生物多样性 ›› 1998, Vol. 06 ›› Issue (3): 186-190.DOI: 10.17520/biods.1998028

• 论文 • 上一篇    下一篇

中华绒螯蟹遗传变异的RAPD分析

高志千,周开亚   

  1. (南京师范大学生物多样性与分子进化研究室,  南京 210097)
  • 收稿日期:1997-09-09 修回日期:1998-02-04 出版日期:1998-08-20 发布日期:1998-08-20

Genetic variation of the Chinese mitten-handed crab (Eriocheir sinensis) populations detected by RAPD analysis

GAO Zhi-Qian, ZHOU Kai-Ya   

  1. Biodiversity and Molecular Evolution Laboratory , Nanjing Normal University , Nanjing 210097
  • Received:1997-09-09 Revised:1998-02-04 Online:1998-08-20 Published:1998-08-20

摘要:

用31个可重复性好的随机引物对中华绒螯蟹(Eriocheir sinensis)的辽河、瓯江和长江种群进行了RAPD分析。121个扩增片段中有27个多态片段。遗传距离指数(D)表明中华绒螯蟹种内遗传变异较低(D=0.004~0.063)。3个种群中,辽河种群(D=0.018)和瓯江种群(D=0.025)遗传变异较高,而长江种群遗传变异较低(D=0.013);辽河种群和瓯江种群间遗传距离(D=0.023)小于它们与长江种群间的遗传距离(D值分别为0.028和0.032)。提示人类经济活动可能已使这2个种群发生了基因交流。

AbstractRAPD was employed to assess the genetic variation among Eriochei r sinensis populations of the Yangtze , Liaohe and Oujiang river systems. 31 random reproducible primers revealed 121 amplified fragments in which 27 were polymorphic. Intraspecific genetic variation was low ( D = 01004~01063) . Intrapopulation genetic variation of Liaohe population and Oujiang population were higher than that of Yangtze population while the interpopulation variation between Liaohe and Oujiang populations was lower than that between either of them and Yangtze population. The results suggest that the human economic activities have probably caused gene exchanges between the two populations.