生物多样性 ›› 2001, Vol. 09 ›› Issue (3): 241-246.doi: 10.17520/biods.2001037

• 论文 • 上一篇    下一篇

中国对虾(Penaeus chinensis)4个种群的同工酶遗传变异(英文)

王伟继, 孔杰, 包振民, 邓景耀, 庄志猛   

  1. 1. 中国水产科学研究院黄海水产研究所!青岛266071
    2. 青岛海洋大学海洋生命学院!青岛266003
  • 出版日期:2001-08-20
  • 通讯作者: 王伟继

Isozyme variation in four populations of Penaeus chinensis shrimp

WANG Wei-Ji1 , KONG Jie1 , BAO Zhen-Min2 , DENG Jing-Yao1 , ZHUANG Zhi-Meng1   

  1. 1 Yellow Sea Fisheries Research Institute , Qingdao 266071
    2 Marine Life College , Ocean University of Qingdao , Qingdao 266003
  • Online:2001-08-20
  • Contact: WANG Wei-Ji

采用水平淀粉凝胶电泳技术分析了中国对虾 (Penaeuschinensis)黄渤海沿岸种群 (YP)、朝鲜半岛西海岸种群(KP)和 2个养殖种群 (CP1和CP2 )的同工酶遗传变异水平。每个种群随机选取 50尾中国对虾进行同工酶检测。在所分析的 12种同工酶编码的 2 0个基因位点中 ,有 4个是多态位点。 4个种群的多态位点比例 (P0 .99)分别为15 %、2 0 %、10 %和 2 0 %。种群平均杂合度 (观测值 ) (Ho)分别为 0 .0 14± 0 .0 0 7、0 .0 2 0± 0 .0 10、0 .0 10± 0 .0 0 7和0 .0 33± 0 .0 17。 4个种群的位点有效等位基因数 (Ne)分别为 1.0 15± 0 .0 0 8、1.0 2 3± 0 .0 11、1.011±0.007he 1.042±0.022。杂合子平衡偏离指数(D)分别为+0.037、-0.030、-0.098和-0.030。2个地理种群(YP和KP)的遗传形似性系数(I)和遗传距离(Dnei)分别为0.99998和0.00002

关键词: 白鱀豚, 漩涡模型, 种群生存能力分析, 野生动物管理

Horizontal starch gel elect rophoresis was used to investigate the isozyme genetic variation in Penaeus chinensis shrimp. Each 50 individuals from two wild geographic populations dist ributed in the China Coast of the Yellow Sea ( YP) and the western coast of Korean Peninsula ( KP) , and from two cultivated populations (CP1 and CP2) were surveyed. Of 20 loci encoding 12 enzymes , four are polymorphic. The mean proportions of polymorphic loci ( P0. 99) of YP , KP , CP1 and CP2 are 15 % , 20 % , 10 % and 20 % with the average heterozygosities ( Ho) of 0. 014 ±0. 007 , 0. 020 ±0. 010 , 0. 010 ±0. 007 and 0. 033 ±0. 017 , respectively. The mean effective allele number ( Ne) of these four populations are 1. 015 ±0. 008 , 1. 023 ±0. 011 , 1. 011 ±0. 007 and 1. 042 ±0. 022 while the heterozygous divergent indexes ( D) of them value + 0. 037 , - 0. 030 , - 0. 098 and - 0. 030 , respectively. The genetic similarity index ( I) and genetic distance ( Dnei) between two wild geographic populations are 0. 99998 and 0. 00002 , respectively.

Key words: Chinese River Dolphin, VORTEX model, population viability analysis, wildlife management

[1] 李欣海, 李典谟, 路宝忠, 翟天庆. (1996) 朱鹮(Nipponia nippon)种群生存力分析. 生物多样性, 04(2): 69-77.
[2] 张先锋, 王丁, 王克雄. (1994) 漩涡模型及其在白鱀豚种群管理中的应用. 生物多样性, 02(3): 133-139.
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