生物多样性 ›› 1998, Vol. 06 ›› Issue (4): 282-286.DOI: 10.17520/biods.1998042

• 论文 • 上一篇    下一篇

板栗在6个同工酶位点上的遗传变异

张辉,柳鎏,Villani F   

  1. (江苏省·中国科学院植物研究所,  南京 210014)
    ( Institute of Agriforestry , CNR 0510 Porano ( TR) , Italy)
  • 收稿日期:1997-05-13 修回日期:1998-12-11 出版日期:1998-11-20 发布日期:1998-11-20

A research on genetic variation of Castanea mollissima at six isozyme loci

Zhang Hui1), Liu Liu1), Villani F2)   

  1. 1) Jiangsu Institute of Botany , Chinese Academy of Sciences , Nanjing 210014
    2) Institute of Agriforestry , CNR 0510 Porano ( TR) , Italy
  • Received:1997-05-13 Revised:1998-12-11 Online:1998-11-20 Published:1998-11-20

摘要:

利用水平板淀粉凝胶电泳技术检验了板栗8个居群在6个同工酶位点(Per-2、Got-2、Est-2、Gpi-2、Idh-1、Idh-2 )上的遗传变异(多态位点百分率为97.9%,平均等位基因数为2.54,平均预期杂合值为0.452 ) 。在同一水平上,其遗传多样性水平显著高于欧洲栗。总的基因多样性中,89.2%发生在居群内,10.8%发生于居群间,各居群之间的遗传距离为0.036~0.394,有些居群分化较大,尤其在个别位点上更为明显。初步研究表明,其丰富的遗传变异及其高水平的分化度可能与其生境的多样性、风媒异交等因素造成的长距离的基因漂变有关。

关键词: 秦岭, 种子植物, 数据库

Abstract:

Allozyme genetic variation at six loci (Per-2, Got-2, Est-2, Gpi-2, Idh-1, Idh-2 ) in eight populations of C. mollissima was invested using starch gel electrophoresis. The result showed very high genetic variation in the populations with the proportion of polymorphic loci of 97.9% in the average expected heterozygosity of 0.452. In general, about 89.2% of the genetic variation was attributed within populations, while the remaining 10.8% among populations. The genetic distance ranged from 0.036~0.394. There existed obvious difference, especially in a few locus .The primary result indicted the high level of genetic variation and difference could be closely related to the long gene migration flow which attributed to the diversity of its habitat, the outcrossing-winded character and so on.

Key words: Qinling Mountain, seed plant, database