生物多样性 ›› 2015, Vol. 23 ›› Issue (6): 759-766.  DOI: 10.17520/biods.2015109

• 研究报告: 植物多样性 • 上一篇    下一篇

青藏高原东北部及其邻近地区无苞香蒲的谱系地理学

丁振杰, 于丹, 徐新伟*()   

  1. 武汉大学生命科学学院, 武汉 430070
  • 收稿日期:2015-05-03 接受日期:2015-11-09 出版日期:2015-11-20 发布日期:2015-12-02
  • 通讯作者: 徐新伟
  • 基金资助:
    国家自然科学基金: 中国水生植物广布种的比较分子谱系地理学研究(31270265)

Phylogeography of Typha laxmannii in the northeast Qinghai-Tibet Plateau and adjacent areas

Zhenjie Ding, Dan Yu, Xinwei Xu*()   

  1. College of Life Sciences, Wuhan University, Wuhan 430070
  • Received:2015-05-03 Accepted:2015-11-09 Online:2015-11-20 Published:2015-12-02
  • Contact: Xu Xinwei

摘要:

第四纪冰期气候的反复变化对青藏高原及邻近地区植物的种群地理分布及种群遗传结构产生了巨大的影响。本研究对青藏高原东北部及其邻近地区无苞香蒲(Typha laxmannii)的15个种群148个个体的叶绿体rpl32-trnL间隔区和核基因(植物螯合肽合成酶, PS)进行测序, 共发现2个叶绿体单倍型和8个核基因单倍型。所有的单倍型被共享, 高原种群没有特有的单倍型。邻近地区种群的叶绿体遗传多样性和核基因遗传多样性分别是高原种群的4倍和2倍。高原种群的遗传分化水平明显高于邻近地区种群, 其中高原种群的遗传分化主要存在于东部种群与西部种群之间。研究结果表明, 冰期后从多个避难所回迁至高原台面和由此产生的奠基者效应造成了无苞香蒲在青藏高原东北及邻近地区目前的遗传多样性和基因谱系地理分布格局。

关键词: Typha laxmannii, 叶绿体rpl32-trnL基因间隔区, 植物螯合肽合成酶基因, 谱系地理

Abstract

Quaternary climatic oscillation had a significant role in the patterns of geographic distribution and genetic structure of plants occurring in Qinghai-Tibet Plateau (QTP) and adjacent areas. In this study, we examined the phylogeographical structure of Typha laxmannii in the northeast QTP and adjacent areas based on 15 populations and 148 individuals using sequences of chloroplast rpl32-trnL and nuclear phytochelatin synthase gene. Two chloroplast haplotypes and eight nuclear haplotypes were found. All haplotypes were shared and no private haplotype was fixed in plateau populations. The genetic diversity of the populations in adjacent areas based on chloroplast DNA and nuclear DNA was four and two times greater than that of plateau populations, respectively. Genetic differentiation among plateau populations, which mainly existed between the eastern and western populations, was higher than that among adjacent areas. Our results indicate that the present patterns of genetic diversity and genetic structure of T. laxmannii in the northeast QTP and its adjacent areas were caused by postglacial recolonization from at least two refugia to plateau platform and a resulting founder effect.

Key words: graceful cattail, chloroplast rpl32-trnL intergenic spacer, phytochelatin synthase, phylogeography