Biodiversity Science ›› 2011, Vol. 19 ›› Issue (4): 485-493.doi: 10.3724/SP.J.1003.2011.09232

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Genetic diversity in rhizosphere soil microbes detected with SRAP markers

Chunnan Li1, 2, Hairui Cui1*, Weibo Wang1   

  1. 1Institute of Nuclear-Agricultural Sciences/Key Laboratory of Nuclear Agricultural Sciences, Ministry of Agriculture, Zhejiang University, Hangzhou 310029

    2Institute of Horticulture, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024
  • Received:2010-09-20 Revised:2011-01-30 Online:2011-07-29
  • Hairui Cui E-mail:hrcui@zju.edu.cn

We have attempted to use the SRAP (sequence-related amplified polymorphism) markers, a new molecular technology, to study genetic diversity in soil microbes. We sampled rhizosphere soil microbes from 20 plant species and employed 22 SRAP primer combinations. A total of 237 scorable fragments were identi-fied, of which 221 (93.2%) were polymorphic loci. The average percentage of polymorphic loci (PPL), po-lymorphism information content (PIC), allele haplotype (Ah), and expected heterozygosity (He) for each primer combination were 93.78%, 0.94, 18.05 and 0.92, respectively. Our results revealed rich genetic diver-sity in rhizosphere soil microbes and the high ability of SRAP to resolve samples based on their genetic basis. Differences in genetic distance for rice rhizosphere microbes between two locations, and that among four different developmental stages were both significant at 0.01 level, but the difference was not significant be-tween two varieties. Shannon diversity indices indicated that the genetic diversity of rhizosphere soil mi-crobes was lowest in rice and highest in lettuce. The rhizosphere soil microbes from 20 plant species could be clustered into three groups at the 0.454 (GD) level based on UPGMA, in which the first group was from rice, while the second group was from celery planted in plastic green house, and the third group was from 18 other plant species cultivated in dry lands. Our results suggest that SRAP is an efficient method for analyzing the genetic diversity in rhizosphere soil microbes.

Key words: scleractinian coral, species diversity, coral associated species

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