生物多样性 ›› 2010, Vol. 18 ›› Issue (1): 37-43.doi: 10.3724/SP.J.1003.2010.037

• 论文 • 上一篇    下一篇

甘蔗品种资源的表型遗传多样性

刘新龙1, 2, 蔡青1, 3, 吴才文1, 2, 马丽1, 2, 应雄美1, 2, 陆鑫1, 2, 范源洪1, 2   

  1. 1 (云南省甘蔗遗传改良重点实验室, 云南开远 661600)
    2 (云南省农业科学院甘蔗研究所, 云南开远 661600)
    3 (云南省农业科学院生物技术与种质资源研究所, 昆明 650223)
  • 收稿日期:2009-04-09 出版日期:2010-01-20
  • 通讯作者: 范源洪

Phenotypic diversity of sugarcane variety germplasm

Xinlong Liu1, 2, Qing Cai1, 3, Caiwen Wu1, 2, Li Ma1, 2, Xiongmei Ying1, 2, Xin Lu1, 2, Yuanhong Fan1, 2   

  1. 1 Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, Yunnan 661600
    2 Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan, Yunnan 661600
    3 Biotechnology & Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223
  • Received:2009-04-09 Online:2010-01-20
  • Contact: Yuanhong Fan

为了提高甘蔗品种资源的利用效率, 为甘蔗遗传育种亲本的选择、杂交组合配制及核心种质构建提供理论指导, 我们利用17个质量性状和5个数量性状分析了来自13个国家共20个地区的1,160份甘蔗品种资源的遗传变异、遗传结构和遗传距离等。结果表明: 5个数量性状在不同来源地品种群体之间的变异系数值(CV)变化较大, 说明不同来源地品种群体的数量遗传变异有较大差异, 其中来自海南的品种其群体遗传变异最丰富。质量性状的Shannon-Wiener多样性指数表明, 来自美国的品种群体遗传多样性最高, 其次为中国台湾, 第三为澳大利亚, 说明上述3个地区的甘蔗种质创新比较活跃, 在遗传育种中使用了更多表型性状多样化的亲本。不同来源地品种其群体的遗传分化系数(Gst)和基因流(Nm)显示甘蔗品种群体表型性状的遗传变异主要来自来源地内部, 且不同来源地品种群体之间存在较大的基因交流。遗传距离和UPGMA聚类分析结果表明, 各来源地品种群体之间遗传距离在0.0261–0.2945之间, 其中以福建和广东的品种最为相似, 其次为古巴和美国、广西和云南、澳大利亚和菲律宾、江西和四川、巴西和法国, 说明上述地区在杂交亲本的选择上比较相近。鉴于此, 在遗传育种中应加大利用具有丰富遗传多样性的品种材料并尽量避免选择同一组的品种相互杂交, 同时对于与其他来源地品种群体遗传距离较远的墨西哥品种群体在亲本选配时应给予更多关注。

Sugarcane varieties are important in providing parental resources in sugarcane breeding programs. In order to improve efficiency in selecting sugarcane varieties as parents, to provide guidance in choosing the best parent combinations for crossing, and to assist in choosing core collections of parents, the genetic variation, genetic structure and relationships of a range of traits were studied in a set of sugarcane varieties from different origins. Seventeen qualitative traits and five quantitative traits were studied, and a range of statistics were determined including genetic variance for each quantitative trait, genetic distance, coefficient of genetic divergence, and genotypic relationships based on cluster analysis. The coefficient of variance (CV) of all quantitative traits differed among different groups of varieties based on their origins, that population from Hainan Province exhibited the largest genetic diversity. The analysis of genetic diversity of qualitative traits revealed that variety populations from the USA, Taiwan of China, and Australia had high levels of genetic diversity in trait values, suggesting there had been greater use of diverse parental materials in programs in those places. Results from analysis of coefficients of genetic divergence (Gst = 0.0479 for quantitative traits, Gst = 0.0950 for qualitative traits) indicated that a high proportion of total genetic variation was retained within the groups of origin, with a high level of overlap in variation between most groups, and the high gene flow (Nm = 4.7632 for qualitative traits, Nm = 9.9493 for quantitative traits) showed the active genetic ex-change was found between different origins. Based on analysis of genetic distance, relationship between va-riety populations from different origins was examined. The genetic distance between origins ranged from 0.0261 to 0.2945, with the greatest similarity being evident in variety populations between Fujian and Guangdong provinces (China), Cuba and USA, Guangxi and Yunnan provinces (China), Australia and Phil-ippines, Jiangxi and Sichuan provinces (China), Brazil and France. This may reflect similarities in ancestors of parents used in these pairs of programs, in particular for the variety populations from Fujian and Guang-dong which were the most similar. It is suggested these sugarcane variety populations which possess rich phenotypic diversity should be utilized and those crosses between sugarcane variety populations within the same groups from cluster analysis should be avoided. Sugarcane variety populations from Mexico, which are quite dissimilar to most other groups, should be given a higher priority in breeding programs.

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