生物多样性 ›› 1999, Vol. 07 ›› Issue (3): 197-201.  DOI: 10.17520/biods.1999030

• 论文 • 上一篇    下一篇

海带(Laminaria japonica)丝状体遗传多样性的比较研究

王莹,戴继勋   

  1. (河南职业技术师范学院, 新乡 453003)
     (青岛海洋大学, 青岛 266003)
  • 收稿日期:1998-04-08 修回日期:1999-03-29 出版日期:1999-08-20 发布日期:1999-08-20

Comparison of genetic diversity in Laminaria japonica gametophytes

WANG Ying, DAI Ji-Xun   

  1. 1) Henan Vocation2Technical Normal College , Xinxiang  453003
    2) Qingdao Oceanography University , Qingdao  266003
  • Received:1998-04-08 Revised:1999-03-29 Online:1999-08-20 Published:1999-08-20

摘要:

采用垂直板式聚丙烯酰胺凝胶电泳技术对分别来自中国青岛与日本北海道海带(Laminaria japonica)丝状体的6种酶系统(乙醇脱氢酶、谷氨酸脱氢酶、异柠檬酸脱氢酶、乳酸脱氢酶、山梨醇脱氢酶和超氧化物歧化酶)进行了检测,发现存在22个基因位点,分析了不同来源海带丝状体的遗传多样性与遗传分化程度。结果表明:日本北海道海带丝状体的预期杂合度、每个位点有效等位基因数等遗传参数均大于中国青岛海带丝状体。对日本北海道海带丝状体与中国青岛海带丝状体的遗传相似系数和遗传距离进行了计算分析,显示两者发生了遗传分化。日本北海道海带丝状体的遗传多样性高于中国青岛海带丝状体。研究结果还发现在中国青岛海带丝状体的Idh、Ldh 和Sdh中,以及日本北海道海带丝状体的Adh、Gdh、Idh、Ldh、Sdh和Sod中存在着不同迁移率的不等距双带酶,可能由特殊基因控制,并集中在第3个基因位点上。

AbstractGenetic diversity was compared and analyzed between Laminaria japonica gametophytes from Qingdao of China and those from Hokkaido of Japan. Six enzyme systems ( Adh , Gdh , Idh , Lh , Sdh , and Sod) were examined by PAGE , and twenty two gene loci were found. The results showed that the genetic diversity in the gametophytes of Hokkaido was higher than that of Qingdao. There was genetic differentiation in gametophytes between Hokkaido and Qingdao. Double banding patterns of allozymes with different mobility were found for Qingdao gametophytes in Idh , Ldh , and Sdh enzymes as well as for Hokkaido gametophytes in all six enzymes. The bands may be controlled by special genes which mainly exist in the third gene loci.