生物多样性 ›› 2025, Vol. 33 ›› Issue (4): 24584.  DOI: 10.17520/biods.2024584

• 生物编目 • 上一篇    下一篇

广东翼手目物种名录更新及分子条形码数据库构建

廖雅晴1,黄泽锋1,王晓云1,张礼标2,吴毅1*,余文华1*   

  1. 1.广州大学生命科学学院, 华南生物多样性保护与利用重点实验室, 广州 510006 2.广东省科学院动物研究所, 广东省动物保护与资源利用重点实验室, 广州 510260
  • 收稿日期:2024-12-28 修回日期:2025-02-11 出版日期:2025-04-20 发布日期:2025-03-27
  • 通讯作者: 余文华

An updated checklist of Chiroptera in Guangdong and the construction of a molecular barcode database

Yaqing Liao1,Zefeng Huang1,Xiaoyun Wang1,Libiao Zhang2,Yi Wu1*,Wenhua Yu1*   

  1. 1 School of Life Sciences of Guangzhou University, Key Laboratory of Conservation and Application in Biodiversity of South China, Guangzhou 510006, China 

    2 Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China

  • Received:2024-12-28 Revised:2025-02-11 Online:2025-04-20 Published:2025-03-27
  • Contact: Wenhua Yu

摘要: 广东是翼手目分布的热点区域,也是中国翼手目物种多样性最丰富的省份之一。为全面了解广东省翼手目多样性资源现状,给日后的生物多样性研究和保护提供基础资料,本研究以课题组长期本底调查与物种厘定结果为依据,结合广东历史记录文献,对本省翼手目名录进行梳理、订正和更新,针对性地基于本地标本序列构建广东省翼手目物种分子条形码数据库。结果显示,截至2024年7月,广东省记录翼手目8科24属61种,物种多样性仅次于云南(8科31属82种),占我国蝙蝠的43.2%。其中,蝙蝠科Vespertilionidae物种37种,为物种多样性最丰富的科;鼠耳蝠属Myotis物种11种,为物种多样性最丰富的属。本研究还运用DNA条形码技术,主要基于本省组织样品扩增得到的COICytb分子序列数据,建立了本省涵盖61种翼手类的分子条形码本底参考数据库。这不仅更新修订了广东省翼手目物种名录与分布现状,且所建立的分子数据库能为本省及周边省区的翼手目物种分子鉴定提供准确、便捷的基础数据。

关键词: 广东省, 翼手目(蝙蝠), 更新名录, 分子条形码, 分子数据库

Abstract

Aims: Guangdong is one of the provinces with the high chiropteran diversity in China and often serve as one of hot spots in Chiroptera researches. To comprehensively understand the current diversity resources of Chiroptera in Guangdong Province, and to provide basic data for future biodiversity research and conservation practices, the timely updating of species checklists and the availability and accuracy of molecular barcode databases, even those based on local species specimens, are necessary. 

Methods: This study is based on our long-term survey results over 30-years effort, combined with a summary of historical records and literature on Guangdong, we aim to update the chiropteran species checklist for the province. Additionally, we will selectively utilize our specimen resources that collected by our research team over 30-years to specifically construct their molecular barcode database via molecular barcoding technology. We provide primers and PCR systems for molecular barcoding technology that cover the known species of Chiroptera, facilitating future use by researchers. 

Results: As of July 2024, Guangdong Province has recorded 61 species of Chiroptera across 8 families and 24 genera, making its species diversity second only to Yunnan Province, which has 82 species across 8 families and 31 genera. Its diversity accounts for 43.2% of the bats in China. Among these, the family Vespertilionidae has the highest diversity with 37 species, while the genus Myotis is the most diverse genus with 11 species. We compiled 11 pairs of universal primers for the Cytb gene and 9 pairs for the COI gene. Based on molecular sequence data primarily obtained from samples that historically collected in this province, we have established a reference barbcoding database for the 61 Chiroptera species in Guangdong Province. 

Conclusions: This study not only revises and updates the chiropteran species checklist in Guangdong Province, but also establishes the molecular barcoding database. It could provides accurate and convenient basic data for the molecular identification of Chiroptera species in the province and the Guangdong-Hong Kong-Macao Greater Bay Area. We hope this will benefit future biodiversity research and conservation practice.

Key words: Guangdong Province, Chiroptera (bat), Updated Checklist, Molecular Barcode, Molecular Database