偏花报春和海仙报春3个同域居群的不对称杂交

doi:10.17520/biods.2017001

生物多样性 ›› 2017, Vol. 25 ›› Issue (6): 647-653. DOI: 10.17520/biods.2017001 cstr: 32101.14.biods.2017001

偏花报春和海仙报春3个同域居群的不对称杂交

谢艳萍¹, 赵建立², 朱兴福¹, 李莉¹, 李庆军^1,^2,^*()

1 中国科学院西双版纳热带植物园热带森林生态学重点实验室, 云南勐腊 666303
2云南大学生态学与进化生物学实验室, 云南省生物资源保护与利用国家重点实验室, 昆明 650091

收稿日期:2017-01-01 接受日期:2017-06-17 出版日期:2017-06-20 发布日期:2017-07-10
通讯作者: 李庆军
基金资助:
国家自然科学基金(31500194)和云南省联合基金(U1202261)

Asymmetric hybridization of Primula secundiflora and P. poissonii in three sympatric populations

Yanping Xie¹, Jianli Zhao², Xingfu Zhu¹, Li Li¹, Qingjun Li^1,^2,^*()

1 Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan 666303
2 Laboratory of Ecology and Evolutionary Biology, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091

Received:2017-01-01 Accepted:2017-06-17 Online:2017-06-20 Published:2017-07-10
Contact: Li Qingjun

摘要/Abstract

摘要：

自然杂交是进化生物学研究中的热点, 而杂交方向则是理解自然杂交和生殖隔离机制的一个关键环节。偏花报春(Primula secundifora)和海仙报春(P. poissonii)在横断山区重叠分布且存在自然杂交现象, 研究仅证实了种间杂交的发生, 但对不同地域的这2个物种间的杂交方向并未开展深入研究。本研究选取偏花报春和海仙报春3个同域分布点的居群, 利用双亲遗传的核转录间隔区ITS和母系遗传的叶绿体片段trnH-psbA构建系统树, 检测了3个居群中的自然杂交以及杂交方向; 通过人工授粉实验检测种内种间杂交以及不同物种作为母本时对结籽数的影响水平。DNA分子标记结果显示每个居群中的杂交方向都是不对称的, 海仙报春更容易在杂交中作为母本。人工授粉的结果表明种内种间交配以及哪个种作为母本对结籽数有非常显著的影响。海仙报春作为母本时能够显著提高结籽数。这2个物种之间不同的生殖隔离强度可能是造成不对称杂交的原因。3个居群中都是以海仙报春更易作母本, 可能是因为这3个地点的种间生殖隔离具有相似的机制。

关键词: 报春花属, 同域分布, 杂交方向, 不对称

Abstract

Natural hybridization is one of the research hotspots in evolutionary biology. The direction of hybridization is a critical aspect to understanding natural hybridization and interspecific reproductive isolation. Although natural hybridization between Primula secundiflora and P. poissonii has been confirmed in overlapping areas in the Hengduan Mountains, the interspecific hybridization directions among different populations remain unclear. We chose three sympatric populations to test interspecific hybridization directions. Biparentally inherited nuclear internal transcribed spacer (ITS) and maternally inherited chloroplast sequence trnH-psbA techniques were used to confirm hybridization and to test directions. Moreover, hand pollination of intraspecies and interspecies populations were performed to detect which factors influence seed numbers and hybridization patterns. Results of DNA marker analysis suggested that the direction of hybridization was asymmetric in each population, and P. poissonii was found to be the most maternal parent. Intraspecific crossing produced significantly more seeds than interspecific crossing. Primula poissonii produced significantly higher seed numbers when it was treated as the maternal parent. The asymmetric direction may be the result of the asymmetric strength of reproductive barriers between P. secundiflora and P. poissonii. Similar mechanisms of reproductive isolation may contribute to the asymmetric hybridization among the three sympatric sites.

Key words: Primula, sympatric distribution, hybridization direction, asymmetric

谢艳萍, 赵建立, 朱兴福, 李莉, 李庆军 (2017) 偏花报春和海仙报春3个同域居群的不对称杂交. 生物多样性, 25, 647-653. DOI: 10.17520/biods.2017001.

Yanping Xie, Jianli Zhao, Xingfu Zhu, Li Li, Qingjun Li (2017) Asymmetric hybridization of Primula secundiflora and P. poissonii in three sympatric populations. Biodiversity Science, 25, 647-653. DOI: 10.17520/biods.2017001.

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链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2017001

https://www.biodiversity-science.net/CN/Y2017/V25/I6/647

图/表 5

图1 香格里拉格咱乡(GZ)、普达措国家公园(PTS)和香格里拉高山植物园(SABG) 3个海仙报春和偏花报春居群的地理位置

Fig. 1 Locations of the three populations from Geza Village (GZ), Potatso National Park (PTS) and Shangri-La Alpine Botanical Garden (SABG) in Shangri-La

图2 海仙报春(A)、杂交个体(B)和偏花报春(C)核转录间隔区ITS序列中的双峰结构

Fig. 2 Double waves on part of internal transcribed spacer (ITS) sequences in Primula poissonii (A), Hybrids (B) and P. secundiflora (C)

表1 叶绿体片段trnH-psbA中杂交个体和亲本存在变异的位点及位置

Table 1 Positions at which hybrids and parents differ in the trnH-psbA sequence

居群 Population	物种 Species	个体数 No. of individuals	trnH-psbA变异位点及位置 Sequence region and position (bp) of trnH-psbA
居群 Population	物种 Species	个体数 No. of individuals	36 bp	63 bp	103 bp	112-386 bp
	海仙报春 Primula poissonii	7	G	C	A	1*
	偏花报春 P. secundiflora	7	A	A	T	2*
格咱 Geza Village (GZ)	杂交个体类型1 Hybrid type-1	6	G	C	A	1*
格咱 Geza Village (GZ)	杂交个体类型2 Hybrid type-2	4	A	A	T	2*
香格里拉高山植物园 Shangri-La Alpine Botanical Garden (SABG)	杂交个体类型1 Hybrid type-1	2	G	C	A	1*
普达措国家公园 Potatso National Park (PTS)	杂交个体类型1 Hybrid type-1	15	G	C	A	1*
普达措国家公园 Potatso National Park (PTS)	杂交个体类型2 Hybrid type-2	5	A	A	T	2*

图3 基于Bayes法构建的核转录间隔区ITS和叶绿体基因trnH-psbA系统树。GZ、PTS和SABG分别代表格咱乡、普达措国家公园和香格里拉高山植物园居群。

Fig. 3 The nuclear ITS and chloroplast trnH-psbA phylogeny tree based on Bayes method. GZ, PTS and SABG are the abbreviations of populations from Geza Village, Potatso National Park and Shangri-La Alpine Botanical Garden.

表2 种内种间交配对结籽数的影响

Table 2 Effects of cross-pollination treatments (within or between species and mother species) on seed production

来源 Source	Wald 卡方值	自由度	P值
	Wald Chi-Square	df	P-value
种内 vs. 种间 Within vs. between species (WBS)	61.110	1	< 0.001
海仙报春 vs. 偏花报春作母本 Primula poissonii vs. P. secundiflora as mother (PSM)	29.078	1	< 0.001
交互作用 Interaction (WBS × PSM)	22.074	1	< 0.001

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偏花报春和海仙报春3个同域居群的不对称杂交

Asymmetric hybridization of Primula secundiflora and P. poissonii in three sympatric populations

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