生物多样性 ›› 2012, Vol. 20 ›› Issue (3): 308-316.DOI: 10.3724/SP.J.1003.2012.09025

所属专题: 传粉生物学:理论探讨与初步实践

• 方法 • 上一篇    下一篇

花颜色和花气味的量化研究方法

李庆良1, 马晓开2,3, 程瑾1*, 罗毅波2   

  1. 1北京林业大学生物科学与技术学院, 北京 100083
    2中国科学院植物研究所系统与进化植物学国家重点实验室, 北京 100093
    3中国科学院研究生院, 北京 100049
  • 收稿日期:2012-01-17 修回日期:2012-04-10 出版日期:2012-05-20 发布日期:2012-05-09
  • 通讯作者: 程瑾
  • 基金资助:

    北京林业大学青年科技启动基金;中央高校基本科研业务费专项资金;国家自然科学基金资助项目兰属建兰亚属植物中传粉综合征有效性检测及其花部构成吸引作用的量化研究

Quantitative studies of floral color and floral scent

Qingliang Li1, Xiaokai Ma2,3, Jin Cheng1*, Yibo Luo2   

  1. 1College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083

    2State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093

    3Graduate University of the Chinese Academy of Sciences, Beijing 100049
  • Received:2012-01-17 Revised:2012-04-10 Online:2012-05-20 Published:2012-05-09
  • Contact: Jin Cheng
  • Supported by:

    ;the Fundamental Research Funds for the Central Universities

摘要: 花颜色和花气味是花部构成的重要内容。在已开展的传粉生态学研究中对二者的报道主要是描述性的, 而其量化研究可以为揭示传粉机制提供有力的实验证据。本文主要介绍了花颜色的测量和标定方法, 包括比色卡、分光色差仪和便携式光谱仪等; 花气味的采集方法, 包括动态顶空套袋–吸附采集法、吸附–溶剂洗脱法和固相微萃取法等; 花气味的检测和分析方法, 包括气相色谱–质谱联用仪分析和电子鼻型超速气相色谱仪分析等; 以及昆虫行为学实验方法, 包括气相色谱–昆虫触角电位联用技术、Y型嗅觉仪和飞行箱实验等。科研人员可以根据实验材料的特点和实验目的选择适合的量化研究方法。

Abstract: Floral color and scent are two important components of floral design. Although previous studies on floral design have been mostly descriptive. The exploration on their quantitative studies can provide key evidence to learn pollination mechanisms. Our study focuses on recently developed methods for the quantitative measurement of floral color and floral scent. We introduce three floral color measurement and plotting methods, including color chart, colorimeter and spectrometer. Dynamic headspace collection-adsorption, adsorption-solvent desorption and solid phase micro-extraction are the three frequently-used floral scent collection methods and gas chromatography-mass spectrometry and zNose are usually used in floral scent analysis. Lastly, gas chromatography with electroantennographic detector, Y-tube olfactometer and flight cage experiment can help us to evaluate insect behavior.