生物多样性 ›› 2000, Vol. 08 ›› Issue (3): 257-261.  DOI: 10.17520/biods.2000036

• 论文 • 上一篇    下一篇

螅状独缩虫(Cachesium polypinum)遗传多样性的 RAPD 分析

张锡元,孙翀,晏婷婷,吴海,郑翌,李晓迎,缪炜,余育和,沈韫芬   

  1. (武汉大学生命科学学院, 　武汉　430072)
    (中国科学院水生生物研究所, 　武汉　430072)
  • 收稿日期:2000-03-01 修回日期:2000-06-19 出版日期:2000-08-20 发布日期:2000-08-20
  • 通讯作者: 张锡元

RAPD analysis of genetic diversity of Cachesium polypinum

ZHANG Xi-Yuan1), SUN Chong1), YAN Ting-Ting1), WU Hai1), ZHENG Yi1), LI Xiao-Ying1), MIAO Wei2), YU Yu-He2), SHEN Yun-Fen2)   

  1. 1) College of Life Sciences ,Wuhan University , Wuhan 　430072
    2) Institute of Hydrobiology , Chinese Academy of Sciences , Wuhan 　430072
  • Received:2000-03-01 Revised:2000-06-19 Online:2000-08-20 Published:2000-08-20
  • Contact: ZHANG Xi-Yuan

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2493

被引次数

3

摘要：

用随机扩增多态DNA（RAPD）技术对武汉的汉口（CP－1）、汉阳（CP－2）和武昌（CP－3）三个地区缘毛类的螅状独缩虫（Cachesium polypinum）进行了遗传多样性分析。结果表明：这种动物存在广泛的变异，三个地域群体之间的遗传相似度为 0.6522～0.7385（平均值为 0．6921），各群体之间遗传相似度大小依次为：CP- 2—CP－3＞CP－1—CP－2＞CP－1—CP－3。本文对这些变异与环境的关系和以RAPD技术研究缘毛类等动物遗传多样性的意义作了分析与讨论。

AbstractThree regional populations (Hankou :CP-1 ,Hanyang :CP-2 ,Wuchang :CP-3) of Cachesium polypinum in Wuhan were analysed using randomly amplified polymorphic DNA ( RAPD) technique. Based on RAPD data , the pairwise genetic similarity of the three populations were 0.6921 on average , ranging from 0.6522 to 0.7385. The genetic similarity (S) between CP-2 and CP-3 was the greatest ,and S between CP-1 and CP-3 was the least . The relationship between variations and environment , and the significance of RAPD analysis of genetic diversity of Cachesium polypinum were discussed.

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