生物多样性 ›› 1998, Vol. 06 ›› Issue (4): 278-281.DOI: 10.17520/biods.1998041

• 论文 • 上一篇    下一篇

猕猴桃属(Actinidia)植物的离体种质保存

陈维伦,郭东红,安和祥,朱至清   

  1. (中国科学院植物研究所,  北京 100093)
  • 收稿日期:1997-11-12 修回日期:1998-04-01 出版日期:1998-11-20 发布日期:1998-11-20

In vitro conservation of Actinidia

CHEN Wei-Lun, GUO Dong-Hong, AN He-Xiang, ZHU Zhi-Qing   

  1. Institute of Botany , Chinese Academy of Sciences , Beijing  100093
  • Received:1997-11-12 Revised:1998-04-01 Online:1998-11-20 Published:1998-11-20

摘要:

本文研究了猕猴桃属植物离体种质资源保存的方法。在一年中于盛夏时采取的植物材料在离体培养时能得到最好的效果;改进的剥离茎尖的方法使污染率大大降低,在MS附加BA 0.5,Z 0.1,GA 0.1~0.5 mg/L,蔗糖3%,琼脂0.55%的培养基中茎尖生长良好并且不产生不定芽。通过茎尖培养方法已保存了10多个种,40多个猕猴桃的离体种质。

Abstract

This paper deals with the methods for in vitro conservation of  Actinidia. Early summer was the best time to obtain the plant materials.The contamination rate was greatly decreased by using a improved technology to excise the shoot tips and the shoot tips grew well and had no adventitious shoot formation in MS medium supplemented with BA 0.5, Z 0.1, GA 0.1~0.5 mg/L.Up to date, about 12 species and more than 40 clones of Actinidia plants have been conserved in vitro.