4(ABC)	40(ABC)	94(ABC)
安徽省 Anhui Province
安庆棉花所 Anqing Cotton Research Institute	30°31′2″ N, 117°5′19″ E	51(ABC)	19(ABC)	28(ABC)	35(ABC)	103(ABC)
宿松复兴村 Fuxing Village of Susong City	29°54′58″ N, 116°30′23″ E	65(ABC)	20(ABC)	30(ABC)	33(ABC)	102(ABC)
望江雷池村 Leichi Village of Wangjiang City	30°9′45″ N, 116°48′43″ E	67(ABC)	14(ABC)	26(ABC)	34(ABC)	101(ABC)
全椒县 Quanjiao County	32°4′18″ N, 118°12′12″ E	49(ABC)	13(ABC)	29(ABC)	77(ABC)	106(ABC)
东至县 Dongzhi County	30°30′55″ N, 117°3′45″ E	48(ABC)	12(ABC)	32(ABC)	36(ABC)	105(ABC)
铜陵市 Tongling City	31°10′24″ N, 117°7′48″ E	50(ABC)	15(ABC)	31(ABC)	37(ABC)	107(ABC)

棉产区 Cotton cultivated areas	经纬度 GPS coordinates	不同时间段采样编号 Sample number at different time frames
棉产区 Cotton cultivated areas	经纬度 GPS coordinates	2012.11	2013.7	2013.11	2014.7	2014.11
山东省 Shandong Province
东营三顷三村 Sanqingsan Village of Dongying City	37°52′48″ N, 116°20′11″ E	72(ABC)	80(ABC)	10(ABC)	42(ABC)	96(ABC)
德州宋楼村 Songlou Village of Dezhou City	36°54′37″ N, 115°55′56″ E	58(ABC)	87(ABC)	25(ABC)	43(ABC)	97(ABC)
滨州南赵村 Nanzhao Village of Binzhou City	37°41′7″ N, 118°5′22″ E	75(ABC)	81(ABC)	8(ABC)	46(ABC)	98(ABC)
德州陶店村 Taodian Village of Dezhou City	36°56′15″ N, 115°57′24″ E	73(ABC)	82(ABC)	11(ABC)	44(ABC)	99(ABC)
滨州西黄村 Xihuang Village of Binzhou City	37°52′5″ N, 117°46′11″ E	76(ABC)	83(ABC)	9(ABC)	45(ABC)	100(ABC)
江苏省 Jiangsu Province
江苏省农业科学院 Jiangsu Academy of Agricultural Sciences	32°2′17″ N, 118°51′50″ E	52(ABC)	91(ABC)	-	41(ABC)	95(ABC)
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	33°24′09″ N, 120°34′54″ E	54(ABC)	24(ABC)	2(ABC)	38(ABC)	93(ABC)
泰州荻垛村 Diduo Village of Taizhou City	32°54′19″ N, 120°5′39″ E	68(ABC)	22(ABC)	1(ABC)	39(ABC)	92(ABC)
启东兴益村 Xingyi Village of Qidong City	31°57′5″ N, 121°46′2″ E	56(ABC)	21(ABC)	4(ABC)	40(ABC)	94(ABC)
安徽省 Anhui Province
安庆棉花所 Anqing Cotton Research Institute	30°31′2″ N, 117°5′19″ E	51(ABC)	19(ABC)	28(ABC)	35(ABC)	103(ABC)
宿松复兴村 Fuxing Village of Susong City	29°54′58″ N, 116°30′23″ E	65(ABC)	20(ABC)	30(ABC)	33(ABC)	102(ABC)
望江雷池村 Leichi Village of Wangjiang City	30°9′45″ N, 116°48′43″ E	67(ABC)	14(ABC)	26(ABC)	34(ABC)	101(ABC)
全椒县 Quanjiao County	32°4′18″ N, 118°12′12″ E	49(ABC)	13(ABC)	29(ABC)	77(ABC)	106(ABC)
东至县 Dongzhi County	30°30′55″ N, 117°3′45″ E	48(ABC)	12(ABC)	32(ABC)	36(ABC)	105(ABC)
铜陵市 Tongling City	31°10′24″ N, 117°7′48″ E	50(ABC)	15(ABC)	31(ABC)	37(ABC)	107(ABC)

	检测结果 Detected results
	2012.11	2013.7	2013.11	2014.7	2014.11
(1)棉田内流动水渠(距离棉田< 50 m) The canal flow in cotton fields (distance from cotton < 50 m)
德州陶店村 Taodian Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
江苏省农业科学院 Jiangsu Academy of Agricultural Sciences	- - -	+ - +		- - -	+ + +
安庆棉花所 Anqing Cotton Research Institute	- - -	- - -	- - -	- - -	- - -
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	- - -	- - -	- - -	- - -	- - -
(2)棉田河岸流动河道(距离棉田< 100 m) The river flowing in the river of cotton field edge (distance from cotton < 100 m)
德州宋楼村 Songlou Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
宿松复兴村 Fuxing Village of Susong City	- - -	- - -	- - -	- - -	+ + +
望江雷池村 Leichi Village of Wangjiang City	- - -	- - -	- - -	- - -	- - -
(3)棉田内封闭水渠 Closed canal in the cotton fields
东营三顷三村 Sanqingsan Village of Dongying City	- - -	- - -	- - -	- - -	+ + +
全椒县 Quanjiao County	- - -	- - -	- + +	- - -	- - -
东至县 Dongzhi County	+ + +	- - -	- - -	- - -	- - -
泰州荻垛村 Diduo Village of Taizhou City	- - -	- - -	- - -	- - -	- - -
(4)棉田附近流动河道(方圆半径< 1,000 m) The river flowing near cotton fields (radius < 1,000 m)
滨州西黄村 Xihuang Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
启东兴益村 Xingyi Village of Qidong City	- - -	- - -	- - -	- - -	- - -
滨州南赵村 Nanzhao Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
铜陵市 Tongling City	- - -	- - -	- - -	- - -	- - -

	检测结果 Detected results
	2012.11	2013.7	2013.11	2014.7	2014.11
(1)棉田内流动水渠(距离棉田< 50 m) The canal flow in cotton fields (distance from cotton < 50 m)
德州陶店村 Taodian Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
江苏省农业科学院 Jiangsu Academy of Agricultural Sciences	- - -	+ - +		- - -	+ + +
安庆棉花所 Anqing Cotton Research Institute	- - -	- - -	- - -	- - -	- - -
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	- - -	- - -	- - -	- - -	- - -
(2)棉田河岸流动河道(距离棉田< 100 m) The river flowing in the river of cotton field edge (distance from cotton < 100 m)
德州宋楼村 Songlou Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
宿松复兴村 Fuxing Village of Susong City	- - -	- - -	- - -	- - -	+ + +
望江雷池村 Leichi Village of Wangjiang City	- - -	- - -	- - -	- - -	- - -
(3)棉田内封闭水渠 Closed canal in the cotton fields
东营三顷三村 Sanqingsan Village of Dongying City	- - -	- - -	- - -	- - -	+ + +
全椒县 Quanjiao County	- - -	- - -	- + +	- - -	- - -
东至县 Dongzhi County	+ + +	- - -	- - -	- - -	- - -
泰州荻垛村 Diduo Village of Taizhou City	- - -	- - -	- - -	- - -	- - -
(4)棉田附近流动河道(方圆半径< 1,000 m) The river flowing near cotton fields (radius < 1,000 m)
滨州西黄村 Xihuang Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
启东兴益村 Xingyi Village of Qidong City	- - -	- - -	- - -	- - -	- - -
滨州南赵村 Nanzhao Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
铜陵市 Tongling City	- - -	- - -	- - -	- - -	- - -

种植年限 Cultivated years	检测结果 Detected results
种植年限 Cultivated years	2012.11	2013.7	2013.11	2014.7	2014.11
种植年限大于7年 Cultivated more than 7 years
德州宋楼村 Songlou Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
东营三顷三村 Sanqingsan Village of Dongying City	- - -	- - -	- - -	- - -	+ + +
全椒县 Quanjiao County	- - -	- - -	- + +	- - -	- - -
东至县 Dongzhi County	+ + +	- - -	- - -	- - -	- - -
大丰稻麦原种场 Rice and Wheat Seeds stock station of Dafeng City	- - -	- - -	- - -	- - -	- - -
启东兴益村 Xingyi Village of Qidong City	- - -	- - -	- - -	- - -	- - -
江苏农科院 Jiangsu Academy of Agricultural Sciences	- - -	+ - +		- - -	+ + +
种植年限3至7年 Cultivated between 3 to 7 years
滨州南赵村 Nanzhao Village of Binzhou City	- - -	- - -	- - -	- - -	- - -
德州陶店村 Taodian Village of Dezhou City	- - -	- - -	- - -	- - -	- - -
宿松复兴村 Fuxing Village of Susong City	- - -	- - -	- - -	- - -	+ + +
安庆棉花所 Anqing Cotton Research Institute	- - -	- - -	- - -	- - -	- - -
泰州荻垛村 Diduo Village of Taizhou City	- - -	- - -	- - -	- - -	- - -

华东三省转Bt基因棉花种植对边际水体中Cry1Ab/c蛋白残留的影响

Effect of Cry1Ab/c protein residues from Bt cotton-producing areas in regions with marginal water in three provinces in eastern China

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