生物多样性 ›› 2000, Vol. 08 ›› Issue (3): 337-342.  DOI: 10.17520/biods.2000048

• 论文 • 上一篇    下一篇

分子生物学方法在微生物多样性研究中的应用

杨永华,姚健   

  1. (南京大学生物科学与技术系,  南京 210093)
  • 收稿日期:1999-08-17 修回日期:2000-05-16 出版日期:2000-08-20 发布日期:2000-08-20
  • 通讯作者: 杨永华

Molecular techniques and their application to the study of microbial diversity

YANG Yong-Hua, YAO Jian   

  1. Department of Biological Sciences and Technology , Nanjing University , Nanjing  210093
  • Received:1999-08-17 Revised:2000-05-16 Online:2000-08-20 Published:2000-08-20
  • Contact: YANG Yong-Hua

摘要:

微生物多样性是生物多样性的重要组成部分。由于微生物和大生物(动、植物)相比,存在着多种显著差异,因此其多样性研究、保护及利用也有所不同,尤其是研究方法亟待完善、提高。近年来,分子生物学方法广泛用于微生物多样性的研究并取得了一系列研究成果。本文从四个方面加以介绍:1)微生物总DNA制备及其遗传多样性检测方法;2)16S rRNA基因序列研究;3)核酸杂交分析技术;4)DNA动力学的研究。今后的发展趋势是加强这些方法间及其与传统方法的有机结合,并发展新的方法,促进微生物多样性研究的深入开展。

AbstractMicrobial diversity is an important part of biodiversity. Microbial diversity research and its conservation and utilization are quite different from those of macroorganisms including animals and plants. In particular , it is necessary to develop new techniques suitable for microbial diversity research. Recently , as the development of modern molecular biology , several molecular techniques have been used to study microbial diversity , including 1) total DNA extraction of microbe and its genetic diversity assay , 2) 16S rRNA gene sequence analysis , 3) nucleic acid hybridization , and 4) DNA kinetics. In order to promote extensive and intensive research on microbial diversity , it’s necessary to integrate these methods to enhance a combination of traditional and modern techniques , and to develop novel method.