Biodiversity Science ›› 2017, Vol. 25 ›› Issue (12): 1267-1275.doi: 10.17520/biods.2017284

• Special Feature: Biological Invasion • Previous Article     Next Article

Physiological response of the invasive weed Mikania micrantha and the native species Pueraria lobata var. thomsonii to water stress

Jiayi Wang1, Hanxia Yu1, Yufang Lai1, Fanghao Wan2, Wanqiang Qian2, Changlian Peng1, Weihua Li1, *()   

  1. 1 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education; School of Life Sciences, South China Normal University, Guangzhou 510631
    2 Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518120
  • Received:2017-10-23 Accepted:2017-12-18 Online:2017-12-10
  • Li Weihua E-mail:whli@scnu.edu.cn

Mikania micrantha is the most dangerous invasive weed in South China, due to its harmful effects on natural secondary forest habitats and other habitats. In order to illustrate the possibility of using the native legume Pueraria lobata var. thomsonii to replace M. micrantha, three water gradients (soil water content < 10%, 60-70% and 120-130%) that simulate drought, normal soil water content (as control) and waterlogged conditions, respectively, were established to study the physiological response of these two plant species to drought and waterlogging. Results showed that under drought stress, the total biomass of M. micrantha decreased significantly by 72% as compared to controls. Under waterlogging stress, the total biomass of P. lobata var. thomsonii increased by 16% compared to the control treatment, while M. micrantha decreased by 15%. Under drought conditions, the root-shoot ratio and chlorophyll content of native species P. lobata var. thomsonii were significantly greater than those found in the control (P < 0.05); while there were no significant differences in the root-shoot ratio of M. micrantha compared to the control (P > 0.05), but the chlorophyll content was significantly higher than that found in the control but lower than the normal level after rewatering. This may be the result of a significant increase in the proline and soluble sugar in P. lobata var. thomsonii. Following waterlogging stress, the malondialdehyde content of P. lobata var. thomsonii was approximately 2.1 times higher than the control, while that of M. micrantha was 3 times the control. After soil water content returned to normal levels, the malondialdehyde content of M. micrantha and P. lobata var. thomsonii maintained high levels, which were 1.72 times and 1.45 times the control group, respectively, indicating that the membrane lipid peroxidation level of P. lobata var. thomsonii was lower than that of M. micrantha as affected by waterlogging. Thus, it was concluded that P. lobata var. thomsonii exhibited better drought and waterlogging tolerance than M. micrantha. The resistance index also confirmed this conclusion. The results provide theoretical support for the control of M. micrantha through replacement by applying the native legume P. lobata var. thomsonii to natural habitats like forest edges or windows in areas of South China.

Key words: Mikania micrantha, Pueraria lobata var. thomsonii, drought, waterlogging, stress resistance

Fig. 1

Changes of root length and plant height of Pueraria lobata var. thomsonii (P) and Mikania micrantha (M) after water stress and restoration to normal water content(mean ± SD, n = 5). Treatments (T), control, drought and waterlogging; Recovery (R), recovery of water content to 60-70%. Dunnett-test, * indicated significant difference between treatment and control group in the same plant (P < 0.05), ** indicated highly significant difference between treatment and control group (P < 0.01)."

Fig. 2

Changes of total biomass and root-shoot ratio of Pueraria lobata var. thomsonii (P) and Mikania micrantha (M) after water stress and restoration to normal water content(mean ± SD, n = 5). The abbreviations and Dunnett-test were same as those in Fig. 1."

Fig. 3

Contents of malondialdehyde (MDA) in Pueraria lobata var. thomsonii (P) and Mikania micrantha (M) after water stress and restoration to normal water content (mean ± SD, n = 5). The abbreviations and Dunnett-test were same as those in Fig. 1."

Fig. 4

Comparison of proline and soluble sugar content between Pueraria lobata var. thomsonii (P) and Mikania micrantha (M) after water stress and restoring to normal water content(mean ± SD, n = 5). The abbreviations and Dunnett-test were same as those in Fig. 1."

Table 1

Changes of chlorophyll content of Pueraria lobata var. thomsonii (P) and Mikania micrantha (M) under water stress (mg/g) (mean ± SD, n = 5)"

土壤含水量
Water content (%)
粉葛处理
P treatments
粉葛恢复
P recovery
薇甘菊处理
M treatments
薇甘菊恢复
M recovery
对照 Control (60-70) 1.76 ± 0.10 1.44 ± 0.08 0.94 ± 0.07 0.40 ± 0.05
干旱 Drought (0-10) 3.35 ± 0.29** 1.43 ± 0.12 1.91 ± 0.07** 0.25 ± 0.03**
涝害 Waterlogging (120-130) 1.59 ± 0.35 1.50 ± 0.09* 1.26 ± 0.20** 0.26 ± 0.02**

Table 2

Comparison of resistance index in Pueraria lobata var. thomsonii (P) and Mikania micrantha (M) under water stress (mean ± SD, n = 3)"

干旱 Drought 涝害 Waterlogging
粉葛 P 薇甘菊 M Dunnett检验 Dunnett-test 粉葛 P 薇甘菊 M Dunnett检验 Dunnett-test
根长 Root length 0.67 ± 0.15 0.73 ± 0.05 ns 0.75 ± 0.08 0.58 ± 0.07 ns
株高 Plant height 0.83 ± 0.04 0.84 ± 0.18 ns 0.88 ± 0.09 0.88 ± 0.04 ns
生物量 Biomass 0.76 ± 0.09 0.57 ± 0.05 * 0.72 ± 0.38 0.76 ± 0.17 ns
丙二醛 Malondialdehyde 0.08 ± 0.001 0.02 ± 0.001 ** -0.02 ± 0.003 -0.33 ± 0.06 **
脯氨酸 Proline 0.58 ± 0.23 -0.69 ± 0.007 ** 0.44 ± 0.03 0.89 ± 0.03 **
可溶性糖 Soluble sugar -0.07 ± 0.07 -0.45 ± 0.13 * 0.32 ± 0.11 0.001 ± 0.04 **
叶绿素 Chlorophyll 0.05 ± 0.01 -0.01 ± 0.02 ** 0.68 ± 0.1 0.54 ± 0.15 ns

Table 3

Comparison of resilience index in Pueraria lobata var. thomsonii (P) and Mikania micrantha (M) under water stress (mean ± SD, n = 3)"

干旱 Drought 涝害 Waterlogging
粉葛 P 薇甘菊 M Dunnett检验 Dunnett-test 粉葛 P 薇甘菊 M Dunnett检验 Dunnett-test
根长 Root length -0.39 ± 0.15 0.31 ± 0.13 ** 0.25 ± 0.03 0.77 ± 0.19 **
株高 Plant height -0.65 ± 0.03 -0.73 ± 0.27 ns -0.64 ± 0.26 -0.52 ± 0.26 ns
生物量 Biomass -0.88 ± 0.04 -0.76 ± 0.02 ** -0.84 ± 0.22 -0.84 ± 0.11 ns
丙二醛 Malondialdehyde 0.66 ± 0.26 0.76 ± 0.11 ns 0.14 ± 0.06 0.34 ± 0.17 ns
脯氨酸 Proline 0.49 ± 0.3 0.85 ± 0.06 ns 0.54 ± 0.06 -0.62 ± 0.27 **
可溶性糖 Soluble sugar -0.17 ± 0.1 0.39 ± 0.06 ** -0.08 ± 0.02 -0.26 ± 0.02 **
叶绿素 Chlorophyll 0.96 ± 0.04 0.75 ± 0.03 ** 0.50 ± 0.09 0.32 ± 0.01 *
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