生物多样性 ›› 2009, Vol. 17 ›› Issue (3): 288-295.DOI: 10.3724/SP.J.1003.2009.08251

• 论文 • 上一篇    下一篇

新疆罗布泊地区可培养嗜盐细菌多样性

罗明1,*(), 韩剑1, 蒋平安2, 武红旗2   

  1. 1 新疆农业大学农学院, 乌鲁木齐 830052
    2 新疆农业大学草业与环境科学学院, 乌鲁木齐 830052
  • 收稿日期:2008-09-27 接受日期:2009-05-15 出版日期:2009-05-20 发布日期:2009-05-20
  • 通讯作者: 罗明
  • 作者简介:*E-mail: luomingxjau@yahoo.com.cn
  • 基金资助:
    国家自然科学基金(40671080);新疆特殊环境微生物重点实验室开放项目(XJYS0203-2006-03)

Diversity of culturable halophilic bacteria isolated from Lop Nur region in Xinjiang

Ming Luo1,*(), Jian Han1, Pingan Jiang2, Hongqi Wu2   

  1. 1 Agronomy Faculty, Xinjiang Agricultural University, Urumqi 830052
    2 College of Grassland and Environmental Science, Xinjiang Agricultural University, Urumqi 830052
  • Received:2008-09-27 Accepted:2009-05-15 Online:2009-05-20 Published:2009-05-20
  • Contact: Ming Luo

摘要:

采用纯培养方法从新疆罗布泊盐湖中分离得到168株嗜盐细菌, 采用核糖体DNA扩增片段限制性酶切分析(ARDRA)研究了罗布泊嗜盐细菌的群落结构和多样性。通过限制性内切酶HinfI对108个菌株的16S rDNA进行酶切分型, 根据ARDRA的酶切图谱, 将其划分为12个操作分类单元。16S rDNA序列测定和系统发育分析结果显示, 分离菌株分布于喜盐芽孢杆菌属(Halobacillus)、芽孢杆菌属(Bacillus)、短杆菌属(Brevibacterium)嗜盐单胞菌属(Halomonas)、色盐杆菌属(Chromohalobacter)、盐水球菌属(Salinicoccus)、库克菌属(Kocuria)、葡萄球菌属(Staphylococcus)、微球菌属(Micrococcus)等9个属及1个可能的新分类单元。其中Halomonas为优势菌群, Chromohalobacter、Halobacillus为次优势菌群。采用ERIC-PCR分析优势菌群Halomonas各菌株的基因组特征, 显示出有21种指纹图谱。研究结果揭示, 罗布泊嗜盐细菌不仅具有丰富的多样性, 还蕴藏着具有地域特点的新菌种资源。

关键词: ARDRA, ERIC-PCR, 嗜盐细菌多样性, 极端环境微生物, 菌种资源

Abstract

We investigated the community structure and diversity of the culturable halophilic bacteria in Lop Nur, Xinjiang using amplified ribosomal DNA restriction analysis (ARDRA). Restriction enzyme HinfI was applied in ARDRA of 108 halophilic bacterial strains, and 12 operational taxonomic units (OTUs) were assigned on the basis of the ARDRA patterns. Each OTU was randomly chosen for 16S rRNA gene sequencing. The phylogenetic analysis on the basis of 16S rRNA gene homology showed that all isolates affiliated to nine genera, consisting of Halobacillus, Bacillus, Brevibacterium, Halomonas, Chromohalobacter, Salinicoccus, Kocuria, Staphylococcus, Micrococcus, and a possible novel taxon. Halomonas strains dominanted among the isolates, and followed by Chromohalobacter and Halobacillus members. The strains of Halomonas were performed ERIC-PCR (enterobacterial repetitive intergenic consensus-PCR) analysis, and 21 ERIC-PCR fingerprinting types were determined. The results suggest that the halophilic bacteria are abundant, and some unknown taxa can exist in Lop Nur.

Key words: ARDRA, ERIC-PCR, diversity, extemophiles, culture collection