滇牡丹遗传多样性的ISSR分析

doi:10.1360/biodiv.040179

生物多样性 ›› 2005, Vol. 13 ›› Issue (2): 105-111. DOI: 10.1360/biodiv.040179

滇牡丹遗传多样性的ISSR分析

杨淑达^1,2, 施苏华³, 龚洵^1,3*, 周仁超³

1 （中国科学院昆明植物研究所，昆明650204）
2 （中国科学院研究生院，北京100039）
3 （中山大学生命科学学院，广州510275）

收稿日期:2004-11-22 修回日期:2004-12-17 出版日期:2005-03-20 发布日期:2005-03-20
通讯作者: 龚洵

Genetic diversity of Paeonia delavayi as revealed by ISSRs

Shuda Yang^1,2, Suhua Shi^３, Xun Gong^1,3*, Renchao Zhou³

1 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204
2 Graduate School of the Chinese Academy of Sciences, Beijing 100039
3 School of Life Sciences, Sun Yat-sen University, Guangzhou 510275

Received:2004-11-22 Revised:2004-12-17 Online:2005-03-20 Published:2005-03-20
Contact: Xun Gong

摘要/Abstract

摘要：

应用ISSR标记对中国西南地区特有植物滇牡丹(Paeonia delavayi)的遗传多样性进行了研究。从100个引物中筛选出10个用于正式扩增，在取自16个自然居群和1个迁地保护居群的511个个体中，检测到92个多态位点。在居群水平上，多态位点百分率（PPB）为44.61%，Nei′s基因多样性指数（H）和Shannon信息指数（I）分别为0.1657和0.2448。在物种水平上，多态位点百分率（PPB）为79.31%，Nei′s基因多样性指数（H）和Shannon信息指数（I）分别为0.2947和0.4355。居群间的遗传分化系数(G_ST)达0.4349。结果表明：滇牡丹遗传多样性水平较高，居群间遗传分化较大。结合以前的研究结果，对滇牡丹的现状进行评估的结果显示，滇牡丹并不濒危。

Abstract

The genetic diversity of Paeonia delavayi, a species endemic to Southwest China, was analyzed using inter-simple sequence repeat markers (ISSR). A total of 116 bands were amplified from 16 natural populations and one ex situ conserved population by 10 informative and reliable primers of 100 pre-screened primers. Of them, 92 ISSR loci were polymorphic. A relatively high level of intraspecific genetic diversity was revealed: PPB = 44.61%,H = 0.1657 and I = 0.2448 at population level; PPB = 79.31%, H = 0.2947 and I = 0.4355 at species level. The G_ST = 0.4349 indicated a high degree of genetic differentiation occurred among P. delavayi populations. The results of genetic diversity study, combined with field survey and previous investigations, suggest that P. delavayi is not endangered.

杨淑达, 施苏华, 龚洵, 周仁超 (2005) 滇牡丹遗传多样性的ISSR分析. 生物多样性, 13, 105-111. DOI: 10.1360/biodiv.040179.

Shuda Yang, Suhua Shi, Xun Gong, Renchao Zhou (2005) Genetic diversity of Paeonia delavayi as revealed by ISSRs. Biodiversity Science, 13, 105-111. DOI: 10.1360/biodiv.040179.

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滇牡丹遗传多样性的ISSR分析

Genetic diversity of Paeonia delavayi as revealed by ISSRs

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