Abstract:

The genetic diversity of Paeonia delavayi, a species endemic to Southwest China, was analyzed using inter-simple sequence repeat markers (ISSR). A total of 116 bands were amplified from 16 natural populations and one ex situ conserved population by 10 informative and reliable primers of 100 pre-screened primers. Of them, 92 ISSR loci were polymorphic. A relatively high level of intraspecific genetic diversity was revealed: PPB = 44.61%,H = 0.1657 and I = 0.2448 at population level; PPB = 79.31%, H = 0.2947 and I = 0.4355 at species level. The G_ST = 0.4349 indicated a high degree of genetic differentiation occurred among P. delavayi populations. The results of genetic diversity study, combined with field survey and previous investigations, suggest that P. delavayi is not endangered.