Biodiversity Science ›› 2012, Vol. 20 ›› Issue (2): 224-230.doi: 10.3724/SP.J.1003.2012.07214

• Original Papers • Previous Article     Next Article

Genetic diversity of Armillaria gallica isolates from China and Europe revealed with ISSR analysis

Lifu Sun1*, Kequan Pei2, Yanhua Zhang1, Jun Zhao3, Guoting Yang4, Guofu Qin3, Yushuang Song3, Ruiqing Song4   

  1. 1College of Life Sciences, Shaoxing University, Shaoxing, Zhejiang 312000

    2State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093

    3General Station of Forest Pest Management, State Forestry Administration, Shenyang 110034

    4School of Forestry, Northeast Forestry University, Harbin 150040
  • Received:2011-11-24 Revised:2012-02-10 Online:2012-04-09
  • Lifu Sun

Armillaria gallica is a cosmopolitan fungal species found in the northern hemisphere, and genetic diversity and similarity between different continental isolates of the species can reveal important large-scale biogeographical patterns. A total of 79 isolates of A. gallica were collected from Europe and China. We estimated the genetic diversity of these isolates using ISSR (inter-simple sequence repeat) markers. Six ISSR primers were employed to identify a total of 210 scorable fragments, of which 202 (96.2%) were polymorphic loci (frequecy<0.95). The average number of polymorphic loci detected with each primer was 33.6. The results indicated that ISSR markers can reflect abundant polymorphism in Armillaria. According to UPGMA clustering analysis, 49 out of 53 Chinese A. gallica isolates clustered into a China Group at the 0.773 similarity level. The genetic divergence of European isolates was significantly higher than predicted; these isolates were clustered into Europe Group A and Group B at the 0.775 and 0.763 similarity level, respectively. The similarity coefficient between the two European groups was only 0.738, but it was 0.770 between China Group and Europe Group A. Some isolates from China and Europe had experienced obvious genetic divergence, and several isolates had lower intraspecific similarity coefficients than interspecific similarity coefficient found in other Armillaria biological species. Our results indicated that considerable genetic differentiation existed between European and Chinese isolates due to geographic isolation, and that these isolates were in the process of allopatric speciation. The differentiation among isolates of A. gallica from Europe was more evident, and Europe is possibly the place of origin for isolates of the species.

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[1] Li Shuzhong. Brief iutroduction on the success of captvie breeding of rare birds in the Beijing Zoo[J]. Biodiv Sci, 1994, 02(3): 181 -183 .
[2] WU Fu-Zhong, YANG Wan-Qin, ZHANG Jian, ZHOU Li-Qiang. Effects of cadmium stress on growth and nutrient accumulation, distribution and utilization in Osmanthus fragrans var. thunbergii[J]. Chin J Plan Ecolo, 2010, 34(10): 1220 -1226 .
[3] Wang Pei-fang, Xia Yu-mei. A Preliminary Study of Spore-Pollen Society and it’s Development Process to T302 Pole Section,in Liuhe,Jilin[J]. Chin J Plan Ecolo, 1990, 14(3): 287 -292 .
[4] YANG Ya, WANG Yu-Hua, DU Ning. [J]. Plant Diversity, 2014, 36(02): 267 -270 .
[5] WANG Hong-Xing, CHEN Xin, TANG Jian-Jun, SHIMIZU Katsuyoshi. Influence of released transgenic pest and disease resistant crops on plant associated microorganisms in soil[J]. Biodiv Sci, 2002, 10(2): 232 -237 .
[6] Man Chen, YishengTu, Linan Ye, Biyun Yang. Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata[J]. Chin Bull Bot, 2017, 52(2): 218 -224 .
[7] ZHANG Jia-Ning-, LIU Kun-, WANG Jie-Gong. The Relationship between Seed Germinability and Species Abundance in Communities on Eastern QinghaiTibet Plateau[J]. Plant Diversity, 2013, 35(3): 317 -326 .
[8] WANG Xi-Qun, MA Lu-Yi, GUO Bao-Xiang, GE Ji-Wen, FAN Shen-Hou. The conservation of Metasequoia glyptostroboides and its current problems in China[J]. Biodiv Sci, 2004, 12(3): 377 -385 .
[9] HE Wei-Ming and ZHONG Zhang-Cheng. Effects of Soil Fertility on Gynostemma pentaphyllum Makino Population Behavior[J]. Chin Bull Bot, 1999, 16(04): 425 -428 .
[10] Pengtao Wang, Jing Zhao, Huanhuan Yu. Reactive Oxygen Species Signaling in Stomata[J]. Chin Bull Bot, 2014, 49(4): 490 -503 .