生物多样性 ›› 2018, Vol. 26 ›› Issue (7): 727-737.DOI: 10.17520/biods.2018026

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腾格里沙漠东南缘藓结皮微生物组基因多样性及功能

李靖宇1,2,*(), 刘建利1,2, 张琇1,2, 王猛1, 杨洲1, 靳新影1, 苟琪1, 张翼飞3   

  1. 1 北方民族大学生物科学与工程学院, 银川 750021
    2 宁夏特殊生境微生物资源开发与利用重点实验室, 银川 750021
    3 内蒙古自治区环境科学研究院, 呼和浩特 010010
  • 收稿日期:2018-01-29 接受日期:2018-03-16 出版日期:2018-07-20 发布日期:2018-09-11
  • 通讯作者: 李靖宇
  • 基金资助:
    国家自然科学基金(41661053)、2018年度北方民族大学校级一般科研项目(2018XYZSK01)和宁夏特色微生物资源开发与利用创新团队科研项目(2018TSW04)

Gene diversity and its function in the soil microbiome for moss crusts found southeast of the Tengger Desert

Li Jingyu1,2,*(), Liu Jianli1,2, Zhang Xiu1,2, Wang Meng1, Yang Zhou1, Jin Xinying1, Gou Qi1, Zhang Yifei3   

  1. 1 College of Biological Science & Engineering, North Minzu University, Yinchuan 750021
    2 Ningxia Key Laboratory of Microbial Resources Development and Applications in Special Environment, Yinchuan 750021
    3 Inner Mongolia Academy of Environmental Science, Hohhot 010010
  • Received:2018-01-29 Accepted:2018-03-16 Online:2018-07-20 Published:2018-09-11
  • Contact: Li Jingyu

摘要:

本研究对腾格里沙漠东南缘沙坡头地区藓结皮土壤样品进行了宏基因组测序, 以揭示该地区藓结皮土壤中参与固碳、固氮等生态功能的微生物基因及其代谢通路。结果表明, 藓结皮土壤中细菌域微生物最多, 其次为古生菌域和真核生物域。在细菌域中, 放线菌门(Actinobacteria)数量最多, 其次是变形菌门(Proteobacteria)和蓝细菌门(Cyanobacteria)。基于eggNOG和KEGG数据库对构建的非冗余基因集进行功能预测, 藓结皮土壤中微生物基因多样性和代谢路径多样性高。藓结皮土壤中与固氮相关的代谢通路丰度低可能是藓结皮固氮量微弱的根本原因, 造成利用大气中氮气合成氨的生态功能减弱。而藓结皮已经形成的氮库主要通过硝酸盐还原途径将硝酸盐还原成铵盐, 可能用于藓结皮微生物组自身的氨基酸合成, 也可能为藓类植物的生长提供有效氮源。

关键词: 微生物群落, 生物土壤结皮, 沙坡头沙漠, HiSeq测序

Abstract:

Metagenomics sequencing was used to reveal the diversity of genes and their metabolic pathways involved in carbon sequestration and nitrogen fixation in moss crusts in Shapotou region, located southeast of the Tengger Desert. Results showed that the microbial community related to the bacteria domain in the moss crust was the largest, followed by archaea and eukaryota. In the bacterial domain, the most abundant phyla was the Actinobacteria, followed by the Proteobacteria and Cyanobacteria. Based on the functional classification of eggNOG and KEGG databases for the functional prediction of non-redundant gene catalog, the diversity of gene and metabolic pathways in the moss crust soil was high. Low nitrogen fixation was found in the moss crust as there are less metabolic pathways related to nitrogen-fixation, weakening the ecological function of ammonia synthesis from atmosphere nitrogen. The nitrate from the nitrogen pool formed by moss crust were mainly reduced to ammonium salt by nitrate reduction pathway, which may be used for the synthesis of amino acids for microbiome in the moss crust, and may also provide an effective nitrogen source for the growth of mosses.

Key words: microbial communities, biological soil crust, Shapotou Desert, HiSeq sequencing